Synthesis and in vivo evaluation of a scaffold containing wollastonite/β‐TCP for bone repair in a rabbit tibial defect model

2019 ◽  
Vol 108 (3) ◽  
pp. 1107-1116 ◽  
Author(s):  
Willams T. Barbosa ◽  
Katilayne V. Almeida ◽  
Gabriel G. Lima ◽  
Miguel A. Rodriguez ◽  
Marcos V. Lia Fook ◽  
...  
Keyword(s):  
Bone Repair ◽  
Defect Model ◽  
In Vivo Evaluation ◽  
Tibial Defect

In vivo evaluation of resorbable bone graft substitutes in a rabbit tibial defect model

Biomaterials ◽  
2004 ◽  
Vol 25 (20) ◽  
pp. 5037-5044 ◽  
Author(s):  
D. Stubbs ◽  
M. Deakin ◽  
P. Chapman-Sheath ◽  
W. Bruce ◽  
J. Debes ◽  
...  
Keyword(s):  
Bone Graft ◽  
Defect Model ◽  
In Vivo Evaluation ◽  
Tibial Defect ◽  
Bone Graft Substitutes

Zirconium modified calcium‐silicate‐based nanoceramics: An in vivo evaluation in a rabbit tibial defect model

2018 ◽  
Vol 16 (2) ◽  
pp. 431-437 ◽  
Author(s):  
Ali Doostmohammadi ◽  
Zahra Karimzadeh Esfahani ◽  
Abdolreza Ardeshirylajimi ◽  
Zahra Rahmati Dehkordi
Keyword(s):  
Calcium Silicate ◽  
Defect Model ◽  
In Vivo Evaluation ◽  
Tibial Defect

Fabrication of hybrid scaffolds by polymer deposition system and its in-vivo evaluation with a rat tibial defect model

2014 ◽  
Vol 11 (6) ◽  
pp. 439-445 ◽  
Author(s):  
Min-Woo Sa ◽  
Sung Eun Kim ◽  
Young-Pil Yun ◽  
Hae-Ryong Song ◽  
Jong Young Kim
Keyword(s):  
Defect Model ◽  
In Vivo Evaluation ◽  
Tibial Defect ◽  
Polymer Deposition ◽  
Hybrid Scaffolds

scholarly journals In Vivo Evaluation of 3D-Printed Polycaprolactone Scaffold Implantation Combined with β-TCP Powder for Alveolar Bone Augmentation in a Beagle Defect Model

Materials ◽  
2018 ◽  
Vol 11 (2) ◽  
pp. 238 ◽  
Author(s):  
Su Park ◽  
Hyo-Jung Lee ◽  
Keun-Suh Kim ◽  
Sang Lee ◽  
Jung-Tae Lee ◽  
...  
Keyword(s):  
Alveolar Bone ◽  
Bone Augmentation ◽  
Defect Model ◽  
In Vivo Evaluation ◽  
3D Printed

In vivo evaluation of interactions between biphasic calcium phosphate (BCP)-niobium pentoxide (Nb2O5) nanocomposite and tissues using a rat critical-size calvarial defect model

2020 ◽  
Vol 31 (8) ◽  
Author(s):  
Helio de Jesus Kiyochi Junior ◽  
Aline Gabriela Candido ◽  
Taiana Gabriela Moretti Bonadio ◽  
José Adauto da Cruz ◽  
Mauro Luciano Baesso ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Biphasic Calcium Phosphate ◽  
Critical Size ◽  
Niobium Pentoxide ◽  
Calvarial Defect ◽  
Defect Model ◽  
In Vivo Evaluation

scholarly journals In Vitro and In Vivo Evaluation of Commercially Available Fibrin Gel as a Carrier of Alendronate for Bone Tissue Engineering

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Beom Su Kim ◽  
Feride Shkembi ◽  
Jun Lee
Keyword(s):  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Release Kinetics ◽  
Human Mesenchymal Stem Cells ◽  
Calvarial Defect ◽  
Defect Model ◽  
Fibrin Gel ◽  
In Vivo Evaluation

Alendronate (ALN) is a bisphosphonate drug that is widely used for the treatment of osteoporosis. Furthermore, local delivery of ALN has the potential to improve the bone regeneration. This study was designed to investigate an ALN-containing fibrin (fibrin/ALN) gel and evaluate the effect of this gel on both in vitro cellular behavior using human mesenchymal stem cells (hMSCs) and in vivo bone regenerative capacity. Fibrin hydrogels were fabricated using various ALN concentrations (10−7–10−4 M) with fibrin glue and the morphology, mechanical properties, and ALN release kinetics were characterized. Proliferation and osteogenic differentiation of and cytotoxicity in fibrin/ALN gel-embedded hMSCs were examined. In vivo bone formation was evaluated using a rabbit calvarial defect model. The fabricated fibrin/ALN gel was transparent with Young’s modulus of ~13 kPa, and these properties were not affected by ALN concentration. The in vitro studies showed sustained release of ALN from the fibrin gel and revealed that hMSCs cultured in fibrin/ALN gel showed significantly increased proliferation and osteogenic differentiation. In addition, microcomputed tomography and histological analysis revealed that the newly formed bone was significantly enhanced by implantation of fibrin/ALN gel in a calvarial defect model. These results suggest that fibrin/ALN has the potential to improve bone regeneration.

