Zinc oxide nanoparticles from Cyperus rotundus attenuates diabetic retinopathy by inhibiting NLRP3 inflammasome activation in STZ‐induced diabetic rats

2020 ◽  
Vol 34 (12) ◽  
Author(s):  
Liwei Zhang ◽  
Wen Chu ◽  
Lei Zheng ◽  
Juanjuan Li ◽  
Yuling Ren ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Diabetic Retinopathy ◽  
Nlrp3 Inflammasome ◽  
Zinc Oxide Nanoparticles ◽  
Diabetic Rats ◽  
Cyperus Rotundus ◽  
Oxide Nanoparticles ◽  
Inflammasome Activation ◽  
Nlrp3 Inflammasome Activation

scholarly journals Skin damage induced by zinc oxide nanoparticles combined with UVB is mediated by activating cell pyroptosis via the NLRP3 inflammasome–autophagy–exosomal pathway

2022 ◽  
Vol 19 (1) ◽  
Author(s):  
Yu-Ying Chen ◽  
Yu-Hsuan Lee ◽  
Bour-Jr Wang ◽  
Rong-Jane Chen ◽  
Ying-Jan Wang
Keyword(s):  
Zinc Oxide ◽  
Nlrp3 Inflammasome ◽  
Zinc Oxide Nanoparticles ◽  
Skin Toxicity ◽  
Oxide Nanoparticles ◽  
Autophagic Flux ◽  
Skin Thickness ◽  
Inflammasome Activation ◽  
Skin Damage ◽  
Nlrp3 Inflammasome Activation

Abstract Background Zinc oxide nanoparticles (ZnONPs) are widely used nanomaterial in personal cosmetics, such as skin creams and sunscreens, due to their whitening properties and strong UV light absorption. However, the safety issues and the hazards of ZnONPs, which can be taken up by the skin and cause skin toxicity, are still unclear. From a chemoprevention point of view, pterostilbene (PT) has been reported to prevent skin damage effectively by its anti-inflammatory and autophagy inducer effect. This study aims to determine the skin toxicity and the potential mechanisms of UVB and ZnONPs exposure and the preventive effect of PT. Results The co-exposure of UVB and ZnONPs elicit NLRP3 inflammasome activation and pyroptosis in keratinocytes. Furthermore, exposure to both UVB and ZnONPs also disrupts cellular autophagy, which increases cell exosome release. In vivo UVB and ZnONPs exposure triggers skin toxicity, as indicated by increased histological injury, skin thickness and transepidermal water loss. Notably, the NLRP3 inflammasome-mediated pyroptosis are also activated during exposure. Topical application of pterostilbene attenuates NLRP3 inflammasome activation and pyroptosis by decreasing ROS generation and mitochondrial ROS (mtROS) levels. In addition to its antioxidant effect, PT also reversed autophagy abnormalities by restoring normal autophagic flux and decreasing NLRP3 inflammasome-loaded exosome release. Conclusions Our findings reveal that ZnONPs induce skin damage in conjunction with UVB exposure. This process involves an interplay of inflammasomes, pyroptosis, autophagy dysfunction, and exosomes in skin toxicity. PT alleviates skin inflammation by regulating the inflammasome–autophagy–exosome pathway, a finding which could prove valuable when further evaluating ZnONPs effects for cosmetic applications.

scholarly journals Zinc oxide nanoparticles augment CD4, CD8, and GLUT-4 expression and restrict inflammation response in streptozotocin-induced diabetic rats

2020 ◽  
Vol 14 (8) ◽  
pp. 680-687
Author(s):  
Norhan Elassy ◽  
Shady El-Dafrawy ◽  
Amira O. Abd El-Azim ◽  
Om Ali Y. El-Khawaga ◽  
Amr Negm
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Diabetic Rats ◽  
Oxide Nanoparticles ◽  
Inflammation Response ◽  
Glut 4

scholarly journals THERAPEUTIC EFFICACY OF ZINC OXIDE NANOPARTICLES IN DIABETIC RATS

