A microelectrode array chip for osteogenic differentiation of mesenchymal stem cells under electrical stimulation

Lab on a Chip ◽  
2020 ◽  
Vol 20 (2) ◽  
pp. 373-383 ◽  
Author(s):  
Tianyang Zheng ◽  
Zhizhong Zhang ◽  
Rong Zhu ◽  
Dong Sun
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Electrical Stimulation ◽  
Electric Field ◽  
Osteogenic Differentiation ◽  
Microelectrode Array ◽  
Uniform Electric Field

A microelectrode array chip is able to improve the osteogenic differentiation of mesenchymal stem cells by generating a non-uniform electric field.

scholarly journals In vitroeffect of direct current electrical stimulation on rat mesenchymal stem cells

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e2821 ◽  
Author(s):  
Sahba Mobini ◽  
Liudmila Leppik ◽  
Vishnu Thottakkattumana Parameswaran ◽  
John Howard Barker
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Electrical Stimulation ◽  
Osteogenic Differentiation ◽  
Direct Current ◽  
Calcium Deposition ◽  
Time Points ◽  
Rat Bone

BackgroundElectrical stimulation (ES) has been successfully used to treat bone defects clinically. Recently, both cellular and molecular approaches have demonstrated that ES can change cell behavior such as migration, proliferation and differentiation.MethodsIn the present study we exposed rat bone marrow- (BM-) and adipose tissue- (AT-) derived mesenchymal stem cells (MSCs) to direct current electrical stimulation (DC ES) and assessed temporal changes in osteogenic differentiation. We applied 100 mV/mm of DC ES for 1 h per day for three, seven and 14 days to cells cultivated in osteogenic differentiation medium and assessed viability and calcium deposition at the different time points. In addition, expression of osteogenic genes, Runx2, Osteopontin, and Col1A2 was assessed in BM- and AT-derived MSCs at the different time points.ResultsResults showed that ES changed osteogenic gene expression patterns in both BM- and AT-MSCs, and these changes differed between the two groups. In BM-MSCs, ES caused a significant increase in mRNA levels of Runx2, Osteopontin and Col1A2 at day 7, while in AT-MSCs, the increase in Runx2 and Osteopontin expression were observed after 14 days of ES.DiscussionThis study shows that rat bone marrow- and adipose tissue-derived stem cells react differently to electrical stimuli, an observation that could be important for application of electrical stimulation in tissue engineering.

BMP9 Regulates Osteogenic Differentiation of Mesenchymal Stem Cells Through JNKs Kinase Pathway

2013 ◽  
Vol 40 (12) ◽  
pp. 1220
Author(s):  
Jing XU ◽  
Dan ZHAO ◽  
Jian WANG ◽  
WenJuan WANG ◽  
JinYong LUO
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Kinase Pathway

Wnt7a Promotes Osteogenic Differentiation of Human Mesenchymal Stem Cells

2019 ◽  
Author(s):  
Leiluo Yang ◽  
Qing Li ◽  
Junhong Zhang ◽  
Pengcheng Li ◽  
Chaoliang Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Human Mesenchymal Stem Cells
Sign in / Sign up

Export Citation Format

Share Document