Anisotropic temperature sensitive chitosan-based injectable hydrogels mimicking cartilage matrix

2014 ◽  
Vol 103 (6) ◽  
pp. 1149-1160 ◽  
Author(s):  
Kenneth J. Walker ◽  
Sundararajan V. Madihally
Keyword(s):  
Cartilage Matrix ◽  
Temperature Sensitive ◽  
Injectable Hydrogels ◽  
Anisotropic Temperature

Heparin-based temperature-sensitive injectable hydrogels for protein delivery

2015 ◽  
Vol 3 (45) ◽  
pp. 8892-8901 ◽  
Author(s):  
Hye Jin Sim ◽  
Thavasyappan Thambi ◽  
Doo Sung Lee
Keyword(s):  
Protein Delivery ◽  
Temperature Sensitive ◽  
Sustained Delivery ◽  
Injectable Hydrogels

Polysaccharide-based biodegradable, biocompatible and temperature-sensitive injectable hydrogels have been developed for the sustained delivery of proteins.

Precise synchronization of hyperthermia–chemotherapy: photothermally induced on-demand release from injectable hydrogels of gold nanocages

Nanoscale ◽  
2018 ◽  
Vol 10 (42) ◽  
pp. 20020-20032 ◽  
Author(s):  
Jiangshan Wan ◽  
Shinan Geng ◽  
Hao Zhao ◽  
Xiaole Peng ◽  
Jiabao Xu ◽  
...  
Keyword(s):  
Temperature Sensitive ◽  
Injectable Hydrogels ◽  
On Demand ◽  
Gold Nanocages

Temperature sensitive polymers were modified onto gold nanocages, and the resultant temperature sensitive hydrogels achieved precise synchronization of hyperthermia and chemotherapy by the on-demand release.

scholarly journals Engineering of Optimized Hydrogel Formulations for Cartilage Repair

Polymers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1526
Author(s):  
Yao Fu ◽  
Bram Zoetebier ◽  
Sanne Both ◽  
Pieter J. Dijkstra ◽  
Marcel Karperien
Keyword(s):  
Mechanical Properties ◽  
Cartilage Regeneration ◽  
Cartilage Matrix ◽  
Cartilage Tissue ◽  
Injectable Hydrogels ◽  
Cellular Behavior ◽  
Injectable Scaffolds ◽  
Hybrid Hydrogels ◽  
Promote Cell Survival ◽  
The Ideal

The ideal scaffold for cartilage regeneration is expected to provide adequate mechanical strength, controlled degradability, adhesion, and integration with the surrounding native tissue. As it does this, it mimics natural ECMs functions, which allow for nutrient diffusion and promote cell survival and differentiation. Injectable hydrogels based on tyramine (TA)-functionalized hyaluronic acid (HA) and dextran (Dex) are a promising approach for cartilage regeneration. The properties of the hydrogels used in this study were adjusted by varying polymer concentrations and ratios. To investigate the changes in properties and their effects on cellular behavior and cartilage matrix formation, different ratios of HA- and dextran-based hybrid hydrogels at both 5 and 10% w/v were prepared using a designed mold to control generation. The results indicated that the incorporation of chondrocytes in the hydrogels decreased their mechanical properties. However, rheological and compression analysis indicated that 5% w/v hydrogels laden with cells exhibit a significant increase in mechanical properties after 21 days when the constructs are cultured in a chondrogenic differentiation medium. Moreover, compared to the 10% w/v hydrogels, the 5% w/v hybrid hydrogels increased the deposition of the cartilage matrix, especially in constructs with a higher Dex–TA content. These results indicated that 5% w/v hybrid hydrogels with 25% HA–TA and 75% Dex–TA have a high potential as injectable scaffolds for cartilage tissue regeneration.

scholarly journals Thermosensitive alginate–gelatin–nitrogen-doped carbon dots scaffolds as potential injectable hydrogels for cartilage tissue engineering applications

