Acceleration of wound healing by ultrasound activation of TiO2 in Escherichia coli -infected wounds in mice

2016 ◽  
Vol 105 (8) ◽  
pp. 2344-2351 ◽  
Author(s):  
Koji Osumi ◽  
Sachiko Matsuda ◽  
Naoki Fujimura ◽  
Kentaro Matsubara ◽  
Minoru Kitago ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Wound Healing ◽  
Infected Wounds ◽  
Ultrasound Activation

Preparation of biocompatible wound dressings with dual release of antibiotic and platelet-rich plasma for enhancing infected wound healing

2021 ◽  
pp. 088532822199601
Author(s):  
Linying Shi ◽  
Fang Lin ◽  
Mou Zhou ◽  
Yanhui Li ◽  
Wendan Li ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Wound Dressings ◽  
Inflammatory Factors ◽  
Gelatin Microspheres ◽  
Infected Wounds ◽  
Dual Release

The ever-growing threats of bacterial infection and chronic wound healing have provoked an urgent need for novel antibacterial wound dressings. In this study, we developed a wound dressing for the treatment of infected wounds, which can reduce the inflammatory period (through the use of gentamycin sulfate (GS)) and enhance the granulation stage (through the addition of platelet-rich plasma (PRP)). Herein, the sustained antimicrobial CMC/GMs@GS/PRP wound dressings were developed by using gelatin microspheres (GMs) loading GS and PRP, covalent bonding to carboxymethyl chitosan (CMC). The prepared dressings exhibited high water uptake capability, appropriate porosity, excellent mechanical properties, sustain release of PRP and GS. Meanwhile, the wound dressing showed good biocompatibility and excellent antibacterial ability against Gram-negative and Gram-positive bacteria. Moreover, in vivo experiments further demonstrated that the prepared dressings could accelerate the healing process of E. coli and S. aureus-infected full-thickness wounds i n vivo, reepithelialization, collagen deposition and angiogenesis. In addition, the treatment of CMC/GMs@GS/PRP wound dressing could reduce bacterial count, inhibit pro-inflammatory factors (TNF-α, IL-1β and IL-6), and enhance anti-inflammatory factors (TGF-β1). The findings of this study suggested that biocompatible wound dressings with dual release of GS and PRP have great potential in the treatment of chronic and infected wounds.

scholarly journals The Wound Healing and Antibacterial Activity of Five Ethnomedical Calophyllum inophyllum Oils: An Alternative Therapeutic Strategy to Treat Infected Wounds

PLoS ONE ◽  
2015 ◽  
Vol 10 (9) ◽  
pp. e0138602 ◽  
Author(s):  
Teddy Léguillier ◽  
Marylin Lecsö-Bornet ◽  
Christelle Lémus ◽  
Delphine Rousseau-Ralliard ◽  
Nicolas Lebouvier ◽  
...  
Keyword(s):  
Wound Healing ◽  
Antibacterial Activity ◽  
Therapeutic Strategy ◽  
Calophyllum Inophyllum ◽  
Infected Wounds

A renewed look at silver dressings for wound infections: Ag Oxysalts technology

2021 ◽  
Vol 26 (Sup9) ◽  
pp. S26-S36
Author(s):  
Luxmi Dhoonmoon ◽  
Hayley Turner-Dobbin ◽  
Karen Staines
Keyword(s):  
Wound Healing ◽  
Wound Infection ◽  
Case Studies ◽  
Healing Process ◽  
Systemic Toxicity ◽  
Wound Healing Process ◽  
Wound Infections ◽  
Infected Wounds ◽  
Long Time

Wound infection is an important complicating factor in the wound healing process, and infections can be even more complex and difficult to manage in the case of wounds with biofilms. Silver has been used to treat infected wounds for a long time now, and the strength of the product depends on the number of Ag ions, where the greater the number of ions, the higher and faster the reactivity is. Ag Oxysalts technology—used in 3M Kerracontact Ag dressing—has three times more ions than standard silver dressings. The technology also does not show the typical disadvantages of silver, such as cytotoxicity and systemic toxicity. This article discusses the use of Ag Oxysalts technology for infected wounds and presents case studies to support the efficacy of this product in promoting wound healing.

