In vitro cytotoxicity of galvanically coupled magnesium‐titanium particles on human osteosarcoma SAOS2 cells: A potential cancer therapy

2018 ◽  
Vol 107 (1) ◽  
pp. 178-189 ◽  
Author(s):  
Jua Kim ◽  
Jeremy L. Gilbert
Keyword(s):  
Cancer Therapy ◽  
In Vitro Cytotoxicity ◽  
Human Osteosarcoma ◽  
Potential Cancer

scholarly journals A COMPARATIVE ANTICANCER STUDY OF BIOSYNTHESIZED NANOPARTICLES, DOXORUBICIN & NANO-DOX CONJUGATE AGAINST CERVICAL CANCER HELA CELL LINE

2020 ◽  
pp. 4-7
Author(s):  
M. R. Kamala Priya ◽  
Priya R. Iyer
Keyword(s):  
Cervical Cancer ◽  
Gold Nanoparticles ◽  
Cell Death ◽  
Cancer Therapy ◽  
Hela Cell ◽  
Cell Line ◽  
In Vitro Cytotoxicity ◽  
Hela Cell Line ◽  
Vero Cell Line

Doxorubicin is the most common chemotherapy drug used in cancer therapy. Its usage is associated with various side-effects. In order to overcome the challenges in Doxorubicin administration, the present study has focussed on synthesizing a drug conjugate with biosynthesized gold nanoparticles and doxorubicin. The gold nanoparticles were biosynthesized using green extracts of medicinal plants with potential anticancer activities. The nanoparticle that possesses anticancer activity was conjugated with the drug for a combinatorial effect of the nanoparticles and the drug. The in vitro cytotoxicity was checked in Vero cell line through MTT assay. The in vitro anti proliferative effects were screened against cervical cancer in HeLa cell line. Fluorescence activated cell sorting analysis was carried out to detect the difference between live and dead cell populations. The preliminary confirmation was carried out in UV-VIS spectrophotometer. The morphological characterization was carried out by SEM and stability by Zeta potential. The IC50 of the nanocompounds demonstrated anti-proliferative activity against cervical cancer similar to the chemotherapeutic drug, Doxorubicin; additionally in a much lesser concentration of the drug. The in vitro cytotoxicity exhibited high viability of cells in Vero cell line with minimum viability of 80% in all the tested concentrations. There was a synergistic effect of the nanoparticles along with the drug; thereby an enhanced therapeutic efficiency was achieved. FACS analysis showed 36% of cell death in Dox treated HeLa cells whereas 96% of cell death in Nano-Dox treated HeLa cells. Nano-Dox conjugate has demonstrated high anticancer effects than drug alone Doxorubicin. Further biosynthesized nanomaterials based drug formulation can be developed as a potential strategy in cancer therapy.

Physicochemical and in vitro cytotoxicity evaluation of polymeric drugs for combination cancer therapy

2019 ◽  
Vol 69 (17) ◽  
pp. 1134-1148 ◽  
Author(s):  
Blessing Atim Aderibigbe ◽  
Tobeka Naki ◽  
Vanessa Steenkamp ◽  
Mutshinyalo Nwamadi ◽  
Suprakas Sinha Ray ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
In Vitro Cytotoxicity ◽  
Polymeric Drugs ◽  
Combination Cancer Therapy

Towards the realization of luminescence from visible emitting trivalent lanthanides (Sm, Eu, Tb, Dy) in polar zinc sulfide nanoparticles: evaluation of in vitro cytotoxicity

RSC Advances ◽  
2016 ◽  
Vol 6 (49) ◽  
pp. 43304-43315 ◽  
Author(s):  
Arijita Chakraborty ◽  
Gouranga H. Debnath ◽  
Manisha Ahir ◽  
Saurav Bhattacharya ◽  
Priyanka Upadhyay ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Zinc Sulfide ◽  
Cellular Imaging ◽  
In Vitro Cytotoxicity ◽  
Trivalent Lanthanides ◽  
Water Dispersible ◽  
Anti Cancer ◽  
Zinc Sulfide Nanoparticles

This study develops water dispersible trivalent terbium cation incorporated zinc sulfide nanoparticles for potential anti-cancer therapy and cellular imaging.

