Development of a biomimetic collagen-hydroxyapatite scaffold for bone tissue engineering using a SBF immersion technique

2009 ◽  
Vol 90B (2) ◽  
pp. 584-591 ◽  
Author(s):  
Amir A. Al-Munajjed ◽  
Niamh A. Plunkett ◽  
John P. Gleeson ◽  
Tim Weber ◽  
Christian Jungreuthmayer ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Immersion Technique ◽  
Hydroxyapatite Scaffold

scholarly journals Effects of the Sintering Process on Nacre-Derived Hydroxyapatite Scaffolds for Bone Engineering

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3129
Author(s):  
Rohaya Megat Abdul Wahab ◽  
Nurmimie Abdullah ◽  
Shahrul Hisham Zainal Ariffin ◽  
Che Azurahanim Che Abdullah ◽  
Farinawati Yazid
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Nacreous Layer ◽  
Sintering Process ◽  
Micro Computed Tomography ◽  
Native Bone ◽  
Porous Microstructure ◽  
Hydroxyapatite Scaffold ◽  
Osteoblast Markers

A hydroxyapatite scaffold is a suitable biomaterial for bone tissue engineering due to its chemical component which mimics native bone. Electronic states which present on the surface of hydroxyapatite have the potential to be used to promote the adsorption or transduction of biomolecules such as protein or DNA. This study aimed to compare the morphology and bioactivity of sinter and nonsinter marine-based hydroxyapatite scaffolds. Field emission scanning electron microscopy (FESEM) and micro-computed tomography (microCT) were used to characterize the morphology of both scaffolds. Scaffolds were co-cultured with 5 × 104/cm2 of MC3T3-E1 preosteoblast cells for 7, 14, and 21 days. FESEM was used to observe the cell morphology, and MTT and alkaline phosphatase (ALP) assays were conducted to determine the cell viability and differentiation capacity of cells on both scaffolds. Real-time polymerase chain reaction (rtPCR) was used to identify the expression of osteoblast markers. The sinter scaffold had a porous microstructure with the presence of interconnected pores as compared with the nonsinter scaffold. This sinter scaffold also significantly supported viability and differentiation of the MC3T3-E1 preosteoblast cells (p < 0.05). The marked expression of Col1α1 and osteocalcin (OCN) osteoblast markers were also observed after 14 days of incubation (p < 0.05). The sinter scaffold supported attachment, viability, and differentiation of preosteoblast cells. Hence, sinter hydroxyapatite scaffold from nacreous layer is a promising biomaterial for bone tissue engineering.

The efficacy of polycaprolactone/hydroxyapatite scaffold in combination with mesenchymal stem cells for bone tissue engineering

2015 ◽  
Vol 104 (1) ◽  
pp. 264-271 ◽  
Author(s):  
Boontharika Chuenjitkuntaworn ◽  
Thanaphum Osathanon ◽  
Nunthawan Nowwarote ◽  
Pitt Supaphol ◽  
Prasit Pavasant
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Hydroxyapatite Scaffold

Fish scale derived hydroxyapatite scaffold for bone tissue engineering

2016 ◽  
Vol 121 ◽  
pp. 112-124 ◽  
Author(s):  
B Mondal ◽  
S. Mondal ◽  
A. Mondal ◽  
N. Mandal
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Fish Scale ◽  
Hydroxyapatite Scaffold

Characterization of Phosphate Glass/Hydroxyapatite Scaffold for Palate Repair

2014 ◽  
Vol 931-932 ◽  
pp. 301-305 ◽  
Author(s):  
Wassanai Wattanutchariya ◽  
Pornpatima Yenbut
Keyword(s):  
Tissue Engineering ◽  
Compressive Strength ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Phosphate Glass ◽  
Replication Method ◽  
Scaffold Structure ◽  
Palate Repair ◽  
Hydroxyapatite Scaffold

Bone grafting is the standard treatment for cleft palate patients. However, a downside to this method is that it requires multiple surgeries to fill the gap in the mouth. Bone tissue engineering can be employed as a solution to this problem to fabricate artificial bone based on synthetic biomaterials. The objectives of this study focus on preparing phosphate glass and hydroxyapatite (HA) as well as developing appropriate forming conditions for scaffold based on the polymeric replication method. Various glass compositions and sintering temperatures were examined in order to investigate scaffold structure, compressive strength, and biodegradability. Amounts of CaO and sintering temperatures were varied in order to explore their impacts on scaffold properties. Results from XRD clearly show that phosphate glass and HA can be successfully synthesized using natural materials. It was also found that polymeric foam replication can be successfully used for scaffold fabrication and the scaffold microstructure revealed that the appropriate pore size for bone tissue engineering is in the 240 360 μm range. Results indicate that biodegradability can be regulated by the amount of CaO used. For example, specimens with the highest level of biodegradability were obtained from 30 mol% of CaO composition. The highest compressive strength (6.54 MPa) was obtained from scaffold containing 40 mol% of CaO, sintered at 750 °C.

A Novel Polycaprolactone/Hydroxyapatite Scaffold for Bone Tissue Engineering

2007 ◽  
pp. 265-268
Author(s):  
Ho Hyun Song ◽  
Mi Kyong Yoo ◽  
Hyun Seuk Moon ◽  
Yun Jaie Choi ◽  
Hyun Chul Lee ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Hydroxyapatite Scaffold

3D gel-printing of hydroxyapatite scaffold for bone tissue engineering

2019 ◽  
Vol 45 (1) ◽  
pp. 1163-1170 ◽  
Author(s):  
Huiping Shao ◽  
Jianzhuang He ◽  
Tao Lin ◽  
Zhinan Zhang ◽  
Yumeng Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Hydroxyapatite Scaffold
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