Antidiabetic potential: in vitro inhibition effects of some natural phenolic compounds on α-glycosidase and α-amylase enzymes

2017 ◽  
Vol 31 (10) ◽  
pp. e21956 ◽  
Author(s):  
Parham Taslimi ◽  
İlhami Gulçin
Keyword(s):  
Phenolic Compounds ◽  
Natural Phenolic Compounds ◽  
In Vitro Inhibition

In Vitro Inhibition of Human Carbonic Anhydrase I and II Isozymes with Natural Phenolic Compounds

2011 ◽  
Vol 77 (6) ◽  
pp. 494-499 ◽  
Author(s):  
Murat Şentürk ◽  
İlhami Gülçin ◽  
Şükrü Beydemir ◽  
Ö. İrfan Küfrevioğlu ◽  
Claudiu T. Supuran
Keyword(s):  
Phenolic Compounds ◽  
Carbonic Anhydrase ◽  
Carbonic Anhydrase I ◽  
Natural Phenolic Compounds ◽  
In Vitro Inhibition ◽  
Human Carbonic Anhydrase

scholarly journals Natural phenolic compounds potentiate hypoglycemia via inhibition of Dipeptidyl peptidase IV

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Po-Kai Huang ◽  
Shian-Ren Lin ◽  
Chia-Hsiang Chang ◽  
May-Jwan Tsai ◽  
Der-Nan Lee ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Blood Sugar ◽  
Dipeptidyl Peptidase ◽  
Dipeptidyl Peptidase Iv ◽  
C2c12 Cells ◽  
Surface Glycoprotein ◽  
Dpp Iv ◽  
Natural Phenolic Compounds

Abstract Dipeptidyl peptidase IV (DPP IV) is a surface glycoprotein that can degrade glucagon like pepetide-1 (GLP-1) by decreasing blood sugar. Herbal medicines for diabetic therapy are widely used with acceptable efficacy but unsatisfied in advances. DPP IV was chosen as a template to employ molecular docking via Discovery Studio to search for natural phenolic compounds whether they have the inhibitory function of DPP IV. Then, docking candidates were validated and further performed signal pathway via Caco-2, C2C12, and AR42J cells. Lastly, a diet-induced diabetes in mice were applied to examine the efficacy and toxicity of hit natural phenolic products in long-term use (in vivo). After screening, curcumin, syringic acid, and resveratrol were found in high affinity with DPP IV enzymes. In enzymatic tests, curcumin and resveratrol showed potential inhibition of DPP IV. In vitro assays, curcumin inhibited of DPP IV activity in Caco-2 cells and ERK phosphorylation in C2C12 cells. Additionally, curcumin attenuated blood sugar in S961-treated C57BL/6 mice and in diet-induced diabetic ICR mice and long-term regulate HbA1c in diabetic mice. Curcumin targeted to DPP IV for reducing blood glucose, it possesses potential and alternative substitution of synthetic clinical drugs for the medication of diabetes.

Molecular Docking of Natural Phenolic Compounds for the Screening of Urease Inhibitors

2019 ◽  
Vol 20 (5) ◽  
pp. 410-421 ◽  
Author(s):  
Ritu Kataria ◽  
Anurag Khatkar
Keyword(s):  
Phenolic Compounds ◽  
In Silico ◽  
Docking Study ◽  
Peptic Ulcers ◽  
Jack Bean Urease ◽  
Jack Bean ◽  
Lead Compounds ◽  
Modelling Techniques ◽  
Natural Phenolic Compounds

Background:Bacterial ureases have been the cause of various human and animal pathogenicity including hepatic encephalopathy, hepatic coma urolithiasis, gastric and peptic ulcers, pyelonephritis, and urinary catheter encrustation by the production of ammonia. Hence, in view of the side effects of existing drugs, there is a strong need to discover, more safe, effective and potent compounds for the treatment of infections caused by urease.Methods:For this purpose, several natural phenolic compounds have been screened by molecular modelling techniques, wherein the phenolic compounds were docked to the active site of Jack bean urease (PDB ID 3LA4) using the Schrodinger docking software.Results:The lead compounds were identified via in-silico screening technique where docking score, binding energy, ADME and toxicity data were considered to screen the lead compounds as compared with the available standard drugs. From the docking study of screened natural phenolic compounds, five compounds diosmin, morin, chlorogenic acid, capsaicin and resveratrol were selected based upon their better affinity towards the receptor and were considered for further wet lab studies.Conclusion:The in-silico results were confirmed by in vitro experiments by use of the Jack bean urease using Weatherburn method.

scholarly journals Activity of Noni Fruit (Morinda citrifolia L.) Ethanolic Extract on Class mu Glutation S-Transferase of Lung Rat

2020 ◽  
Vol 11 (1) ◽  
pp. 46
Author(s):  
Purwanto Purwanto ◽  
Sudibyo Martono
Keyword(s):  
Phenolic Compounds ◽  
Ethanolic Extract ◽  
Morinda Citrifolia ◽  
Glutathione S Transferase ◽  
Fruit Extract ◽  
Effective Response ◽  
Natural Phenolic Compounds ◽  
Gst Activity

One of the main modalities of cancer treatment is chemotherapy, which uses chemicals that are generally electrophilic. These xenobiotic compounds sometimes does not produce effective response due to activity of glutathione S-transferase (GST) which inactivate the xenobiotics. Several natural phenolic compounds were reported to inhibit GST activity in vitro. Noni fruit (Morinda citrifolia L.) which contains flavonoids and other phenolic compounds such as scopoletin and morindon is proposed to interfere GST activity. This study aimed to analyze the effect of ethanolic extract of Noni fruit in vivo on GST activity in lung rat using 1,2-dichloro-4-nitrobenzene (DCNB). This substrate is a specific for class mu GST. First, rats were administered with ethanolic extract of Noni and dimethylbenz(α)anthracene (DMBA) for two weeks. The cytosolic fraction of lung was isolated then the GST activity was determined by simple kinetic program which was automatically calculated using spectrophotometer. The results showed that ethanolic extract of Noni in 1 and 5% (w/v) of concentration induced class mu GST activity, whereas 10% (w/v) of concentration inhibited class mu GST activity. After a treatment with DMBA, all tested concentrations of ethanolic extract of Noni inhibited class mu GST activity of lung rat significantly. These results indicated that Noni fruit extract can be further developed as a supportive agent of a chemotherapy drug.Keywords: DMBA, GST, Morinda citrifolia L., spectrophotometer.

In vitro inhibition of ?-chymotryptic activity by phenolic compounds

2001 ◽  
Vol 81 (15) ◽  
pp. 1512-1521 ◽  
Author(s):  
Sascha Rohn ◽  
Harshadrai M Rawel ◽  
Nadine Pietruschinski ◽  
J�rgen Kroll
Keyword(s):  
Phenolic Compounds ◽  
In Vitro Inhibition ◽  
Chymotryptic Activity

In vitro inhibition of α-carbonic anhydrase isozymes by some phenolic compounds

2011 ◽  
Vol 21 (14) ◽  
pp. 4259-4262 ◽  
Author(s):  
Sevim Beyza Öztürk Sarikaya ◽  
Fevzi Topal ◽  
Murat Şentürk ◽  
İlhami Gülçin ◽  
Claudiu T. Supuran
Keyword(s):  
Phenolic Compounds ◽  
Carbonic Anhydrase ◽  
In Vitro Inhibition
Sign in / Sign up

Export Citation Format

Share Document