Apoptosis- and endoplasmic reticulum stress-related genes were regulated by estrogen and progesterone in the uteri of calbindin-D9k and -D28k knockout mice

2011 ◽  
Vol 113 (1) ◽  
pp. 194-203 ◽  
Author(s):  
Eui-Man Jung ◽  
Beum-Soo An ◽  
Kyung-Chul Choi ◽  
Eui-Bae Jeung
Keyword(s):  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Knockout Mice ◽  
Calbindin D9k ◽  
Stress Related Genes

114 ENDOPLASMIC RETICULUM (ER) STRESS IN HYPOXIA-INDUCED DIABETES MELLITUS MODEL

2016 ◽  
Vol 28 (2) ◽  
pp. 187
Author(s):  
C. Ahn ◽  
D. Lee ◽  
K. P. Kim ◽  
M. H. Lee ◽  
E.-B. Jeung
Keyword(s):  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Er Stress ◽  
Pancreatic Beta Cells ◽  
Hypoxic Condition ◽  
Protein Translation ◽  
Ion Concentration ◽  
Cellular Stress Response ◽  
Stress Markers ◽  
Calbindin D9k

Endoplasmic reticulum (ER) regulates calcium ion concentration as a reservoir in the cell. ER stress is a cellular stress response related to the endoplasmic reticulum. At the initial stage of ER stress, ER tries to restore normal function by halting protein translation, degrading misfolded proteins, and increasing production of chaperones involved in protein folding. If ER fails to restore ER stress, ER stress can lead cells to apoptosis. To study the signaling between ER stress and calcium channels under ER-stressed circumstances, we designed a hypoxia-induced diabetic model. Nine-week-old male mice were chosen, maintained under hypoxic condition under 10% O2, 5% CO2 for 10 days, and the expression of ER stress markers and calcium channel gene expression were examined by real-time PCR. By maintaining hypoxic condition, the mice showed high glucose levels. Under this diabetic condition, in pancreatic beta cells, ER stress markers were elevated. This tendency showed an increase in calbindin-D9k KO mice. Chaperones such as calreticulin and calnexin were decreased, but in calbindin-D9k KO mice chaperone calnexin was not decreased. Interestingly, the calbindin-D9k KO normoxia mice showed increased glucose level compared with wild-type normoxia mice. Also, calnexin expression of pancreas was decreased in calbindin-D9k KO normoxia mice. This result indicates that pancreas cells were under endoplasmic reticulum stress. Taken together, calbindin may play an important role in endoplasmic reticulum stress in pancreas. This work was supported by the National Research Foundation of Korea (NRF) grant of Korean government (MEST) (No. 2013-010514).

scholarly journals Mediterranean Diet Supplemented With Coenzyme Q10 Modifies the Expression of Proinflammatory and Endoplasmic Reticulum Stress–Related Genes in Elderly Men and Women

2011 ◽  
Vol 67A (1) ◽  
pp. 3-10 ◽  
Author(s):  
Elena M. Yubero-Serrano ◽  
Lorena Gonzalez-Guardia ◽  
Oriol Rangel-Zuñiga ◽  
Javier Delgado-Lista ◽  
Francisco M. Gutierrez-Mariscal ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Mediterranean Diet ◽  
Coenzyme Q10 ◽  
Elderly Men ◽  
Men And Women ◽  
Stress Related Genes
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