Biodegradable cell‐laden starch foams for the rapid fabrication of 3D tissue constructs and the application in neural tissue engineering

2019 ◽  
Vol 108 (1) ◽  
pp. 104-116 ◽  
Author(s):  
Xiaoxiao Wen ◽  
Minjie Shen ◽  
Yanjie Bai ◽  
Changlu Xu ◽  
Xinglong Han ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Neural Tissue ◽  
Neural Tissue Engineering ◽  
Tissue Constructs ◽  
Rapid Fabrication ◽  
Starch Foams

scholarly journals Physical and Mechanical Characterization of Fibrin-Based Bioprinted Constructs Containing Drug-Releasing Microspheres for Neural Tissue Engineering Applications

Processes ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 1205
Author(s):  
Ruchi Sharma ◽  
Rebecca Kirsch ◽  
Karolina Papera Valente ◽  
Milena Restan Perez ◽  
Stephanie Michelle Willerth
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Loss Modulus ◽  
Physical And Mechanical Properties ◽  
Neural Tissue ◽  
Neuronal Cultures ◽  
Neural Cells ◽  
Neural Tissue Engineering ◽  
Mechanical Reinforcement ◽  
Tissue Constructs

Three-dimensional bioprinting can fabricate precisely controlled 3D tissue constructs. This process uses bioinks—specially tailored materials that support the survival of incorporated cells—to produce tissue constructs. The properties of bioinks, such as stiffness and porosity, should mimic those found in desired tissues to support specialized cell types. Previous studies by our group validated soft substrates for neuronal cultures using neural cells derived from human-induced pluripotent stem cells (hiPSCs). It is important to confirm that these bioprinted tissues possess mechanical properties similar to native neural tissues. Here, we assessed the physical and mechanical properties of bioprinted constructs generated from our novel microsphere containing bioink. We measured the elastic moduli of bioprinted constructs with and without microspheres using a modified Hertz model. The storage and loss modulus, viscosity, and shear rates were also measured. Physical properties such as microstructure, porosity, swelling, and biodegradability were also analyzed. Our results showed that the elastic modulus of constructs with microspheres was 1032 ± 59.7 Pascal (Pa), and without microspheres was 728 ± 47.6 Pa. Mechanical strength and printability were significantly enhanced with the addition of microspheres. Thus, incorporating microspheres provides mechanical reinforcement, which indicates their suitability for future applications in neural tissue engineering.

scholarly journals Spidroin Silk Fibers with Bioactive Motifs of Extracellular Proteins for Neural Tissue Engineering

ACS Omega ◽  
2021 ◽  
Author(s):  
Veronica A. Revkova ◽  
Konstantin V. Sidoruk ◽  
Vladimir A. Kalsin ◽  
Pavel A. Melnikov ◽  
Mikhail A. Konoplyannikov ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Neural Tissue ◽  
Extracellular Proteins ◽  
Neural Tissue Engineering ◽  
Silk Fibers

scholarly journals Electrospun Nanofibrous Materials for Neural Tissue Engineering

Polymers ◽  
2011 ◽  
Vol 3 (1) ◽  
pp. 413-426 ◽  
Author(s):  
Yee-Shuan Lee ◽  
Treena Livingston Arinzeh
Keyword(s):  
Tissue Engineering ◽  
Neural Tissue ◽  
Neural Tissue Engineering

Directing Axonal Growth: A Review on the Fabrication of Fibrous Scaffolds That Promotes the Orientation of Axons

Gels ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 25
Author(s):  
Devindraan Sirkkunan ◽  
Belinda Pingguan-Murphy ◽  
Farina Muhamad
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Neuronal Cells ◽  
Neural Tissue ◽  
Axonal Growth ◽  
Structural Proteins ◽  
Neural Cells ◽  
Neural Tissue Engineering ◽  
Fibrous Scaffolds ◽  
Specialized Function

