Modeling of platelet concentrate yield in continuous-flow cell separation devices

1988 ◽  
Vol 4 (4) ◽  
pp. 188-193 ◽  
Author(s):  
Jeane P. Hester ◽  
Gerard J. Ventura
Keyword(s):  
Continuous Flow ◽  
Cell Separation ◽  
Flow Cell ◽  
Platelet Concentrate ◽  
Concentrate Yield ◽  
Separation Devices

scholarly journals Principles of blood separation and component extraction in a disposable continuous-flow single-stage channel

Blood ◽  
1979 ◽  
Vol 54 (1) ◽  
pp. 254-268 ◽  
Author(s):  
JP Hester ◽  
RM Kellogg ◽  
AP Mulzet ◽  
VR Kruger ◽  
KB McCredie ◽  
...  
Keyword(s):  
Whole Blood ◽  
Continuous Flow ◽  
Cell Separation ◽  
Flow Cell ◽  
Buffy Coat ◽  
Single Stage ◽  
Flow Rates ◽  
Blood Separation ◽  
Final Yield ◽  
Component Extraction

Abstract A single-stage disposable channel and seal that provides for leukocyte and granulocyte collection by continuous-flow cell separation (CFCS) has been designed by the IBM Corporation. This paper describes (1) the separation characteristics of whole blood as it responds to varying gravitational (G) forces and flow rates through the channel; (2) the mechanism by which the buffy coat accumulates and is extracted; (3) the efficiency of extraction; (4) those donor and procedural variables that contribute to the final yield; (5) posttransfusion increment response in patients; and (6) the functional integrity of the cells collected.

Continuous Flow Cell Separation From Human Peripheral Blood Using Dielectrophoresis and Numerical Analysis

2013 ◽  
Author(s):  
Sungho Kim ◽  
Youngdo Jung ◽  
Bruno Frazier ◽  
Don Giddens
Keyword(s):  
Continuous Flow ◽  
Cell Separation ◽  
Coupling Effect ◽  
Human Peripheral Blood ◽  
Unmet Need ◽  
Cell Manipulation ◽  
Electrode Design ◽  
Characteristic Features ◽  
Separation Devices ◽  
Discontinuous Mode

Cell manipulation using dielectrophoresis (DEP) has a great potential for biological applications in diagnostic devices because it has a capability to separate specific cells from mixed heterogeneous solutions, e.g., whole blood, selectively based on intrinsic dielectrophoretic characteristics. Thus, it does not require additional chemical tagging, which can lead to undesirable cell responses (1). Previous DEP cell separation has been run in a discontinuous mode, which may result in more complicated procedures, so that developing continuous flow DEP separation devices in practical sizes for larger throughput clinical applications is an unmet need. In this research, we model numerically a continuous flow dielectrophoresis (CF-DEP) device that was developed with novel angled-electrode design in a MEMs platform, and a pulsing mode was introduced between neighboring electrodes to obtain efficient cell separation. This allows examination of characteristic features of the electrode design and the coupling effect of dielectric and hydraulic forces to particle movement.

Human bone marrow processing using a new continuous-flow cell separation device

Transfusion ◽  
2015 ◽  
Vol 56 (4) ◽  
pp. 899-904 ◽  
Author(s):  
Martin G. Guttridge ◽  
Christopher Bailey ◽  
Christopher Sidders ◽  
Jennifer Nichols ◽  
Jane Bromham ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Continuous Flow ◽  
Cell Separation ◽  
Flow Cell ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Separation Device

Principles of Continuous Flow Cell Separation in a Circumferential Flow Disposable Channel

1980 ◽  
pp. 101-113 ◽  
Author(s):  
Jeane P. Hester ◽  
Robert M. Kellogg ◽  
Alfred Mulzet ◽  
Kenneth B. McCredie ◽  
Emil J. Freireich
Keyword(s):  
Continuous Flow ◽  
Cell Separation ◽  
Flow Cell

scholarly journals Principles of blood separation and component extraction in a disposable continuous-flow single-stage channel

Blood ◽  
1979 ◽  
Vol 54 (1) ◽  
pp. 254-268 ◽  
Author(s):  
JP Hester ◽  
RM Kellogg ◽  
AP Mulzet ◽  
VR Kruger ◽  
KB McCredie ◽  
...  
Keyword(s):  
Whole Blood ◽  
Continuous Flow ◽  
Cell Separation ◽  
Flow Cell ◽  
Buffy Coat ◽  
Single Stage ◽  
Flow Rates ◽  
Blood Separation ◽  
Final Yield ◽  
Component Extraction

A single-stage disposable channel and seal that provides for leukocyte and granulocyte collection by continuous-flow cell separation (CFCS) has been designed by the IBM Corporation. This paper describes (1) the separation characteristics of whole blood as it responds to varying gravitational (G) forces and flow rates through the channel; (2) the mechanism by which the buffy coat accumulates and is extracted; (3) the efficiency of extraction; (4) those donor and procedural variables that contribute to the final yield; (5) posttransfusion increment response in patients; and (6) the functional integrity of the cells collected.

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