Sodium Acetate, Sodium Acid Pyrophosphate, and Citric Acid Impacts on Isolated Peripheral Lymphocyte Viability, Proliferation, and DNA Damage

2018 ◽  
Vol 32 (8) ◽  
pp. e22171 ◽  
Author(s):  
Yasmina M. Abd-Elhakim ◽  
Abeer Anwar ◽  
Mohamed M. Hashem ◽  
Gihan G. Moustafa ◽  
Khaled Abo-El-Sooud
Keyword(s):  
Dna Damage ◽  
Citric Acid ◽  
Sodium Acetate ◽  
Peripheral Lymphocyte ◽  
Sodium Acid Pyrophosphate

scholarly journals A Study of the Methods of Estimation of Carbohydrates, especially in Plant-extracts: A New Method for the Estimation of Maltose in presence of other Sugars.

1913 ◽  
Vol 5 (4) ◽  
pp. 437-468 ◽  
Author(s):  
William A. Davis ◽  
Arthur John Daish
Keyword(s):  
Citric Acid ◽  
Plant Extracts ◽  
Sodium Acetate ◽  
Cupric Oxide ◽  
Quantitative Estimation ◽  
Gravimetric Method ◽  
Large Error ◽  
Cane Sugar ◽  
Accurate Estimation ◽  
Volumetric Methods

Certain sources of error encountered in the estimation of sugars in plant extracts are dealt with. Large errors in the gravimetric method may be obtained unless special care is taken in purifying the asbestos by boiling for at least 30 minutes with 20% sodium hydroxide. Weighing the reduced copper as cuprous oxide is likely to give rise to large error, and a process of weighing as cupric oxide, with certain precautions, is recommended.The volumetric methods of Ling and of Bertrand have been studied; the former is preferable in all respects to the latter, which we regard as only roughly approximate.In dealing with plant extracts, owing to the accumulation of sodium acetate in the solutions analysed, inversion by citric acid of lower concentration than 10% is generally incomplete. Inversion by invertase is, however, not interfered with by this salt. To estimate cane sugar inversion both by invertase and 10% citric acid is recommended. No loss of sugars occurs owing to the use of basic lead acetate as has been sometimes stated; the supposed loss is probably due to incomplete inversion caused by the presence of sodium acetate.It is shown by a detailed study of the action of dilute hydrochloric acid on different sugars that it is impossible completely to hydrolyse maltose at either 70° or 100° without simultaneously destroying large quantities of laevulose or dextrose.The only available method for the accurate estimation of maltose consists in the employment of special maltase-free yeasts, such as S. exiguus, S. marxiarnus or S. anomalus, introducing a correction (for pentoses, etc.) obtained by a special fermentation with baker's or brewer's yeast.6. A scheme for the quantitative estimation of sugars in plant material is given.

scholarly journals Peripheral lymphocyte DNA damage and oxidative status after eradication therapy in patients infected with Helicobacter pylori

2011 ◽  
Vol 121 (12) ◽  
pp. 428-433
Author(s):  
Ahmet C. Dulger ◽  
Mehmet Aslan ◽  
Yaşar Nazligul ◽  
Mehmet Horoz ◽  
Cengiz Bolukbas ◽  
...  
Keyword(s):  
Dna Damage ◽  
Helicobacter Pylori ◽  
Eradication Therapy ◽  
Oxidative Status ◽  
Peripheral Lymphocyte

scholarly journals Metabolism of Semen After Freezing [[ . The Aerobic Metabolism of Ram Spermatozoa Before and After Storage at ?79°C

1969 ◽  
Vol 22 (3) ◽  
pp. 733
Author(s):  
T O'shea
Keyword(s):  
Citric Acid ◽  
Oxygen Uptake ◽  
Sodium Acetate ◽  
Citric Acid Cycle ◽  
Cell Metabolism ◽  
Sodium Lactate ◽  
Aerobic Metabolism ◽  
Sodium Salts ◽  
Acid Cycle ◽  
Before And After

The metabolism of ram spermatozoa, incubated at 37�0 shortly after ejacula-tion and after storage overnight at -79�0, was examined using various combinations of fructose, sodium lactate, sodium acetate, and sodium salts of citric acid cycle intermediates. Although freezing depressed all indices of cell metabolism, there were few qualitative differences between the metabolism by fresh or thawed semen of the various substrates. The increased oxygen uptake of thawed spermatozoa on addition of succinate was unrelated to motility and to other parameters of metabolism.