RSC Advances ◽  
2021 ◽  
Vol 11 (30) ◽  
pp. 18423-18431
Author(s):  
Mojgan Ghanbari ◽  
Masoud Salavati-Niasari ◽  
Fatemeh Mohandes
Keyword(s):  
Tissue Engineering ◽  
Gelation Time ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Temperature Sensitive ◽  
Gelation Temperature ◽  
Injectable Hydrogels ◽  
Nitrogen Doped ◽  
Doped Carbon Dots ◽  
Nitrogen Doped Carbon

The low gelation time (120 s) and gelation temperature at body temperature (37 °C) make oxidized alginate/gelatin/NCDs hydrogels suitable as temperature-sensitive injectable hydrogels for cartilage tissue engineering.

Oncogenesis and the Lucké Virus

1968 ◽  
Vol 26 ◽  
pp. 200-201
Author(s):  
A. E. Vatter ◽  
J. Zambernard
Keyword(s):  
Rna Viruses ◽  
Vegetative State ◽  
Temperature Sensitive ◽  
Structural Level ◽  
Oncogenic Viruses ◽  
Dna Viruses ◽  
Leopard Frogs ◽  
Renal Adenocarcinoma ◽  
Induced Tumors ◽  
Northern Leopard Frogs

Oncogenic viruses, like viruses in general, can be divided into two classes, those that contain deoxyribonucleic acid (DNA) and those that contain ribonucleic acid (RNA). The RNA viruses have been recovered readily from the tumors which they cause whereas, the DNA-virus induced tumors have not yielded the virus. Since DNA viruses cannot be recovered, the bulk of present day investigations have been concerned with RNA viruses.The Lucké renal adenocarcinoma is a spontaneous tumor which occurs in northern leopard frogs (Rana pipiens) and has received increased attention in recent years because of its probable viral etiology. This hypothesis was first advanced by Lucké after he observed intranuclear inclusions in some of the tumor cells. Tumors with inclusions were examined at the fine structural level by Fawcett who showed that they contained immature and mature virus˗like particles.The use of this system in the study of oncogenic tumors offers several unique features, the virus has been shown to contain DNA and it can be recovered from the tumor, also, it is temperature sensitive. This latter feature is of importance because the virus can be transformed from a latent to a vegetative state by lowering or elevating the environmental temperature.

Effects of EDTA, Hyaluronidase and Collagenase on Epiphyseal Cartilage Matrix

1968 ◽  
Vol 26 ◽  
pp. 56-57
Author(s):  
H. Clarke Anderson ◽  
Priscilla R. Coulter
Keyword(s):  
Light And Electron Microscopy ◽  
Matrix Vesicles ◽  
Cartilage Matrix ◽  
Collagen Fibrils ◽  
Epiphyseal Cartilage ◽  
Dense Matrix ◽  
Type Iv ◽  
Bovine Testicular Hyaluronidase ◽  
The Matrix ◽  
Testicular Hyaluronidase

Epiphyseal cartilage matrix contains fibrils and particles of at least 5 different types: 1. Banded collagen fibrils, present throughout the matrix, but not seen in the lacunae. 2. Non-periodic fine fibrils <100Å in diameter (Fig. 1), which are most notable in the lacunae, and may represent immature collagen. 3. Electron dense matrix granules (Fig. 1) which are often attached to fine fibrils and collagen fibrils, and probably contain protein-polysaccharide although the possibility of a mineral content has not been excluded. 4. Matrix vesicles (Fig. 2) which show a selective distribution throughout the epiphysis, and may play a role in calcification. 5. Needle-like apatite crystals (Fig. 2).Blocks of formalin-fixed epiphysis from weanling mice were digested with the following agents in 0.1M phosphate buffer: a) 5% ethylenediaminetetraacetate (EDTA) at pH 8.3, b) 0.015% bovine testicular hyaluronidase (Sigma, type IV, 750 units/mg) at pH 5.5, and c) 0.1% collagenase (Worthington, chromatograhically pure, 200 units/mg) at pH 7.4. All digestions were carried out at 37°C overnight. Following digestion tissues were examined by light and electron microscopy to determine changes in the various fibrils and particles of the matrix.

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