scholarly journals Ionically Crosslinked Chitosan Membranes Used as Drug Carriers for Cancer Therapy Application

Materials ◽  
2018 ◽  
Vol 11 (10) ◽  
pp. 2051 ◽  
Author(s):  
Alecsandra Ferreira Tomaz ◽  
Sandra Sobral de Carvalho ◽  
Rossemberg Cardoso Barbosa ◽  
Suédina L. Silva ◽  
Marcos Sabino Gutierrez ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Drug Release ◽  
Delivery System ◽  
Drug Carriers ◽  
In Vitro Cytotoxicity ◽  
Cytotoxicity Studies ◽  
Chitosan Membranes ◽  
Crosslinked Chitosan ◽  
Cross Linker

The aim of this paper was to prepare, by the freeze-drying method, ionically crosslinked chitosan membranes with different contents of pentasodium tripolyphosphate (TPP) and loaded with 1,4-naphthoquinone (NQ14) drug, in order to evaluate how the physical crosslinking affects NQ14 release from chitosan membranes for cancer therapy application. The membranes were characterized by Fourier transform infrared spectroscopy (FTIR), wide-angle X-ray diffraction (WAXD), scanning electron microscopy (SEM), swelling degree, and through in vitro drug release and cytotoxicity studies. According to the results, the molecular structure, porosity and hydrophilicity of the chitosan membranes were affected by TPP concentration and, consequently, the NQ14 drug release behavior from the membranes was also affected. The release of NQ14 from crosslinked chitosan membranes decreased when the cross-linker TPP quantity increased. Thus, depending on the TPP amount, the crosslinked chitosan membranes would be a potential delivery system to control the release of NQ14 for cancer therapy application. Lastly, the inhibitory potential of chitosan membranes ionically crosslinked with TPP and loaded with NQ14 against the B16F10 melanoma cell line was confirmed through in vitro cytotoxicity studies assessed via MTT assay. The anti-proliferative effect of prepared membranes was directly related to the amount of cross-linker and among all membranes prepared, such that one crosslinked with 0.3% of TPP may become a potential delivery system for releasing NQ14 drug for cancer therapy.

Cisplatin-loaded Au–Au2S nanoparticles for potential cancer therapy: Cytotoxicity,in vitro carcinogenicity, and cellular uptake

2008 ◽  
Vol 85A (3) ◽  
pp. 787-796 ◽  
Author(s):  
Lei Ren ◽  
Xiao-Li Huang ◽  
Bin Zhang ◽  
Li-Ping Sun ◽  
Qi-Qing Zhang ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Cellular Uptake ◽  
Potential Cancer

scholarly journals A Redox-Sensitive Micelle-Like Nanoparticle Self-Assembled from Amphiphilic Adriamycin-Human Serum Albumin Conjugates for Tumor Targeted Therapy

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Lin Chen ◽  
Feng Chen ◽  
Mengxin Zhao ◽  
Xiandi Zhu ◽  
Changhong Ke ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Disulfide Bond ◽  
Imaging System ◽  
In Vitro Cytotoxicity ◽  
Gastric Cancer Cells

The application of chemotherapeutic drug adriamycin (ADR) in cancer therapy is limited by its side effects like high toxicity and insolubility. Nanomedicine offers new hope for overcoming the shortcomings. But how to increase in vivo stability and to control intracellular drug release is a key issue for nano-based formulations. Herein, the hydrophobic ADR was successfully linked to the biocompatible human serum albumin (HSA) by disulfide bond 3-(2-pyridyldithio) propionyl hydrazide (PDPH), resulting in amphiphilic HSA-ADR. The novel ADR-HSA micellar NPs which were thus assembled exhibited a well-defined stable core shell structure with glutathione (GSH) sensitive linkers. The stable PDPH linkers at extracellular level were broken by GSH at intracellular level with a controlled ADR release profile. The in vitro cytotoxicity against gastric cancer cells (NCI-N87) was obviously enhanced by such redox-sensitive ADR-HSA NPs. Additionally, as observed by IVIS Lumina II Imaging System (Xenogen), the intratumor accumulation of ADR-HSA NPs was much higher than that of HSA/ADR NPs due to its high stability. Consequently, the in vivo tumor inhibition was significantly promoted after intravenous administration to the Balb/c nude mice bearing gastric tumors. These in vitro/vivo results indicated that disulfide-bond-containing ADR-HSA NPs were an effective nanodrug delivery system for cancer therapy.

