Simulated body fluid and the novel bioactive materials derived from it

2019 ◽  
Vol 107 (5) ◽  
pp. 968-977 ◽  
Author(s):  
Tadashi Kokubo ◽  
Seiji Yamaguchi
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
The Novel ◽  
Bioactive Materials

Apatite Formation on Zirconia (Y-TZP) Coated with Carbonate Apatite in Simulated Body Fluid

2019 ◽  
Vol 829 ◽  
pp. 145-150
Author(s):  
Astrie Yumeisa ◽  
Lisda Damayanti ◽  
Taufik Sumarsongko ◽  
Andrie Harmaji ◽  
Arief Cahyanto
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
Bone Structure ◽  
Calcium Phosphates ◽  
Bone Defects ◽  
Control Group ◽  
Apatite Formation ◽  
Carbonate Apatite ◽  
Actin Ring ◽  
Bioactive Materials

Abstract. Various bioactive calcium phosphates such as hydroxyapatite (HA) and carbonate apatite (CO3Ap) have been widely studied due to their biocompatibility and osteoconductivity when implanted into bone defects. CO3Ap has the ability to adapt bone structure and induce bone regeneration; so that it can be categorized as resorbable bioactive materials. CO3Ap induced much stronger response such as cell adhesion and actin ring formation to osteoclast-like cells rather than HA. The aim of this study is to evaluate the bioactivity on zirconia (Y-TZP) coated with CO3Ap using simulated body fluid (SBF). Twenty Y-TZP ZrO2 disks with a 12-mm diameter and 1-mm thickness were employed as the samples. The disks were divided into two groups which the control group without CO3Ap coating and tested group with CO3Ap coating. Disks samples are dipped into CO3Ap suspension for one minute and stored in 37°C incubator for 24 hours. The disks were soaked in SBF for 1, 4, and 7 day(s) at 36.5°C. The obtained apatite crystals were characterized by scanning electron microscopy (SEM). It was found that the apatite formation on the tested group was greater than the control group. The EDS pattern showed the presence of Ca and P on the control and tested group after SBF soaking, which indicate the apatite deposition on the disks’ surface. However, the Ca and P on the tested group was higher compared to the control group. The formation of apatite layer on the disks’ surface is bioactivity indicator of CO3Ap.

Preparation of a Bioactive Xenograft

2007 ◽  
Vol 330-332 ◽  
pp. 99-102
Author(s):  
Hyung Sup Kim ◽  
Yong Keun Lee ◽  
Bum Soon Lim ◽  
Chong Pyong Chung ◽  
Sang Hoon Rhee
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
Size Range ◽  
Bovine Bone ◽  
Mixed Solution ◽  
Bioactive Materials ◽  
Carbonated Apatite ◽  
Heat Treated ◽  
New Process ◽  
Low Crystalline

The method which gives a low crystalline hydroxyl carbonated apatite forming ability to originally non-bioactive high crystalline hydroxyapatite surface was newly developed. The granules of trabecular bovine bone, which had a size range from 212 to 1000 μm, were defatted, deproteinized, and then heat-treated at 1000 oC for 3 hours to remove organics completely. They were treated with the mixed solution of calcium chloride and calcium hydroxide in Soxhlet’s apparatus at 100 oC for 3 days and then dried completely. Low crystalline hydroxyl carbonated apatite was observed to occur on the surface of high crystalline hydroxyapatite granules after soaking them into the simulated body fluid (SBF) for 1 week. This method is likely to have a potential to be used as a new process to give a bioactivity to originally non-bioactive materials.

Gap Effect on the Heterogeneous Nucleation of Apatite on Thermally Oxidized Titanium Substrate

2007 ◽  
Vol 361-363 ◽  
pp. 621-624 ◽  
Author(s):  
Atsushi Sugino ◽  
Keita Uetsuki ◽  
Kanji Tsuru ◽  
Satoshi Hayakawa ◽  
Chikara Ohtsuki ◽  
...  
Keyword(s):  
Simulated Body Fluid ◽  
Thermal Oxidation ◽  
Body Fluid ◽  
Mouth Opening ◽  
Titanium Substrate ◽  
Gap Effect ◽  
Apatite Formation ◽  
The Novel ◽  
Essential Condition ◽  
Novel Technique

Apatite formation on the surface of materials in body environment is an essential condition to show osteoconduction after implantation in bony defects. This study reports the novel technique for providing the apatite-forming ability to titanium metals by only controlling the spatial gap and thermal oxidation. Two pieces of titanium thermally oxidized at 400 °C were set together like V-letter with varied mouth opening. They showed the formation of apatite on both facing surface after exposure to a simulated body fluid (SBF) proposed by Kokubo and his colleagues, when the gap was less than approximately 600 μm. Moreover, specimens with micro-grooves of 500 μm in depth and 200-1000 μm in width was able to form apatite in SBF with in 7 days, after they were thermally oxidized at 400 oC. These results indicated that the titanium metals were provided with apatite-forming ability, i.e. osteoconduction, due to controlled gap with thermally oxidized surface. Hence, we conclude that bioactive titanium substrate showing osteoconduction can be produced by designed machining followed by thermal oxidation at an appropriate temperature.