IN VIVO EVALUATION OF A NEW β-TRICALCIUM PHOSPHATE BONE SUBSTITUTE IN A RABBIT FEMUR DEFECT MODEL

2015 ◽  
Vol 27 (03) ◽  
pp. 1550028 ◽  
Author(s):  
Kam-Kong Chan ◽  
Chia-Hsien Chen ◽  
Lien-Chen Wu ◽  
Yi-Jie Kuo ◽  
Chun-Jen Liao ◽  
...  
Keyword(s):  
Bone Graft ◽  
Tricalcium Phosphate ◽  
Bone Substitute ◽  
Zealand White Rabbit ◽  
Bone Substitutes ◽  
Bone Graft Substitute ◽  
Defect Model ◽  
In Vivo Evaluation ◽  
Rabbit Femur

Calcium phosphate ceramics, of a similar composition to that of mineral bone, and which possess the properties of bioactivity and osteoconductivity, have been widely used as substitutes for bone graft in orthopedic, plastic and craniofacial surgeries. A synthetic β-tricalcium phosphate, Osteocera™, a recently developed bone graft substitute, has been used in this study. To evaluate the affinity and efficacy of Osteocera™ as bone defect implant, we used a New Zealand white rabbit femur defect model to test the osteoconductivity of this new bone substitute. Alternative commercially available bone substitutes, Triosite® and ProOsteon500, were used as the control materials. These three bone substitutes show good biocompatibility, and no abnormal inflammation either infection was seen at the implantation sites. In the histological and histomorphometric images, newly formed bone grew into the peripheral pores in the bone substitutes. After six months implantation, the volume of bone formation was found to be 20.5 ± 5.2%, 29.8 ± 6.5% and 75.5 ± 4.9% of the potential total cavity offered by ProOsteon500, Triosite® and Osteocera™, respectively. The newly formed bone area within the femur defect section for Osteocera™ was significantly larger than ProOsteon500 and Triosite®. We concluded that Osteocera™ shows better bioresorbability, biocompatibility and osteoconductivity in the rabbit femur defect model.

IN VIVO EVALUATION OF BOVINE XENOGRAFT ASSOCIATED WITH OXYGEN THERAPY IN ALVEOLAR BONE REPAIR

2020 ◽  
Author(s):  
Camila Saggioro ◽  
Suelen Sartoretto ◽  
Isabelle Duarte ◽  
Adriana Alves ◽  
Helder Barreto ◽  
...  
Keyword(s):  
Bone Repair ◽  
Alveolar Bone ◽  
Healing Process ◽  
Active Oxygen ◽  
Control Group ◽  
Wound Healing Process ◽  
Bovine Bone ◽  
In Vivo Evaluation ◽  
Significant Difference

In order to preserve alveolar bone thickness and width after extraction, clinical strategies have been adopted to reduce or eliminate the need for future surgical interventions to increase the alveolar ridge. The use of xenogeneic biomaterials has been increasing for such application. The association of bone substitutes with active oxygen-based materials, which is essential in the wound healing process, could accelerate the bone repair, optimizing the maintenance of alveolar architecture after extraction. However, the truth of this hypothesis is not clear. The present study aimed to compare the biological response to inorganic bovine bone graft Bonefill® (BF), associated or not with active oxygen-based gel Oral gel Blue ® m (BF+BM), in alveolar bone repair. Twenty female Wistar rats were randomly distributed, the left upper central incisor was extracted and the dental sockets were filled with BF in the control group (n=10), and with BF+BM in the experimental group (n=10). After 7- and 42-days’ post implantation (n=5), the animals were euthanized, and the samples were processed for descriptive histological and histomorphometric evaluations. The results showed no significant difference between the groups (p>0.05). Both groups presented a time-dependent increase of new formed bone and biosorption biomaterial (p=0.0001). While the biomaterial analyzed was considered compatible and osteoconductive, the association with Blue ® m gel did not improve or interfere with the bone repair after the experimental periods.

scholarly journals In Vitro Prevascularization of Self-Assembled Human Bone-Like Tissues and Preclinical Assessment Using a Rat Calvarial Bone Defect Model

Materials ◽  
2021 ◽  
Vol 14 (8) ◽  
pp. 2023
Author(s):  
Fabien Kawecki ◽  
Todd Galbraith ◽  
William P. Clafshenkel ◽  
Michel Fortin ◽  
François A. Auger ◽  
...  
Keyword(s):  
Bone Defect ◽  
Bone Repair ◽  
Human Bone ◽  
Male Rats ◽  
Defect Model ◽  
Calvarial Bone ◽  
Calvarial Bone Defect ◽  
Self Assembled

In vitro prevascularization has the potential to address the challenge of maintaining cell viability at the core of engineered constructs, such as bone substitutes, and to improve the survival of tissue grafts by allowing quicker anastomosis to the host microvasculature. The self-assembly approach of tissue engineering allows the production of biomimetic bone-like tissue constructs including extracellular matrix and living human adipose-derived stromal/stem cells (hASCs) induced towards osteogenic differentiation. We hypothesized that the addition of endothelial cells could improve osteogenesis and biomineralization during the production of self-assembled human bone-like tissues using hASCs. Additionally, we postulated that these prevascularized constructs would consequently improve graft survival and bone repair of rat calvarial bone defects. This study shows that a dense capillary network spontaneously formed in vitro during tissue biofabrication after two weeks of maturation. Despite reductions in osteocalcin levels and hydroxyapatite formation in vitro in prevascularized bone-like tissues (35 days of culture), in vivo imaging of prevascularized constructs showed an improvement in cell survival without impeding bone healing after 12 weeks of implantation in a calvarial bone defect model (immunocompromised male rats), compared to their stromal counterparts. Globally, these findings establish our ability to engineer prevascularized bone-like tissues with improved functional properties.

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