In Vitro Cytotoxicity of Binary Ti Alloys for Bone Implants

2009 ◽  
Vol 618-619 ◽  
pp. 295-298 ◽  
Author(s):  
Yun Cang Li ◽  
Cynthia Wong ◽  
Jian Yu Xiong ◽  
Peter D. Hodgson ◽  
Cui E Wen
Keyword(s):  
Weight Ratio ◽  
High Strength ◽  
In Vitro Cytotoxicity ◽  
Osteosarcoma Cell ◽  
Human Osteosarcoma Cell Line ◽  
Human Osteosarcoma ◽  
Ti Alloys ◽  
Human Osteosarcoma Cell ◽  
Cell Shapes

Interest in using titanium (Ti) alloys as load-bearing implant materials has increased due to their high strength to weight ratio, lower elastic modulus, and superior biocompatibility and enhanced corrosion resistance compared to conventional metals such as stainless steel and Co-Cr alloys. In the present study, the in vitro cytotoxicity of five binary titanium alloys, Ti15Ta, Ti15Nb, Ti15Zr, Ti15Sn and Ti15Mo, was assessed using human osteosarcoma cell line, SaOS-2 cells. The Cell proliferation and viability were determined, and cell adhesion and morphology on the surfaces of the binary Ti alloys after cell culture were observed by SEM. Results indicated that the Ti binary alloys of Ti15Ta, Ti15Nb and Ti15Zr exhibited the same level of excellent biocompatibility; Ti15Sn alloy exhibited a moderate biocompatibility while Ti15Mo alloy exhibited a moderate cytotoxicity. The SaOS-2 osteoblast-like cells had flattened and spread across the surfaces of the Ti15Ta, Ti15Nb, Ti15Zr and Ti15Sn groups; however, the cell shapes on the Ti15Mo alloy was shrinking and unhealthy. These results indicated that the Mo contents should be limited to a certain level in the design and development of new Ti alloys for implant material applications.

In vitro Cytotoxicity and Genotoxicity Analysis of Ten Tannery Chemicals Using SOS/umu Tests and High-content In vitro Micronucleus Tests

2018 ◽  
Vol 21 (4) ◽  
pp. 262-270 ◽  
Author(s):  
Zehao Huang ◽  
Na Li ◽  
Kaifeng Rao ◽  
Cuiting Liu ◽  
Zijian Wang ◽  
...  
Keyword(s):  
Micronucleus Test ◽  
A549 Cells ◽  
Quantitative Structure Activity Relationship ◽  
In Vitro Cytotoxicity ◽  
Cytotoxic Effects ◽  
Qsar Analysis ◽  
Cell Assays ◽  
Micronucleus Tests ◽  
Damaging Effects

Background: More than 2,000 chemicals have been used in the tannery industry. Although some tannery chemicals have been reported to have harmful effects on both human health and the environment, only a few have been subjected to genotoxicity and cytotoxicity evaluations. Objective: This study focused on cytotoxicity and genotoxicity of ten tannery chemicals widely used in China. Materials and Methods: DNA-damaging effects were measured using the SOS/umu test with Salmonella typhimurium TA1535/pSK1002. Chromosome-damaging and cytotoxic effects were determined with the high-content in vitro Micronucleus test (MN test) using the human-derived cell lines MGC-803 and A549. Conclusion: The cytotoxicity of the ten tannery chemicals differed somewhat between the two cell assays, with A549 cells being more sensitive than MGC-803 cells. None of the chemicals induced DNA damage before metabolism, but one was found to have DNA-damaging effects on metabolism. Four of the chemicals, DY64, SB1, DB71 and RR120, were found to have chromosome-damaging effects. A Quantitative Structure-Activity Relationship (QSAR) analysis indicated that one structural feature favouring chemical genotoxicity, Hacceptor-path3-Hacceptor, may contribute to the chromosome-damaging effects of the four MN-test-positive chemicals.

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