Real-time, noninvasive optical coherence tomography of cross-sectional living cell-sheetsin vitroandin vivo

2014 ◽  
Vol 103 (6) ◽  
pp. 1267-1273 ◽  
Author(s):  
Mari Kobayashi ◽  
Yuji Haraguchi ◽  
Tatsuya Shimizu ◽  
Kiminori Mizuuchi ◽  
Hiroshi Iseki
Keyword(s):  
Optical Coherence Tomography ◽  
Real Time ◽  
Living Cell ◽  
Optical Coherence ◽  
Cross Sectional

scholarly journals Modern Trends in Imaging V: Optical Coherence Tomography for Rapid Tissue Screening and Directed Histological Sectioning

2012 ◽  
Vol 35 (3) ◽  
pp. 129-143 ◽  
Author(s):  
Woonggyu Jung ◽  
Stephen A. Boppart
Keyword(s):  
Optical Coherence Tomography ◽  
Real Time ◽  
Image Guidance ◽  
Diagnostic Yield ◽  
Imaging Modality ◽  
Imaging Techniques ◽  
Optical Coherence ◽  
Sampling Errors ◽  
Cross Sectional

In pathology, histological examination of the “gold standard” to diagnose various diseases. It has contributed significantly toward identifying the abnormalities in tissues and cells, but has inherent drawbacks when used for fast and accurate diagnosis. These limitations include the lack ofin vivoobservation in real time and sampling errors due to limited number and area coverage of tissue sections. Its diagnostic yield also varies depending on the ability of the physician and the effectiveness of any image guidance technique that may be used for tissue screening during excisional biopsy. In order to overcome these current limitations of histology-based diagnostics, there are significant needs for either complementary or alternative imaging techniques which perform non-destructive, high resolution, and rapid tissue screening. Optical coherence tomography (OCT) is an emerging imaging modality which allows real-time cross-sectional imaging with high resolutions that approach those of histology. OCT could be a very promising technique which has the potential to be used as an adjunct to histological tissue observation when it is not practical to take specimens for histological processing, when large areas of tissue need investigating, or when rapid microscopic imaging is needed. This review will describe the use of OCT as an image guidance tool for fast tissue screening and directed histological tissue sectioning in pathology.

scholarly journals Measurement of Oral Epithelial Thickness by Optical Coherence Tomography

Diagnostics ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 90 ◽  
Author(s):  
Stasio ◽  
Lauritano ◽  
Iquebal ◽  
Romano ◽  
Gentile ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Oral Cavity ◽  
Real Time ◽  
Optical Coherence ◽  
Cross Sectional ◽  
Tissue Microstructure ◽  
Imaging Device ◽  
Epithelial Thickness ◽  
Healthy Mucosa

Optical coherence tomography (OCT) is a real-time, in-situ, non-invasive imaging device that is able to perform a cross-sectional evaluation of tissue microstructure based on the specific intensity of back-scattered and reflected light. The aim of the present study was to define normal values of epithelial thickness within the oral cavity. OCT measurements of epithelial thickness were performed in 28 healthy patients at six different locations within the oral cavity. Image analysis was performed using Image J 1.52 software. The healthy epithelium has a mean thickness of 335.59 ± 150.73 µm. According to its location within the oral cavity, the epithelium showed highest values in the region of the buccal mucosa (659.79 µm) and the thinnest one was observed in the mouth’s floor (100.07 µm). OCT has been shown to be useful for the evaluation of oral mucosa in vivo and in real time. Our study provides reference values for the epithelial thickness of multiple sites within the oral cavity. Knowledge of the thickness values of healthy mucosa is, therefore, of fundamental importance.

Doppler Optical Coherence Tomography: Real-Time Optical Sectioning for Microfluidics

2009 ◽  
Author(s):  
Yeh-Chan Ahn ◽  
Matthew Brenner ◽  
Zhongping Chen
Keyword(s):  
Optical Coherence Tomography ◽  
Real Time ◽  
Doppler Ultrasound ◽  
Acoustic Waves ◽  
Motion Artifacts ◽  
Fourier Domain ◽  
Optical Coherence ◽  
Cross Sectional ◽  
Measurable Velocity ◽  
Doppler Optical Coherence Tomography

