New polyoxazoline loaded patches for hemostasis in experimental liver resection

2021 ◽  
Author(s):  
Edwin A. Roozen ◽  
Michiel C. Warlé ◽  
Roger M. L. M. Lomme ◽  
Rosa P. Félix Lanao ◽  
Harry Goor
Keyword(s):  
Liver Resection ◽  
Experimental Liver

Effect of major liver resection on colon cancer metastasis in the lung and liver.

2012 ◽  
Vol 30 (15_suppl) ◽  
pp. e14014-e14014
Author(s):  
Robert M. Hoffman ◽  
Masashi Momiyama ◽  
Atsushi Suetsugu ◽  
Hiroyuki Kishimoto ◽  
Takashi Chishima ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Liver Resection ◽  
Fluorescent Protein ◽  
Metastatic Tumor ◽  
Liver Parenchyma ◽  
Major Liver Resection ◽  
Colon 26 ◽  
Metastatic Growth ◽  
Major Resection ◽  
Experimental Liver

e14014 Background: Liver resection is a common procedure in liver-metastatic colon cancer. However, recurrence is common and distant liver metastasis may be appear after liver resection. Methods: Experimental liver metastases were established by spleen injection of the Colon 26 murine colon cancer cell line expressing green fluorescent protein (GFP) into transgenic nude mice expressing red fluorescent protein (RFP). Experimental liver metastases was established by splenic injection of Colon-26-GFP. Experimental lung metastases were established by tail vein injection with Colon 26-GFP. Three days after cell injection into the spleen or tail vein, groups of mice underwent liver resection (35%+35% [repeated minor resection] vs. 70% [major resection]). Metastatic tumor growth was measured by color-coded fluorescence imaging of the GFP-expressing cancer cells and RFP-expressing stroma. Results: Although major and repeated minor resection removed the same volume of liver parenchyma, the two procedures had very different effects on metastatic tumor growth. Major resection, stimulated liver and lung metastatic growth as well as recruitment of host-derived stroma into the metastases compared to repeated minor resection. Repeated minor resection did not stimulate metastasis or stromal recruitment. There was no significant difference in liver regeneration between the two groups. TGF-β was also preferentially stimulated by major resection. Conclusions: Host-derived stroma density, which is stimulated by major resection compared to repeated minor resection, may stimulate metastatic growth. Stromal growth may be stimulated by TGF-β. The results of this study indicate that caution should be taken when planning a major liver resection for metastatic colorectal cancer. Conservative resections that spare liver parenchyma should be considered whenever possible.

A COMPARISON OF TOPICAL HAEMOSTATIC AGENTS IN EXPERIMENTAL LIVER RESECTION

1987 ◽  
Author(s):  
S D Blaire ◽  
C M Backhouse ◽  
J L Matthews ◽  
C N McCollum
Keyword(s):  
Liver Resection ◽  
Blood Loss ◽  
Calcium Alginate ◽  
Foreign Body Reaction ◽  
Haemostatic Agent ◽  
Haemostatic Agents ◽  
Fibrous Reaction ◽  
Experimental Liver ◽  
Porcine Collagen ◽  
Better Than

Bleeding from lacerated paranchymal organs such as the liver and spleen may be profuse and difficult to control. The haemostatic agent, calcium alginate (Kaltostat), which acts as a source of chelateable calcium ions has been compared with oxidised cellulose (Surgicel) and porcine collagen (Medistat) in experimental liver resection.Under general anaesthesia, a standard 3cm2 piece of liver was excised in 42 NZW rabbits and the haemostatic agent immediately applied. Bleeding was assessed at 3-minute intervals and blood loss precisely measured by haemolysis and a haemoglobinometer. Macroscopic and histological examinations of the liver were performed at 2 and 6 weeks.Calcium alginate stopped bleeding within 3 minutes with minimal blood loss in every animal and was significantly better than the other materials by both criteria. At 2 weeks the macroscopic appearances were similar for the 3 implants with inflammation on microscopy. Oxidised cellulose had absorbed by 6 weeks with calcium alginate having only minimal residual material surrounded by a fibrous reaction. Porcine collagen remained intact with a vigorous foreign body reaction and granuloma formation.Calcium alginate is significantly more effective than the haemostatic materials currently used in surgical practice.