Tissues are commonly defined as groups of cells that have similar structure and uniformly perform a specialized function. A lesser-known fact is that the placement of these cells within these tissues plays an important role in executing its functions, especially for neuronal cells. Hence, the design of a functional neural scaffold has to mirror these cell organizations, which are brought about by the configuration of natural extracellular matrix (ECM) structural proteins. In this review, we will briefly discuss the various characteristics considered when making neural scaffolds. We will then focus on the cellular orientation and axonal alignment of neural cells within their ECM and elaborate on the mechanisms involved in this process. A better understanding of these mechanisms could shed more light onto the rationale of fabricating the scaffolds for this specific functionality. Finally, we will discuss the scaffolds used in neural tissue engineering (NTE) and the methods used to fabricate these well-defined constructs.

scholarly journals Electrospun Polyvinylidene Fluoride-Based Fibrous Scaffolds with Piezoelectric Characteristics for Bone and Neural Tissue Engineering

Nanomaterials ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 952 ◽  
Author(s):  
Li ◽  
Liao ◽  
Tjong
Keyword(s):  
Tissue Engineering ◽  
Polyvinylidene Fluoride ◽  
Neural Tissue ◽  
Electrospinning Process ◽  
Neural Cells ◽  
Neural Tissue Engineering ◽  
Engineering Applications ◽  
Pore Sizes ◽  
Β Phase ◽  
Small Pore

Polyvinylidene fluoride (PVDF) and polyvinylidene fluoride-trifluoroethylene (P(VDF-TrFE) with excellent piezoelectricity and good biocompatibility are attractive materials for making functional scaffolds for bone and neural tissue engineering applications. Electrospun PVDF and P(VDF-TrFE) scaffolds can produce electrical charges during mechanical deformation, which can provide necessary stimulation for repairing bone defects and damaged nerve cells. As such, these fibrous mats promote the adhesion, proliferation and differentiation of bone and neural cells on their surfaces. Furthermore, aligned PVDF and P(VDF-TrFE) fibrous mats can enhance neurite growth along the fiber orientation direction. These beneficial effects derive from the formation of electroactive, polar β-phase having piezoelectric properties. Polar β-phase can be induced in the PVDF fibers as a result of the polymer jet stretching and electrical poling during electrospinning. Moreover, the incorporation of TrFE monomer into PVDF can stabilize the β-phase without mechanical stretching or electrical poling. The main drawbacks of electrospinning process for making piezoelectric PVDF-based scaffolds are their small pore sizes and the use of highly toxic organic solvents. The small pore sizes prevent the infiltration of bone and neuronal cells into the scaffolds, leading to the formation of a single cell layer on the scaffold surfaces. Accordingly, modified electrospinning methods such as melt-electrospinning and near-field electrospinning have been explored by the researchers to tackle this issue. This article reviews recent development strategies, achievements and major challenges of electrospun PVDF and P(VDF-TrFE) scaffolds for tissue engineering applications.

Nanotechnologies in Neural Tissue Engineering

2008 ◽  
pp. 295-309
Author(s):  
Julie Yeh ◽  
Vivek Mukhatyar ◽  
Ravi Bellamkonda
Keyword(s):  
Tissue Engineering ◽  
Neural Tissue ◽  
Neural Tissue Engineering

scholarly journals The Application of Alginate Scaffold in Neural Tissue Engineering

2017 ◽  
Vol 5 (4) ◽  
pp. 76-86 ◽  
Author(s):  
Maryam Borhani-Haghighi ◽  
Shahnaz Razavi ◽  
Zahra Khosravizadeh ◽  
◽  
◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Neural Tissue ◽  
Neural Tissue Engineering ◽  
Alginate Scaffold

scholarly journals Microfluidic systems for stem cell-based neural tissue engineering

Lab on a Chip ◽  
2016 ◽  
Vol 16 (14) ◽  
pp. 2551-2571 ◽  
Author(s):  
Mahdi Karimi ◽  
Sajad Bahrami ◽  
Hamed Mirshekari ◽  
Seyed Masoud Moosavi Basri ◽  
Amirala Bakhshian Nik ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Stem Cell ◽  
Neural Tissue ◽  
Microfluidic Systems ◽  
Neural Tissue Engineering ◽  
Stem Cell Culture ◽  
Stem Cell Derivation

Overall process of stem cell derivation and isolation, as well as microfluidic stem cell culture and neural tissue engineering.

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