Doppler optical coherence tomography (DOCT) is an emerging imaging modality demonstrated in 1991 for the first time and is a functional extension of optical coherence tomography (OCT) to including flow measurement. DOCT allows not only high-resolution, non-invasive, cross-sectional imaging but also simultaneous real-time visualization of sample structure and flow. DOCT is often compared to clinical Doppler ultrasound. However, the spatial resolution of clinical Doppler ultrasound is limited to approximately 100 μm due to the relatively long wavelength of acoustic waves. DOCT takes advantage of the short coherence length of broadband light sources in order to achieve cross-sectional images with micrometer (2–10 μm) scale resolution. DOCT is also superior to ultrasound in that DOCT is operated in non-contact-mode. The last four years have witnessed an era of technology revolution in DOCT, introduced by the Fourier-domain technology that shows tremendous advantage over time-domain DOCT. Fourier-domain Doppler optical coherence tomography (FDDOCT) instruments have higher imaging speed and higher system sensitivity which are able to overcome motion artifacts and enhance minimum measurable velocity, respectively. Because of the aforementioned merits, FDDOCT has a broad range of clinical applications including ophthalmology, cardiology, urology, etc with information of tissue microstructure and blood flow. However, FDDOCT has seldom been applied to diagnose microfluidic devices. In this keynote paper, system configuration, principle behind, and applications of FDDOCT for microfluidics will be covered.

Nondestructive evaluation of fused filament fabrication 3D printed structures using optical coherence tomography

2020 ◽  
Vol 26 (10) ◽  
pp. 1853-1860
Author(s):  
Lucas Ramos De Pretto ◽  
Marcello Magri Amaral ◽  
Anderson Zanardi de Freitas ◽  
Marcus Paulo Raele
Keyword(s):  
Optical Coherence Tomography ◽  
Nondestructive Evaluation ◽  
Real Time ◽  
Tomographic Reconstruction ◽  
Optical Coherence ◽  
Evaluation Tool ◽  
Fused Filament Fabrication ◽  
Cross Sectional ◽  
Content Type ◽  
3D Printed

Purpose The quality of components under fused filament fabrication (FFF) is related to the correct filament spacing and bonding of successively deposited layers and is evaluated mainly by scanning electron microscopy (SEM). However, it is a destructive technique and real-time evaluation is not possible. Optical coherence tomography (OCT), on the other hand, is an optical method that acquires cross-sectional images non-invasively and in real-time. Therefore, this paper aims to propose and validate the use of OCT as a non-destructive quality evaluation tool for FFF using Polylactic Acid (PLA) filaments. Design/methodology/approach PLA three-dimensional (3D) printed samples were made in a variety of nozzle temperatures and mesh spacing. These samples were fractured in liquid nitrogen and inspected using SEM (as a gold standard) to evaluate dimensions and morphology, then the samples were evaluated by OCT in the same area, allowing the results confrontation. Findings Our results indicate a good correlation between OCT and SEM for the dimensional assessment of layers. When the filament was extruded in lower temperatures, the OCT images presented sharply defined interfaces between layers, in contrary to higher nozzle temperatures, denoting better fusion between them. However, higher extruding temperatures are incurred in greater deviations from nominal dimensions of the mesh. Finally, we demonstrate the advantage of a full 3D tomographic reconstruction to inspect within a FFF sample, which enabled the inspection of “hidden” information, not visible on a single cross-sectional cut. Originality/value This paper proposes OCT as a novel and nondestructive evaluation tool for FFF.

scholarly journals Comparison of swept-source versus spectral-domain optical coherence tomography angiography for detection of macular neovascularization

2021 ◽  
Author(s):  
Anna Lentzsch ◽  
Laura Schöllhorn ◽  
Christel Schnorr ◽  
Robert Siggel ◽  
Sandra Liakopoulos
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence Tomography Angiography ◽  
Spectral Domain ◽  
Optical Coherence ◽  
Cross Sectional ◽  
Swept Source ◽  
En Face ◽  
Increased Sensitivity ◽  
Sensitivity Specificity ◽  
Subretinal Hyperreflective Material