Origin of Hepatic Nuclear Inclusion Bodies

1973 ◽  
Vol 31 ◽  
pp. 426-427
Author(s):  
F. G. Zaki ◽  
J. A. Greenlee ◽  
C. H. Keysser
Keyword(s):  
Inclusion Bodies ◽  
Storage Disease ◽  
Glycogen Storage ◽  
Nutritional Deficiencies ◽  
Nuclear Inclusion ◽  
Electron Microscopic ◽  
Human Liver Cells ◽  
Microscopic Studies ◽  
Per Se ◽  
Experimental Liver

Nuclear inclusion bodies seen in human liver cells may appear in light microscopy as deposits of fat or glycogen resulting from various diseases such as diabetes, hepatitis, cholestasis or glycogen storage disease. These deposits have been also encountered in experimental liver injury and in our animals subjected to nutritional deficiencies, drug intoxication and hepatocarcinogens. Sometimes these deposits fail to demonstrate the presence of fat or glycogen and show PAS negative reaction. Such deposits are considered as viral products.Electron microscopic studies of these nuclei revealed that such inclusion bodies were not products of the nucleus per se but were mere segments of endoplasmic reticulum trapped inside invaginating nuclei (Fig. 1-3).

Liver Resection for Liver Metastasis - the Bernese Results

Swiss Surgery ◽  
2000 ◽  
Vol 6 (4) ◽  
pp. 164-168 ◽  
Author(s):  
Seiler ◽  
Redaelli ◽  
Schmied ◽  
Baer ◽  
Büchler
Keyword(s):  
Liver Resection ◽  
Liver Metastasis ◽  
Chirurgische Resektion ◽  
Kurative Therapie

Neue Erkenntnisse über die Anatomie und Funktion der Leber haben dazu geführt, dass heute die chirurgische Resektion die Therapie der Wahl bei Lebermetastasen geworden ist. Obschon Lebermetastasen ein fortgeschrittenes Tumorstadium bedeuten, werden infolge besserer Kenntnisse der Karzinogenese (Mikrometastasen etc.) sowie der prognostischen Risikofaktoren erwiesenermassen die besten Langzeitresultate durch die chirurgische Resektion erzielt. In dieser Studie wurden die Ergebnisse von 109 Resektionen von kolorektalen sowie nicht kolorektalen Lebermetastasen an unserer Klinik während eines Zeitraumes von 59 Monaten zusammengefasst. Vier verschiedene Operationsverfahren (formelle Hemihepatektomie vs Segmentresektion vs atypische Resektion vs Biopsie) wurden untersucht. Die Einhaltung eines Resektionsabstandes von mindestens 10 mm wurde bei Resektionen immer angestrebt. Die kumulierte Morbidität aller Operationsverfahren zusammen betrug 23%. Obwohl die Morbidität bei ausgedehnten Resektionen höher war (Encephalopathie 16% vs 2.3% bei der Segmentresektion, Leberinsuffizienz 23% vs 4.7%), war das Langzeitüberleben gegenüber den limitierten Resektionsverfahren verbessert. Die 60-Tage Mortalität lag bei 2.7%. Patienten nach Resektion von kolorektalen Lebermetastasen hatten eine höhere Ueberlebensrate als diejenigen nach Resektion nicht kolorektaler Metastasen. Unsere Resultate zeigen, dass die Leberresektion heutzutage unter Einhaltung der anatomischen sowie funktionellen Grenzen (inkl. eines adäquaten Resektionsrandes) die einzige, potentiell kurative Therapie von Lebermetastasen darstellt. Trotz erhöhter perioperativer Morbidität ist die ausgedehnte formelle Resektion den limitierten Operationsverfahren bezüglich Langzeitüberleben überlegen. Ein Grund dafür ist die erhöhte Wahrscheinlichkeit einer Mitresektion von präoperativ nicht detektierbaren lokalen Mikrometastasen.

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