Evaluation of effectiveness of a new treatment method for healing infected wounds: an animal model

2021 ◽  
Vol 30 (4) ◽  
pp. 312-322
Author(s):  
Alexandr A Basov ◽  
Sergey R Fedosov ◽  
Vadim V Malyshko ◽  
Anna A Elkina ◽  
Oxana M Lyasota ◽  
...  
Keyword(s):  
Wound Healing ◽  
Animal Model ◽  
Active Agent ◽  
Treatment Method ◽  
Healing Time ◽  
Male Adult ◽  
New Device ◽  
Infected Wounds ◽  
New Treatment ◽  
Wound Healing Time

Objective: To evaluate the effectiveness of a new treatment method in healing superficial infected wounds compared with surgical debridement with chlorhexidine solution. Method: In this animal model, two wounds were created on the back of 10 male adult rabbits. Wounds treated by Method 1 were debrided using 0.02% chlorhexidine aqueous solution and an antibiotic topical ointment. Wounds treated by Method 2 wounds were treated using a newly developed device which enabled visual monitoring of the wound as it was treated with various pharmacological solutions (including antiseptic, antiseptic oxidant and an osmotically active agent) specifically formulated for each wound healing stage. Wound area size (using digital planimetry) and time taken to clean the wound were recorded, and biopsies were taken, at the beginning of the study and at various timepoints throughout. Result: It was observed that both wound cleaning and wound healing were accelerated by treatment with method 2 compared with method 1 (by 43.8% and 36.7%, respectively). There were also a significantly smaller number of complications in these wounds [p=0.0044] due to the positive ratios of neutrophils and fibroblasts in the wound cavities (from the third to the fourteenth day after wound modelling). Conclusion: Wounds treated with the new device in method 2 had a shorter wound healing time than wounds treated with a traditional method. The automated influx–outflow of solutions removed any fragments of necrotic tissue from the wound surface. Wounds were able to be monitored without the need to remove dressings. The transparent, airtight film, which allowed for wound monitoring without the need to remove dressings, meant that suturing was not required. This resulted in no complications in the wounds treated by this new method.

A moisturizing chitosan-silk fibroin dressing with silver nanoparticles-adsorbed exosomes for repairing infected wounds

2020 ◽  
Vol 8 (32) ◽  
pp. 7197-7212 ◽  
Author(s):  
Zhiyong Qian ◽  
Yating Bai ◽  
Jin Zhou ◽  
Linhao Li ◽  
Jing Na ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Silver Nanoparticles ◽  
Silk Fibroin ◽  
Broad Spectrum ◽  
Electrolyte Balance ◽  
Infected Wounds ◽  
Multifunctional Properties

The CTS-SF/SA/Ag-Exo dressing possessed multifunctional properties including broad-spectrum antimicrobial activity, promoting wound healing, retaining moisture and maintaining electrolyte balance.

scholarly journals Activity and Effectiveness of Recombinant hEGF Excreted by Escherichia coli BL21 on Wound Healing in Induced Diabetic Mice

2020 ◽  
Vol Volume 12 ◽  
pp. 339-348
Author(s):  
Sriwidodo Sriwidodo ◽  
Iman Permana Maksum ◽  
Toto Subroto ◽  
Nasrul Wathoni ◽  
Anas Subarnas ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Wound Healing ◽  
Diabetic Mice

scholarly journals Regeneration-Competent and -Incompetent Murids Differ in Neutrophil Quantity and Function

2019 ◽  
Vol 59 (5) ◽  
pp. 1138-1149 ◽  
Author(s):  
Jennifer L Cyr ◽  
Thomas R Gawriluk ◽  
John M Kimani ◽  
Balázs Rada ◽  
Wendy T Watford ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Reactive Oxygen Species ◽  
Bone Marrow ◽  
Wound Healing ◽  
Whole Blood ◽  
Oxygen Species ◽  
Pathogen Defense ◽  
Bacteria Killing ◽  
And Function ◽  
Phagocytosis Activity

Abstract Regeneration is rare in mammals, but spiny mice (Acomys spp.) naturally regenerate skin and ear holes. Inflammation is thought to inhibit regeneration during wound healing, but aspects of inflammation contribute to both regeneration and pathogen defense. We compared neutrophil traits among uninjured, regeneration-competent (Acomys: A. cahirinus, A. kempi, A. percivali) and -incompetent (Mus musculus: Swiss Webster, wild-caught strains) murids to test for constitutive differences in neutrophil quantity and function between these groups. Neutrophil quantity differed significantly among species. In blood, Acomys had lower percentages of circulating neutrophils than Mus; and in bone marrow, Acomys had higher percentages of band neutrophils and lower percentages of segmented neutrophils. Functionally, Acomys and Mus neutrophils did not differ in their ability to migrate or produce reactive oxygen species, but Acomys neutrophils phagocytosed more fungal zymosan. Despite this enhanced phagocytosis activity, Acomys neutrophils were not more effective than Mus neutrophils at killing Escherichia coli. Interestingly, whole blood bacteria killing was dominated by serum in Acomys versus neutrophils only or neutrophils and serum in Mus, suggesting that Acomys primarily rely on serum to kill bacteria whereas Mus do not. These subtle differences in neutrophil traits may allow regeneration-competent species to offset damaging effects of inflammation without compromising pathogen defense.