Biocompatability of Precipitation-Enhancing Anodic Oxidation on Titanium as Implant Material

2007 ◽  
Vol 330-332 ◽  
pp. 593-596
Author(s):  
Keun Taek Oh ◽  
Kyoung Nam Kim ◽  
Dong Hoo Han ◽  
M.K. Chung ◽  
S.H. Hwang
Keyword(s):  
Cell Proliferation ◽  
Simulated Body Fluid ◽  
Physical Properties ◽  
Surface Topography ◽  
Anodic Oxidation ◽  
Body Fluid ◽  
The Novel ◽  
Machined Surface ◽  
Mtt Test ◽  
Fluid Cell

The composition of the electrolytes used in the process of anodic oxidation was modified to generate the novel anodic oxidation. The surface topography of the novel anodic oxidation showed that the pore/crater structures with the precipitants attached over the surface were observed in SEM. The physical properties and biocompatibility of the surface with the novel anodic oxidation were compared with that of anodic oxidation and machined surface of titanium. XRD, Bioactivity in a simulated body fluid, cell proliferation, and MTT test were performed to evaluate the differences among different surfaces.

Fabrication of Novel Hemostatic Film with Oxidized Cellulose and Sugar-Containing Hydroxyapatite

2018 ◽  
Vol 782 ◽  
pp. 84-90 ◽  
Author(s):  
Yeon Jeong Noh ◽  
Tomohiro Umeda ◽  
Yoshiro Musha ◽  
Kiyoshi Itatani
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
Cellulose Nanofibers ◽  
Bone Diseases ◽  
Oxidized Cellulose ◽  
The Novel ◽  
Transparent Films

The novel hemostatic film for the surgery of bone diseases was fabricated using TEMPO(2,2,6,6-tetramethylpiperidine-1-oxyl)-oxidized cellulose nanofibers (TOCNs), and phosphoryl oligosaccharides of calcium (POs-Ca) or sugar-containing hydroxyapatite (s-Ca10(PO4)6(OH)2; s-HAp). Three kinds of the hydrophilic and transparent films with the thicknesses of 10 to 20 μm were fabricated, i.e., TOCN, POs-Ca-added TOCN and s-HAp-added TOCN films. Among these films, the uptake amount of the simulated body fluid by s-HAp-added TOCN film was as high as 5,543%, which was expected to quickly stop bleeding of larger amount of blood for the hemostasis.

Formation of Hydroxyapatite on Titanium Oxides in Simulated Body Fluid under UV Irradiation

2010 ◽  
Vol 654-656 ◽  
pp. 2257-2260 ◽  
Author(s):  
Masato Ueda ◽  
Hiroki Sai ◽  
Masahiko Ikeda ◽  
Michiharu Ogawa
Keyword(s):  
Simulated Body Fluid ◽  
Uv Irradiation ◽  
Body Fluid ◽  
Uv Light ◽  
Titanium Oxides ◽  
Bioactive Materials ◽  
Hno3 Solution ◽  
Gel Layer ◽  
Tio2 Gel ◽  
Perovskite Type

Hydroxyapatite (HAp), Ca10(PO4)6(OH)2, is known to precipitate on bioactive materials by soaking in simulated body fluid (SBF). The formation of HAp on TiO2 and CaTiO3 surfaces under continuous ultraviolet (UV) irradiation was investigated in this study. Pure Ti substrates were chemically treated with H2O2/ HNO3 solution at 353 K for 20 min to form a TiO2 gel layer. The samples were then hydrothermally treated with an aqueous NH3 or an aqueous Ca(OH)2 solution in an autoclave at 453 K for 12 h. An adhesive and sufficiently crystallized anatase-type TiO2 film or perovskite-type CaTiO3 film could be synthesized on the Ti surface, respectively. The samples were immersed in SBF in darkness or under UV irradiation. The UV irradiation promoted the formation of HAp, which may be due to the generation of functional Ti-OH or Ti-O groups on both surfaces. On the other hand, the UV light also produces electron-hole pairs in the product films. In TiO2, much photogenerated holes migrated to the surface and repelled the Ca2+ ions in the solution. As a consequence, the UV irradiation suppressed the formation of HAp thin film on the surface of TiO2.

A Study on the Bioactivity of Nano-Titania/Hydroxyapatite Composite

2006 ◽  
Vol 309-311 ◽  
pp. 411-414 ◽  
Author(s):  
Bang Cheng Yang ◽  
Qi Feng Yu ◽  
Ji Yong Chen ◽  
Xing Dong Zhang
Keyword(s):  
Heat Treatment ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Apatite Formation ◽  
Bioactive Materials ◽  
Hydroxyapatite Composite

The bioactivity of a composite of titania and hydroxyapatite was studied in vitro in this paper. After the titania ceramics was added 10% HA, it could induce apatite formation in simulated body fluid in 2d, while the pure titania ceramics could not induced apatite formation even after 14d. After the composite of titania and HA was subjected to alkali-heat treatment, it has a faster speed for apatite formation in SBF than the composite without treatment. When the osteoblast was cultured on the materials, the amount of osteoblasts attaching on the composite was more than that on the pure titania ceramics. It has the most osteoblasts cells on the composite subjected to alkali-heat treatment. These results showed that the composite of titania and HA is a bioactive materials, while the alkali-heat treatment could improved the bioactivity of this composite.

IN-VITRO BEHAVIOUR OF BIPHASIC CALCIUM PHOSPHATE WITH DIFFERENT RATIO OF SILICA CONTENT IN SIMULATED BODY FLUID

2015 ◽  
Vol 23 (1) ◽  
pp. 1-14
Author(s):  
Sudirman Sahid ◽  
◽  
Nor Shahida Kader Bashah ◽  
Salina Sabudin ◽  
◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Biphasic Calcium Phosphate ◽  
Silica Content

Phase evolution of hydroxapatite coatings suspension plasma sprayed using variable parameters in simulated body fluid

2010 ◽  
Vol 204 (8) ◽  
pp. 1236-1246 ◽  
Author(s):  
Romain d'Haese ◽  
Lech Pawlowski ◽  
Muriel Bigan ◽  
Roman Jaworski ◽  
Marc Martel
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
Phase Evolution ◽  
Variable Parameters ◽  
Plasma Sprayed
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