High resolution, high-throughput amide deuterium exchange-mass spectrometry (DXMS) determination of protein binding site structure and dynamics: Utility in pharmaceutical design

2001 ◽  
Vol 84 (S37) ◽  
pp. 89-98 ◽  
Author(s):  
Virgil L. Woods ◽  
Yoshitomo Hamuro
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Protein Binding ◽  
Binding Site ◽  
High Throughput ◽  
Site Structure ◽  
Exchange Mass Spectrometry ◽  
Structure And Dynamics ◽  
Deuterium Exchange Mass Spectrometry

scholarly journals Higher-order structural interrogation of antibodies using middle-down hydrogen/deuterium exchange mass spectrometry

2016 ◽  
Vol 7 (2) ◽  
pp. 1480-1486 ◽  
Author(s):  
Jingxi Pan ◽  
Suping Zhang ◽  
Albert Chou ◽  
Christoph H. Borchers
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Structural Characterization ◽  
Higher Order ◽  
Subzero Temperature ◽  
Hydrogen Deuterium Exchange ◽  
Exchange Mass Spectrometry ◽  
Hydrogen Deuterium ◽  
Deuterium Exchange Mass Spectrometry

Specific restricted proteolysis combined with subzero temperature HPLC and online ETD facilitates structural characterization of antibodies at high resolution.

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