Towards in situ tissue repair: Human mesenchymal stem cells express chemokine receptors CXCR1, CXCR2 and CCR2, and migrate upon stimulation with CXCL8 but not CCL2

2007 ◽  
Vol 101 (1) ◽  
pp. 135-146 ◽  
Author(s):  
Jochen Ringe ◽  
Sandra Strassburg ◽  
Katja Neumann ◽  
Michaela Endres ◽  
Michael Notter ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Chemokine Receptors ◽  
Tissue Repair ◽  
Human Mesenchymal Stem Cells

Human Mesenchymal Stem Cells Constitutively Express Chemokines and Chemokine Receptors That Can Be Upregulated by Cytokines, IFN-β, and Copaxone

2007 ◽  
Vol 27 (1) ◽  
pp. 53-64 ◽  
Author(s):  
Juliana Croitoru-Lamoury ◽  
Francois M.J. Lamoury ◽  
John J. Zaunders ◽  
Laura A. Veas ◽  
Bruce J. Brew
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Chemokine Receptors ◽  
Human Mesenchymal Stem Cells

scholarly journals Mechanisms of Action of Human Mesenchymal Stem Cells in Tissue Repair Regeneration and their Implications

2017 ◽  
Vol 53 (02) ◽  
pp. 104-120 ◽  
Author(s):  
Manisha Singh ◽  
Suchi Gupta ◽  
Sonali Rawat ◽  
Swati Midha ◽  
Krishan Gopal Jain ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Tissue Repair ◽  
Molecular Mechanisms ◽  
Human Mesenchymal Stem Cells ◽  
Mechanisms Of Action ◽  
Maximum Output ◽  
Cell Replacement Therapy ◽  
Tissue Repair And Regeneration

ABSTRACTCell replacement therapy holds a promising future in the treatment of degenerative diseases related to neuronal, cardiac and bone tissues. In such kind of diseases, there is a progressive loss of specific types of cells. Currently the most upcoming and trusted cell candidate is Mesenchymal Stem Cells (MSCs) as these cells are easy to isolate from the tissue, easy to maintain and expand and no ethical concerns are linked. MSCs can be obtained from a number of sources like bone marrow, umbilical cord blood, umbilical cord, dental pulp, adipose tissues, etc. MSCs help in tissue repair and regeneration by various mechanisms of action like cell differentiation, immunomodulation, paracrine effect, etc. The future of regenerative medicine lies in tissue engineering and exploiting various properties to yield maximum output. In the current review article, we have targeted the repair and regeneration mechanisms of MSCs in neurodegenerative diseases, cardiac diseases and those related to bones. Yet there is a lot to understand, discover and then understand again about the molecular mechanisms of MSCs and then applying this knowledge in developing the therapy to get maximum repair and regeneration of concerned tissue and in turn the recovery of the patient.

scholarly journals Graphene Oxide Induced Osteogenesis Quantification by In-Situ 2D-Fluorescence Spectroscopy

2018 ◽  
Vol 19 (11) ◽  
pp. 3336 ◽  
Author(s):  
Valentina Palmieri ◽  
Marta Barba ◽  
Lorena Di Pietro ◽  
Claudio Conti ◽  
Marco De Spirito ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Graphene Oxide ◽  
Human Mesenchymal Stem Cells ◽  
Alizarin Red S ◽  
Alizarin Red ◽  
Tissue Sections ◽  
Osteogenic Induction ◽  
Calcium Deposits

Graphene and graphene oxide can promote the adhesion, growth and differentiation of mesenchymal stem cells. Further, graphene surface coatings accelerate the differentiation of human mesenchymal stem cells acting as osteogenic inducers. Quantification of the osteogenic induction is conventionally performed with Alizarin Red S (ARS), an anthraquinone derivative used to identify calcium deposits in tissue sections and cell cultures. The ARS staining is quite versatile because the dye forms an Alizarin Red S–calcium complex that can be extracted from the stained monolayer of cells and readily assayed by absorbance measurements. Direct visualization of stained deposits is also feasible; however, an in-situ visualization and quantification of deposits is possible only on transparent supports and not on thick opaque materials like ceramics and graphene composites that are well-known inducers of osteogenesis. In this manuscript, the shape of the 2D-fluorescence spectra of the ARS-calcium complex is used to develop a method to detect and monitor the in-situ differentiation process occurring during the osteogenic induction mediated by opaque graphene oxide surfaces.

Sign in / Sign up

Export Citation Format

Share Document