ACETATE METABOLISM OF MATURING WHEAT PLANTS

1956 ◽  
Vol 34 (2) ◽  
pp. 180-190 ◽  
Author(s):  
W. B. McConnell ◽  
L. K. Ramachandran
Keyword(s):  
Citric Acid ◽  
Glutamic Acid ◽  
Sodium Acetate ◽  
Citric Acid Cycle ◽  
Starch Synthesis ◽  
Acetate Metabolism ◽  
Labelling Efficiency ◽  
Acid Cycle ◽  
Carbon 14 ◽  
Wheat Kernels

The transport of carbon-14 injected into the hollow stems of growing wheat plants in the form of sodium acetate-1-C14 and -2-C14 was studied. The labelling efficiency of the tracer and its distribution among components of the wheat kernels was markedly dependent upon the time of injection. Maximum incorporation of activity occurred with plants which were given the tracer about 80 days after seeding. Sodium acetate-1-C14 was less effective for producing labelled kernels and gave rise to more uniform distribution of carbon-14 among the components, very little carbon-14 being utilized for starch synthesis nearer maturity. A high percentage of the carbon-14 content of the gluten resided in the glutamic acid residues. Glutamic acid-C14 injected into the stems was an efficient source of labelling for the plant. The results are consistent with the view that acetate is utilized by way of the Krebs' citric acid cycle.

NUTRITIONAL AND METABOLIC STUDIES OF DEBARYOMYCES HANSENII

1962 ◽  
Vol 8 (2) ◽  
pp. 213-220 ◽  
Author(s):  
Emanuel Merdinger ◽  
Salem Shair
Keyword(s):  
Citric Acid ◽  
Sodium Acetate ◽  
Debaryomyces Hansenii ◽  
Chloride Concentration ◽  
Normal Growth ◽  
Nitrogen Sources ◽  
Logarithmic Phase ◽  
Growth Media ◽  
Metabolic Products ◽  
Esterified Sterols

The growth of strain NRRL Y-1448 of Debaryomyces hansenii was studied by using various growth media. A sodium chloride concentration of 2% to 3% and a pH of 5.5 were optimal for growth. No growth occurred below pH 4 and above pH 8. Either thiamine or biotin was required for normal growth. Growth occurred in media containing asparagine plus sodium acetate or asparagine plus citric acid but not citric acid plus acetate. Urea or ammonium sulphate were satisfactory nitrogen sources. The logarithmic phase of growth was reached twice as fast in media containing fructose as in media containing glucose. The metabolic products of growth were alcohol, acetic acid, pyruvic acid, and both free and esterified sterols.

scholarly journals Peripheral lymphocyte DNA damage and oxidative stress in patients with ulcerative colitis

2011 ◽  
Vol 121 (7-8) ◽  
pp. 223-229 ◽  
Author(s):  
Mehmet Aslan ◽  
Yaşar Nazligul ◽  
Cengiz Bolukbas ◽  
Fusun F. Bolukbas ◽  
Mehmet Horoz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ulcerative Colitis ◽  
Dna Damage ◽  
Peripheral Lymphocyte ◽  
And Oxidative Stress

scholarly journals Peripheral Lymphocyte DNA Damage and Oxidative Status in Football Players after a Three-day Football Tournament

2013 ◽  
Vol 52 (2) ◽  
pp. 213-217 ◽  
Author(s):  
Mustafa Atli ◽  
Mehmet Aslan ◽  
Mehmet Emin Kucukoglu ◽  
Haci Bayram Temur ◽  
Abdullah Taskin ◽  
...  
Keyword(s):  
Dna Damage ◽  
Oxidative Status ◽  
Peripheral Lymphocyte ◽  
Football Players

Thermal Inactivation of Conidia from Aspergillus flavus and Aspergillus parasiticus

1975 ◽  
Vol 38 (12) ◽  
pp. 750-758 ◽  
Author(s):  
M. P. DOYLE ◽  
E. H. MARTH
Keyword(s):  
Acetic Acid ◽  
Sodium Chloride ◽  
Citric Acid ◽  
Thermal Resistance ◽  
Aspergillus Flavus ◽  
Sodium Acetate ◽  
Thermal Inactivation ◽  
Aspergillus Parasiticus ◽  
Ph Values ◽  
Strain Dependent

Conidiospores from one strain of Aspergillus flavus and two of Aspergillus parasiticus were thermally inactivated in menstrua at pH values of 3.5, 4.5, 5.5, and 6.0. These values were obtained with the following buffering systems: sodium acetate and acetic acid, citric acid and Na2HPO4, potassium acid phthalate (KHP)-HCl and KHP-NaOH, and KH2PO4 and NaOH. Heating of conidia in a menstruum adjusted to pH 7.0 with KH2PO4 and NaOH served as the control. Use of the sodium acetate and acetic acid buffering system resulted in an increase in the rate at which conidia were inactivated when the pH was decreased. Use of the citric acid and Na2HPO4 buffering system resulted in increased thermal resistance for the conidia as the pH was decreased; however, the degree of increased thermal resistance was strain dependent. When the KHP-HCl and KHP-NaOH buffers were used, conidia were inactivated more rapidly than in the control at the higher pH values and more slowly than in the control at the lower pH values. An increase in amount of sodium chloride, sucrose, or glucose in the menstruum was accompanied by a decrease in the rate at which conidia were inactivated. Generally, sodium chloride was markedly protective to conidia at aw values of less than 0.94, whereas the sugars were markedly protective at values below 0.95. Greatest protection at these values was afforded by sucrose.

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