Abstract Purpose To compare swept-source (SS) versus spectral-domain (SD) optical coherence tomography angiography (OCTA) for the detection of macular neovascularization (MNV). Methods In this prospective cohort study, 72 eyes of 54 patients with subretinal hyperreflective material (SHRM) and/or pigment epithelial detachment (PED) on OCT possibly corresponding to MNV in at least one eye were included. OCTA scans were acquired using two devices, the PLEX Elite 9000 SS-OCTA and the Spectralis SD-OCTA. Fluorescein angiography (FA) was used as reference. Two graders independently evaluated en face OCTA images using a preset slab as well as a manually modified slab, followed by a combination of en face and cross-sectional OCTA. Results Sensitivity (specificity) for the automated slabs was 51.7% (93.0%) for SS-OCTA versus 58.6% (95.3%) for SD-OCTA. Manual modification of segmentation increased sensitivity to 79.3% for SS-OCTA but not for SD-OCTA (58.6%). The combination of en face OCTA with cross-sectional OCTA reached highest sensitivity values (SS-OCTA: 82.8%, SD-OCTA: 86.2%), and lowest number of cases with discrepancies between SS-OCTA and SD-OCTA (4.2%). Fleiss kappa as measure of concordance between FA, SS-OCTA, and SD-OCTA was 0.56 for the automated slabs, 0.60 for the manual slabs, and 0.73 (good agreement) for the combination of en face OCTA with cross-sectional OCTA. Concordance to FA was moderate for the automated slabs and good for manual slabs and combination with cross-sectional OCTA of both devices. Conclusion Both devices reached comparable results regarding the detection of MNV on OCTA. Sensitivity for MNV detection and agreement between devices was best when evaluating a combination of en face and cross-sectional OCTA.

scholarly journals Coherence tomography with broad bandwidth extreme ultraviolet and soft X-ray radiation

2021 ◽  
Vol 127 (4) ◽  
Author(s):  
S. Skruszewicz ◽  
S. Fuchs ◽  
J. J. Abel ◽  
J. Nathanael ◽  
J. Reinhard ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Near Infrared ◽  
Extreme Ultraviolet ◽  
Infrared Light ◽  
Imaging Method ◽  
Optical Coherence ◽  
Axial Resolution ◽  
Cross Sectional ◽  
X Ray ◽  
Broad Bandwidth

AbstractWe present an overview of recent results on optical coherence tomography with the use of extreme ultraviolet and soft X-ray radiation (XCT). XCT is a cross-sectional imaging method that has emerged as a derivative of optical coherence tomography (OCT). In contrast to OCT, which typically uses near-infrared light, XCT utilizes broad bandwidth extreme ultraviolet (XUV) and soft X-ray (SXR) radiation (Fuchs et al in Sci Rep 6:20658, 2016). As in OCT, XCT’s axial resolution only scales with the coherence length of the light source. Thus, an axial resolution down to the nanometer range can be achieved. This is an improvement of up to three orders of magnitude in comparison to OCT. XCT measures the reflected spectrum in a common-path interferometric setup to retrieve the axial structure of nanometer-sized samples. The technique has been demonstrated with broad bandwidth XUV/SXR radiation from synchrotron facilities and recently with compact laboratory-based laser-driven sources. Axial resolutions down to 2.2 nm have been achieved experimentally. XCT has potential applications in three-dimensional imaging of silicon-based semiconductors, lithography masks, and layered structures like XUV mirrors and solar cells.

scholarly journals Utilization potential of intraluminal optical coherence tomography for the Eustachian tube

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hayoung Byun ◽  
Yeon Hoon Kim ◽  
Jingchao Xing ◽  
Su-Jin Shin ◽  
Seung Hwan Lee ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Eustachian Tube ◽  
Balloon Dilatation ◽  
Optical Coherence ◽  
Cross Sectional ◽  
Anatomical Structures ◽  
Complex Anatomy ◽  
Before And After ◽  
Tubal Lumen ◽  
Oct Image

AbstractImaging the Eustachian tube is challenging because of its complex anatomy and limited accessibility. This study fabricated a fiber-based optical coherence tomography (OCT) catheter and investigated its potential for assessing the Eustachian tube anatomy. A customized OCT system and an imaging catheter, termed the Eustachian OCT, were developed for visualizing the Eustachian tube. Three male swine cadaver heads were used to study OCT image acquisition and for subsequent histologic correlation. The imaging catheter was introduced through the nasopharyngeal opening and reached toward the middle ear. The OCT images were acquired from the superior to the nasopharyngeal opening before and after Eustachian tube balloon dilatation. The histological anatomy of the Eustachian tube was compared with corresponding OCT images, The new, Eustachian OCT catheter was successfully inserted in the tubal lumen without damage. Cross-sectional images of the tube were successfully obtained, and the margins of the anatomical structures including cartilage, mucosa lining, and fat could be successfully delineated. After balloon dilatation, the expansion of the cross-sectional area could be identified from the OCT images. Using the OCT technique to assess the Eustachian tube anatomy was shown to be feasible, and the fabricated OCT image catheter was determined to be suitable for Eustachian tube assessment.

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