A new continuous-flow cell separation method based on cell density: Principle, apparatus, and preliminary application to separation of human buffy coat

2001 ◽  
Vol 16 (4) ◽  
pp. 186-191 ◽  
Author(s):  
Yoichiro Ito ◽  
Kazufusa Shinomiya
Keyword(s):  
Cell Density ◽  
Continuous Flow ◽  
Cell Separation ◽  
Flow Cell ◽  
Buffy Coat ◽  
Separation Method ◽  
Density Principle ◽  
Preliminary Application

scholarly journals Principles of blood separation and component extraction in a disposable continuous-flow single-stage channel

Blood ◽  
1979 ◽  
Vol 54 (1) ◽  
pp. 254-268 ◽  
Author(s):  
JP Hester ◽  
RM Kellogg ◽  
AP Mulzet ◽  
VR Kruger ◽  
KB McCredie ◽  
...  
Keyword(s):  
Whole Blood ◽  
Continuous Flow ◽  
Cell Separation ◽  
Flow Cell ◽  
Buffy Coat ◽  
Single Stage ◽  
Flow Rates ◽  
Blood Separation ◽  
Final Yield ◽  
Component Extraction

Abstract A single-stage disposable channel and seal that provides for leukocyte and granulocyte collection by continuous-flow cell separation (CFCS) has been designed by the IBM Corporation. This paper describes (1) the separation characteristics of whole blood as it responds to varying gravitational (G) forces and flow rates through the channel; (2) the mechanism by which the buffy coat accumulates and is extracted; (3) the efficiency of extraction; (4) those donor and procedural variables that contribute to the final yield; (5) posttransfusion increment response in patients; and (6) the functional integrity of the cells collected.

scholarly journals Principles of blood separation and component extraction in a disposable continuous-flow single-stage channel

Blood ◽  
1979 ◽  
Vol 54 (1) ◽  
pp. 254-268 ◽  
Author(s):  
JP Hester ◽  
RM Kellogg ◽  
AP Mulzet ◽  
VR Kruger ◽  
KB McCredie ◽  
...  
Keyword(s):  
Whole Blood ◽  
Continuous Flow ◽  
Cell Separation ◽  
Flow Cell ◽  
Buffy Coat ◽  
Single Stage ◽  
Flow Rates ◽  
Blood Separation ◽  
Final Yield ◽  
Component Extraction

A single-stage disposable channel and seal that provides for leukocyte and granulocyte collection by continuous-flow cell separation (CFCS) has been designed by the IBM Corporation. This paper describes (1) the separation characteristics of whole blood as it responds to varying gravitational (G) forces and flow rates through the channel; (2) the mechanism by which the buffy coat accumulates and is extracted; (3) the efficiency of extraction; (4) those donor and procedural variables that contribute to the final yield; (5) posttransfusion increment response in patients; and (6) the functional integrity of the cells collected.

scholarly journals Blood Cell Separation in the Dog by Continuous Flow Centrifugation

Blood ◽  
1968 ◽  
Vol 31 (5) ◽  
pp. 653-672 ◽  
Author(s):  
DEAN BUCKNER ◽  
ROBERT EISEL ◽  
SEYMOUR PERRY
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Continuous Flow ◽  
Cell Separation ◽  
Mononuclear Cells ◽  
Buffy Coat ◽  
Donor Blood ◽  
The Mean ◽  
Blood Volumes

Abstract A closed continuous flow centrifuge was used to separate and collect large quantities of buffy coat cells from the dog. One hundred fifty-five separate centrifugations of 2-12 hours duration were performed. Up to 61.0 liters of blood, representing 2.2-52 donor blood volumes, were processed. Buffy coat cells with a preponderance of granulocytes or lymphocytes, were collected while plasma and red blood cells were returned to the donor without change in flow or gravitational field. The mean total number of leukocytes, granulocytes, mononuclear cells and platelets removed was 24.0, 17.0, 7.0 and 197.0 x 10,9 respectively, which was 34.0, 28.0, 66.0 and 36.0 percent, respectively, of each cell population entering the centrifuge. The effect of centrifugation on blood components was evaluated. Granulocytes from buffy coat collections exhibited normal phagocytic ability in vitro. The transfusion of large quantities of granulocytes (15.0 x 109) into leukopenic dogs produced an increment in peripheral granulocyte count (2.7 x 103 per mm.3). Granulocytes labeled with 3H-DFP were also infused with a recovery of 38.6 percent at one hour and a T-½ of 4-6 hours. Machine-separated lymphocytes responded normally to phytohemagglutinin. Prolonged passage of blood through the pumps, tubing and bowl without centrifugation resulted in a decrease in circulating platelet levels (21 percent). A greater decrease in platelets occurred with centrifugation at high g. forces (49 percent). Hemolysis of red blood cells was not a serious problem.

Sequential Field-Flow Cell Separation Method in a Dielectrophoretic Chip With 3-D Electrodes

2007 ◽  
Vol 16 (5) ◽  
pp. 1120-1129 ◽  
Author(s):  
Liming Yu ◽  
C. Iliescu ◽  
Guolin Xu ◽  
F.E.H. Tay
Keyword(s):  
Cell Separation ◽  
Flow Cell ◽  
Separation Method

Human bone marrow processing using a new continuous-flow cell separation device

Transfusion ◽  
2015 ◽  
Vol 56 (4) ◽  
pp. 899-904 ◽  
Author(s):  
Martin G. Guttridge ◽  
Christopher Bailey ◽  
Christopher Sidders ◽  
Jennifer Nichols ◽  
Jane Bromham ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Continuous Flow ◽  
Cell Separation ◽  
Flow Cell ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Separation Device

Principles of Continuous Flow Cell Separation in a Circumferential Flow Disposable Channel

1980 ◽  
pp. 101-113 ◽  
Author(s):  
Jeane P. Hester ◽  
Robert M. Kellogg ◽  
Alfred Mulzet ◽  
Kenneth B. McCredie ◽  
Emil J. Freireich
Keyword(s):  
Continuous Flow ◽  
Cell Separation ◽  
Flow Cell
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