2019 ◽  
Vol 56 (22-Suppl) ◽  
Author(s):  
Mohammed Kamal ◽  
Mohamed Khairy ◽  
Nada ELSadek ◽  
Mohamed Hussein
Keyword(s):  
Zinc Oxide ◽  
Therapeutic Efficacy ◽  
Zinc Oxide Nanoparticles ◽  
Diabetic Rats ◽  
Oxide Nanoparticles

scholarly journals Knockdown of KCNQ1OT1 Alleviates the Activation of NLRP3 Inflammasome Through miR-17-5p/TXNIP Axis in Retinal Müller Cells

2021 ◽  
Author(s):  
Yu Liu ◽  
Guoping Cao ◽  
Lili Dong ◽  
Lele Li ◽  
Yuping Dou ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Nlrp3 Inflammasome ◽  
Critical Role ◽  
Müller Cells ◽  
Clinical Samples ◽  
Inflammasome Activation ◽  
Muller Cells ◽  
Potential Mechanism ◽  
Nlrp3 Inflammasome Activation ◽  
Therapeutic Value

Abstract Diabetic retinopathy (DR) is one of the most severe and common complications caused by diabetic mellites. Inhibiting NLRP3 inflammasome activation displays a crucial therapeutic value in DR. Studies have shown that KCNQ1OT1 plays a critical role in regulating NLRP3 inflammasome activation and participates in the pathogenesis of diabetic complications. The present study aims to explore the role, and the potential mechanism of KCNQ1OT1 in regulating the activation of NLRP3 inflammasome in DR. The expression of KCNQ1OT1 and the activation of NLRP3 inflammasome were increased in experimental DR models. KCNQ1OT1 knockdown alleviated NLRP3 inflammasome-associated molecules expression. In addition, KCNQ1OT1 was found to be localized mainly in the cytoplasm of Müller cells and facilitated TXNIP expression by acting as a miR-17-5p sponge. KCNQ1OT1 promoted the activation of NLRP3 inflammasome through miR-17-5p/TXNIP axis. Moreover, the clinical samples of patients with DR showed that the expression of KCNQ1OT1 and the activation of NLRP3 inflammasome were all increased, further supporting the hypothesis that the KCNQ1OT1 dysregulation may be the molecular mechanism of the pathogenesis of DR. Therefore, KCNQ1OT1 may serve as a new therapeutic target for DR.

scholarly journals Ameliorative Effect of Zinc Oxide Nanoparticles on Antioxidants and Sperm Characteristics in Streptozotocin-Induced Diabetic Rat Testes

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Mohamed Afifi ◽  
Omar A. Almaghrabi ◽  
Naif Mohammed Kadasa
Keyword(s):  
Zinc Oxide ◽  
Mrna Expression ◽  
Zinc Oxide Nanoparticles ◽  
Sperm Count ◽  
Testicular Tissue ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Oxide Nanoparticles ◽  
Sperm Characteristics ◽  
The Impact

The present study investigated the impact of zinc oxide nanoparticles (ZnONPs) on the oxidative status and sperm characteristics in diabetic rat testicular tissue. Forty male albino rats were used in this study; 10 of them served as a control and 30 rats were injected with a single dose (100 mg/kg) of streptozotocin intraperitoneally. They were subdivided into diabetic, diabetic + ZnONPs (10 mg/kg B.W.), and diabetic and cotreated with ZnONPs + insulin groups. The sperm count and motility were assessed. The activity and mRNA expression of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GRD), and Glutathion-S-Transferase (GST) were determined in the testicular tissue. Malondialdehyde (MDA) and reduced glutathione (GSH) levels were estimated in the testicular tissue. Sperm count and motility increased in ZnONPs treated diabetic rats. A significant increase in the activity and mRNA expression of SOD, CAT, GPx, GRD, and GST was shown in ZnONPs treated diabetic rats. MDA significantly decreased, while GSH increased in testicular tissue of ZnONPs treated diabetic rats. It was concluded that ZnONPs either alone or in combination with insulin have the ability to increase the sperm count and motility and protect the testicular tissue against the oxidative stress induced by diabetes in rats.