scholarly journals Eradication of Mature Bacterial Biofilms with Concurrent Improvement in Chronic Wound Healing Using Silver Nanoparticle Hydrogel Treatment

Biomedicines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1182
Author(s):  
Hanif Haidari ◽  
Richard Bright ◽  
Sanjay Garg ◽  
Krasimir Vasilev ◽  
Allison J. Cowin ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Wound Closure ◽  
In Vivo Studies ◽  
Release Mechanism ◽  
Impaired Wound Healing ◽  
Biofilm Model ◽  
Infected Wounds ◽  
Delivery Platform

Biofilm-associated infections are a major cause of impaired wound healing. Despite the broad spectrum of anti-bacterial benefits provided by silver nanoparticles (AgNPs), these materials still cause controversy due to cytotoxicity and a lack of efficacy against mature biofilms. Herein, highly potent ultrasmall AgNPs were combined with a biocompatible hydrogel with integrated synergistic functionalities to facilitate elimination of clinically relevant mature biofilms in-vivo combined with improved wound healing capacity. The delivery platform showed a superior release mechanism, reflected by high biocompatibility, hemocompatibility, and extended antibacterial efficacy. In vivo studies using the S. aureus wound biofilm model showed that the AgNP hydrogel (200 µg/g) was highly effective in eliminating biofilm infection and promoting wound repair compared to the controls, including silver sulfadiazine (Ag SD). Treatment of infected wounds with the AgNP hydrogel resulted in faster wound closure (46% closure compared to 20% for Ag SD) and accelerated wound re-epithelization (60% for AgNP), as well as improved early collagen deposition. The AgNP hydrogel did not show any toxicity to tissue and/or organs. These findings suggest that the developed AgNP hydrogel has the potential to be a safe wound treatment capable of eliminating infection and providing a safe yet effective strategy for the treatment of infected wounds.

scholarly journals Composites Based on Gellan Gum, Alginate and Nisin-Enriched Lipid Nanoparticles for the Treatment of Infected Wounds

2021 ◽  
Vol 23 (1) ◽  
pp. 321
Author(s):  
Katarzyna Reczyńska-Kolman ◽  
Kinga Hartman ◽  
Konrad Kwiecień ◽  
Monika Brzychczy-Włoch ◽  
Elżbieta Pamuła
Keyword(s):  
Wound Healing ◽  
Bacterial Infections ◽  
Average Particle Size ◽  
Lipid Nanoparticles ◽  
Gellan Gum ◽  
Average Particle ◽  
Hydrogel Matrix ◽  
Swelling Capacity ◽  
Infected Wounds ◽  
Methods Of Treatment

Due to growing antimicrobial resistance to antibiotics, novel methods of treatment of infected wounds are being searched for. The aim of this research was to develop a composite wound dressing based on natural polysaccharides, i.e., gellan gum (GG) and a mixture of GG and alginate (GG/Alg), containing lipid nanoparticles loaded with antibacterial peptide—nisin (NSN). NSN-loaded stearic acid-based nanoparticles (NP_NSN) were spherical with an average particle size of around 300 nm and were cytocompatible with L929 fibroblasts for up to 500 µg/mL. GG and GG/Alg sponges containing either free NSN (GG + NSN and GG/Alg + NSN) or NP_NSN (GG + NP_NSN and GG/Alg + NP_NSN) were highly porous with a high swelling capacity (swelling ratio above 2000%). Encapsulation of NSN within lipid nanoparticles significantly slowed down NSN release from GG-based samples for up to 24 h (as compared to GG + NSN). The most effective antimicrobial activity against Gram-positive Streptococcus pyogenes was observed for GG + NP_NSN, while in GG/Alg it was decreased by interactions between NSN and Alg, leading to NSN retention within the hydrogel matrix. All materials, except GG/Alg + NP_NSN, were cytocompatible with L929 fibroblasts and did not cause an observable delay in wound healing. We believe that the developed materials are promising for wound healing application and the treatment of bacterial infections in wounds.

Sign in / Sign up

Export Citation Format

Share Document