Selection of the optimum 3D-printed pore and the surface modification techniques for tissue engineering tracheal scaffold in vivo reconstruction

2018 ◽  
Vol 107 (2) ◽  
pp. 360-370 ◽  
Author(s):  
Shu Pan ◽  
Yi Zhong ◽  
Yibo Shan ◽  
Xueying Liu ◽  
Yuanfan Xiao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Surface Modification ◽  
3D Printed ◽  
Surface Modification Techniques ◽  
Selection Of

scholarly journals Fabrication of 3D-Printed Interpenetrating Hydrogel Scaffolds for Promoting Chondrogenic Differentiation

Polymers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 2146
Author(s):  
Jian Guan ◽  
Fu-zhen Yuan ◽  
Zi-mu Mao ◽  
Hai-lin Zhu ◽  
Lin Lin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Elastic Modulus ◽  
Chondrogenic Differentiation ◽  
Marker Genes ◽  
Self Healing ◽  
Polyethylene Glycol Diacrylate ◽  
3D Microenvironment ◽  
3D Printed

The limited self-healing ability of cartilage necessitates the application of alternative tissue engineering strategies for repairing the damaged tissue and restoring its normal function. Compared to conventional tissue engineering strategies, three-dimensional (3D) printing offers a greater potential for developing tissue-engineered scaffolds. Herein, we prepared a novel photocrosslinked printable cartilage ink comprising of polyethylene glycol diacrylate (PEGDA), gelatin methacryloyl (GelMA), and chondroitin sulfate methacrylate (CSMA). The PEGDA-GelMA-CSMA scaffolds possessed favorable compressive elastic modulus and degradation rate. In vitro experiments showed good adhesion, proliferation, and F-actin and chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) on the scaffolds. When the CSMA concentration was increased, the compressive elastic modulus, GAG production, and expression of F-actin and cartilage-specific genes (COL2, ACAN, SOX9, PRG4) were significantly improved while the osteogenic marker genes of COL1 and ALP were decreased. The findings of the study indicate that the 3D-printed PEGDA-GelMA-CSMA scaffolds possessed not only adequate mechanical strength but also maintained a suitable 3D microenvironment for differentiation, proliferation, and extracellular matrix production of BMSCs, which suggested this customizable 3D-printed PEGDA-GelMA-CSMA scaffold may have great potential for cartilage repair and regeneration in vivo.

Surface modification of polyurethane towards promoting the ex vivo cytocompatibility and in vivo biocompatibility for hypopharyngeal tissue engineering

2012 ◽  
Vol 28 (4) ◽  
pp. 607-616 ◽  
Author(s):  
Zhisen Shen ◽  
Cheng Kang ◽  
Jingjing Chen ◽  
Dong Ye ◽  
Shijie Qiu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Surface Modification ◽  
Ex Vivo

Near-Infrared Emitting Dual-Stimuli-Responsive Carbon Dots from Endogenous Bile Pigments

Nanoscale ◽  
2021 ◽  
Author(s):  
Parinaz Fathi ◽  
Parikshit Moitra ◽  
Madeleine M. McDonald ◽  
Mandy Brigitte Esch ◽  
Dipanjan Pan
Keyword(s):  
Surface Modification ◽  
Carbon Dots ◽  
Biomedical Applications ◽  
Near Infrared ◽  
Bile Pigments ◽  
Careful Selection ◽  
Stimuli Responsive ◽  
Carbon Dot ◽  
Surface Modification Techniques ◽  
Selection Of

Carbon dots are biocompatible nanoparticles suitable for a variety of biomedical applications. Careful selection of carbon dot precursors and surface modification techniques has allowed for the development of carbon dots...

Articulating Biomaterials

2015 ◽  
pp. 218-267 ◽  
Author(s):  
Vamsi Krishna Balla ◽  
Mitun Das ◽  
Someswar Datta ◽  
Biswanath Kundu
Keyword(s):  
Surface Modification ◽  
Clinical Performance ◽  
Decisive Role ◽  
Surface Characteristics ◽  
Surface Modification Techniques ◽  
Biological Environment ◽  
Surface Modified ◽  
In Vivo Effects

This chapter examines the importance of surface characteristics such as microstructure, composition, crystallographic texture, and surface free energy in achieving desired biocompatibility and tribological properties thereby improving in vivo life of artificial articulating implants. Current implants often fail prematurely due to inadequate mechanical, tribological, biocompatibility, and osseointegration properties, apart from issues related to design and surgical procedures. For long-term in vivo stability, artificial implants intended for articulating joint replacement must exhibit long-term stable articulation surface without stimulating undesirable in vivo effects. Since the implant's surface plays a vital and decisive role in their response to biological environment, and vice versa, surface modification of implants assumes a significant importance. Therefore, overview on important surface modification techniques, their capabilities, properties of modified surfaces/implants are presented in the chapter. The clinical performance of surface modified implants and new surfaces for potential next-generation articulating implant applications are discussed at the end.