scholarly journals NLRP3 Inflammasome Activation-Mediated Pyroptosis Aggravates Myocardial Ischemia/Reperfusion Injury in Diabetic Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-17 ◽  
Author(s):  
Zhen Qiu ◽  
Shaoqing Lei ◽  
Bo Zhao ◽  
Yang Wu ◽  
Wating Su ◽  
...  
Keyword(s):  
Cell Death ◽  
Myocardial Ischemia ◽  
Nlrp3 Inflammasome ◽  
Ischemia Reperfusion ◽  
Diabetic Rats ◽  
Receptor Protein ◽  
Inflammasome Activation ◽  
Myocardial Infarct Size ◽  
Nlrp3 Inflammasome Activation ◽  
Myocardial Ischemia Reperfusion

The reactive oxygen species- (ROS-) induced nod-like receptor protein-3 (NLRP3) inflammasome triggers sterile inflammatory responses and pyroptosis, which is a proinflammatory form of programmed cell death initiated by the activation of inflammatory caspases. NLRP3 inflammasome activation plays an important role in myocardial ischemia/reperfusion (MI/R) injury. Our present study investigated whether diabetes aggravated MI/R injury through NLRP3 inflammasome-mediated pyroptosis. Type 1 diabetic rat model was established by intraperitoneal injection of streptozotocin (60 mg/kg). MI/R was induced by ligating the left anterior descending artery (LAD) for 30 minutes followed by 2 h reperfusion. H9C2 cardiomyocytes were exposed to high glucose (HG, 30 mM) conditions and hypoxia/reoxygenation (H/R) stimulation. The myocardial infarct size, CK-MB, and LDH release in the diabetic rats subjected to MI/R were significantly higher than those in the nondiabetic rats, accompanied with increased NLRP3 inflammasome activation and increased pyroptosis. Inhibition of inflammasome activation with BAY11-7082 significantly decreased the MI/R injury.In vitrostudies showed similar effects, as BAY11-7082 or the ROS scavenger N-acetylcysteine, attenuated HG and H/R-induced H9C2 cell injury. In conclusion, hyperglycaemia-induced NLRP3 inflammasome activation may be a ROS-dependent process in pyroptotic cell death, and NLRP3 inflammasome-induced pyroptosis aggravates MI/R injury in diabetic rats.

scholarly journals In vivo zinc oxide nanoparticles induces acute hyperglycemic response a dose-dependent and route of administration in healthy and diabetic rats  

2019 ◽  
Author(s):  
Jesús José Ibarra-Leal ◽  
Luis Yocupicio ◽  
Alejandro Apolinar-Iribe ◽  
Irene Díaz-Reval ◽  
Hortensia Parra-Delgado ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Experimental Diabetes ◽  
Intraperitoneal Administration ◽  
Diabetic Rats ◽  
Oxide Nanoparticles ◽  
Acute Effects ◽  
Fasting Glycemia ◽  
Dose Dependent

In recent years, different studies carried out in experimental diabetes models suggest that zinc oxide nanoparticles (ZnONPs) can be good antidiabetic agents, this evidence was obtained from long-term treatments using repeated doses of zinc oxide nanoparticles. The goal of this work was to evaluate the acute effects during six hours post-administration of ZnONPs on glycemia in healthy and diabetic rats and to compare the effects of oral and intraperitoneal administration. For this study male Wistar rats were used and experimental diabetes was induced by streptozotocin-nicotinamide intraperitoneal administration. At short-term ZnONPs administration induced hyperglycemic response in healthy and diabetic rats, the effect was dose-dependent and administration route. The diabetic rats were more sensitive to ZnONPs effect. In conclusion, this study provides novel information about the acute effects of zinc oxide nanoparticles on the fasting glycemia in experimental diabetes in vivo model and healthy rats, these data are important for its future clinical applications.

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