Molecular and tissue responses in the healing of rat calvarial defects after local application of simvastatin combined with alpha tricalcium phosphate

2009 ◽  
Vol 9999B ◽  
pp. NA-NA ◽  
Author(s):  
Myat Nyan ◽  
Takayuki Miyahara ◽  
Kanako Noritake ◽  
Jia Hao ◽  
Reena Rodriguez ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Local Application ◽  
Tissue Responses ◽  
Calvarial Defects

scholarly journals Bone Fracture-Treatment Method: Fixing 3D-Printed Polycaprolactone Scaffolds with Hydrogel Type Bone-Derived Extracellular Matrix and β-Tricalcium Phosphate as an Osteogenic Promoter

2021 ◽  
Vol 22 (16) ◽  
pp. 9084
Author(s):  
Seokhwan Yun ◽  
Dami Choi ◽  
Dong-Jin Choi ◽  
Songwan Jin ◽  
Won-Soo Yun ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Bone Formation ◽  
Tricalcium Phosphate ◽  
Bone Fractures ◽  
Critical Diameter ◽  
Micro Computed Tomography ◽  
Reconstruction Methods ◽  
Osteoblast Cell Line ◽  
Calvarial Defects ◽  
3D Printed

Bone formation and growth are crucial for treating bone fractures. Improving bone-reconstruction methods using autologous bone and synthetic implants can reduce the recovery time. Here, we investigated three treatments using two different materials, a bone-derived decellularized extracellular matrix (bdECM) and β-tricalcium phosphate (β-TCP), individually and in combination, as osteogenic promoter between bone and 3D-printed polycaprolactone scaffold (6-mm diameter) in rat calvarial defects (8-mm critical diameter). The materials were tested with a human pre-osteoblast cell line (MG63) to determine the effects of the osteogenic promoter on bone formation in vitro. A polycaprolactone (PCL) scaffold with a porous structure was placed at the center of the in vivo rat calvarial defects. The gap between the defective bone and PCL scaffold was filled with each material. Animals were sacrificed four weeks post-implantation, and skull samples were preserved for analysis. The preserved samples were scanned by micro-computed tomography and analyzed histologically to examine the clinical benefits of the materials. The bdECM–β-TCP mixture showed faster bone formation and a lower inflammatory response in the rats. Therefore, our results imply that a bdECM–β-TCP mixture is an ideal osteogenic promoter for treating fractures.

scholarly journals 3D-Printed Barrier Membrane Using Mixture of Polycaprolactone and Beta-Tricalcium Phosphate for Regeneration of Rabbit Calvarial Defects

Materials ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3280
Author(s):  
Jun-Young Lee ◽  
Jin-Young Park ◽  
In-Pyo Hong ◽  
Su-Hee Jeon ◽  
Jae-Kook Cha ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Tissue Reaction ◽  
Synthetic Bone ◽  
Tissue Integration ◽  
Osteogenic Cells ◽  
Barrier Membrane ◽  
Beta Tricalcium Phosphate ◽  
Calvarial Defects ◽  
3D Printed ◽  
Good Biocompatibility

Background: Polycarprolactone and beta tricalcium phosphate (PCL/β-TCP) are resorbable biomaterials that exhibit ideal mechanical properties as well as high affinity for osteogenic cells. Aim: Objective of this study was to evaluate healing and tissue reaction to the PCL/β-TCP barrier membrane in the rabbit calvaria model for guided bone regeneration. Materials and Methods: The PCL/β-TCP membranes were 3D printed. Three circular defects were created in calvaria of 10 rabbits. The three groups were randomly allocated for each specimen: (i) sham control; (ii) PCL/β-TCP membrane (PCL group); and (iii) PCL/β-TCP membrane with synthetic bone graft (PCL-BG group). The animals were euthanized after two (n = 5) and eight weeks (n = 5) for volumetric and histomorphometric analyses. Results: The greatest augmented volume was achieved by the PCL-BG group at both two and eight weeks (p < 0.01). There was a significant increase in new bone after eight weeks in the PCL group (p = 0.04). The PCL/β-TCP membrane remained intact after eight weeks with slight degradation, and showed good tissue integration. Conclusions: PCL/β-TCP membrane exhibited good biocompatibility, slow degradation, and ability to maintain space over eight weeks. The 3D-printed PCL/β-TCP membrane is a promising biomaterial that could be utilized for reconstruction of critical sized defects.

Cranioplasty With Adipose-Derived Stem Cells and Biomaterial: A Novel Method for Cranial Reconstruction

Neurosurgery ◽  
2011 ◽  
Vol 68 (6) ◽  
pp. 1535-1540 ◽  
Author(s):  
Tuomo Thesleff ◽  
Kai Lehtimäki ◽  
Tero Niskakangas ◽  
Bettina Mannerström ◽  
Susanna Miettinen ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Stem Cells ◽  
Tricalcium Phosphate ◽  
Adipose Derived Stem Cells ◽  
Hounsfield Units ◽  
Beta Tricalcium Phosphate ◽  
Alloplastic Materials ◽  
Satisfactory Outcome ◽  
Calvarial Defects ◽  
Autologous Bone

Abstract BACKGROUND: There is no optimal method for reconstruction of large calvarial defects. Because of the limitations of autologous bone grafts and alloplastic materials, new methods for performing cranioplasties are needed. OBJECTIVE: To create autologous bone to repair cranial defects. METHODS: We performed a cranioplasty procedure with this new method in 4 patients who had large calvarial defects of different etiologies. We used autologous adipose-derived stem cells seeded in beta-tricalcium phosphate granules. For 2 patients, we used a bilaminate technique with resorbable mesh. RESULTS: During follow-up, there were no clinically relevant postoperative complications. The computed tomography scans revealed satisfactory outcome in ossification, and in the clinical examinations, the outcomes were good. The cranioplasty was measured in Hounsfield units from each computed tomography scan. The Hounsfield units increased gradually to equal the value of bone. CONCLUSION: The combination of scaffold material such as beta-tricalcium phosphate and autologous adipose-derived stem cells constitutes a promising model for reconstruction of human large cranial defects. The success of these clinical cases paves way for further studies and clinical applications to turn this method into a reliable treatment regimen.

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