Titanium Surface Modification Techniques for Implant Fabrication – From Microscale to the Nanoscale

2010 ◽  
Vol 5 ◽  
pp. 39-56 ◽  
Author(s):  
Karthikeyan Subramani
Keyword(s):  
Surface Modification ◽  
Dental Implants ◽  
Tio2 Nanotubes ◽  
Titanium Surface ◽  
In Vivo Studies ◽  
Contact Ratio ◽  
Implant Surface ◽  
Surface Modification Techniques

This manuscript reviews about titanium surface modification techniques for its application in orthopaedic and dental implants. There are a few limitations in the long term prognosis of orthopaedic and dental implants. Poor osseointegration with bone, periimplant infection leading to implant failure and short term longevity demanding revision surgery, are to mention a few. Micro- and nanoscale modification of titanium surface using physicochemical, morphological and biochemical approaches have resulted in higher bone to implant contact ratio and improved osseointegration. With recent advances in micro, nano-fabrication techniques and multidisciplinary research studies focusing on bridging biomaterials for medical applications, TiO2 nanotubes have been extensively studied for implant applications. The need for titanium implant surface that can closely mimic the nanoscale architecture of human bone has become a priority. For such purpose, TiO2 nanotubes of different dimensions and architectural fashions at the nanoscale level are being evaluated. This manuscript discusses in brief about the in-vitro and in-vivo studies on titanium surface modification techniques. This manuscript also addresses the recent studies done on such nanotubular surfaces for the effective delivery of osteoinductive growth factors and anti bacterial/ anti inflammatory drugs to promote osseointegration and prevent peri-implant infection.

scholarly journals Surface modification of small intestine submucosa in tissue engineering

2020 ◽  
Vol 7 (4) ◽  
pp. 339-348 ◽  
Author(s):  
Pan Zhao ◽  
Xiang Li ◽  
Qin Fang ◽  
Fanglin Wang ◽  
Qiang Ao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Surface Modification ◽  
Small Intestine ◽  
Cell Adhesion ◽  
Great Influence ◽  
Small Intestine Submucosa ◽  
Proliferation And Differentiation ◽  
Tissue Reconstruction

Abstract With the development of tissue engineering, the required biomaterials need to have the ability to promote cell adhesion and proliferation in vitro and in vivo. Especially, surface modification of the scaffold material has a great influence on biocompatibility and functionality of materials. The small intestine submucosa (SIS) is an extracellular matrix isolated from the submucosal layer of porcine jejunum, which has good tissue mechanical properties and regenerative activity, and is suitable for cell adhesion, proliferation and differentiation. In recent years, SIS is widely used in different areas of tissue reconstruction, such as blood vessels, bone, cartilage, bladder and ureter, etc. This paper discusses the main methods for surface modification of SIS to improve and optimize the performance of SIS bioscaffolds, including functional group bonding, protein adsorption, mineral coating, topography and formatting modification and drug combination. In addition, the reasonable combination of these methods also offers great improvement on SIS surface modification. This article makes a shallow review of the surface modification of SIS and its application in tissue engineering.

scholarly journals Parathyroid hormone (1-34) promotes the effects of 3D printed scaffold-seeded bone marrow mesenchymal stem cells on meniscus regeneration

2020 ◽  
Author(s):  
Wen Zhao ◽  
Tong Zou ◽  
Hao Cui ◽  
Yangou Lv ◽  
Dengke Gao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Culture Time ◽  
Marrow Mesenchymal Stem Cells ◽  
3D Printed ◽  
Scaffold Group ◽  
And Cartilage

Abstract Background Cell-based tissue engineering represent a promising management for meniscus repair and regeneration. The present study aimed to investigate whether the injection of parathyroid hormone (PTH) (1–34) could promote the regeneration and chondroprotection of 3D printed scaffold seeded with bone marrow mesenchymal stem cells (BMSCs) in a canine total meniscal meniscectomy model. Methods 3D printed poly(e-caprolactone) scaffold seeded with BMSCs was cultured in vitro, and the effects of in vitro culture time on cell growth and matrix synthesis of the BMSCs-scaffold construct were evaluated by microscopic observation and cartilage matrix content detection at 7, 14, 21, and 28 days. After that, the tissue-engineered meniscus based on BMSCs–scaffold cultured for the appropriate culture time was selected for in vivo implantation. Sixteen dogs were randomly divided into four groups: PTH + BMSCs–scaffold, BMSCs–scaffold, total meniscectomy, and sham operation. The regeneration of the implanted tissue and the degeneration of articular cartilage were assessed by gross, histological, and immunohistochemical analysis at 12 weeks postoperatively. Results In vitro study showed that the glycosaminoglycan (GAG)/DNA ratio and the expression of collagen type II (Col2) were significantly higher on day 21 as compared to the other time points. In vivo study showed that, compared with the BMSCs–scaffold group, the PTH + BMSCs–scaffold group showed better regeneration of the implanted tissue and greater similarity to native meniscus with respect to gross appearance, cells composition, and cartilage extracellular matrix deposition. This group also showed less expression of terminal differentiation markers of BMSC chondrogenesis as well as lower cartilage degeneration with less damage on the knee cartilage surface, higher expression of Col2 and lower expression of degeneration markers. Conclusions Our results demonstrated that PTH (1–34) promotes the regenerative and chondroprotective effects of the BMSCs–3D printed meniscal scaffold in a canine model, and thus their combination could be a promising strategy for meniscus tissue engineering.

A review on 3D printing in tissue engineering applications

2022 ◽  
Vol 0 (0) ◽  
Author(s):  
Mohan Prasath Mani ◽  
Madeeha Sadia ◽  
Saravana Kumar Jaganathan ◽  
Ahmad Zahran Khudzari ◽  
Eko Supriyanto ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
3D Printing ◽  
Cell Adherence ◽  
Engineering Applications ◽  
Proliferation And Differentiation ◽  
3D Printed ◽  
Printing Techniques

Abstract In tissue engineering, 3D printing is an important tool that uses biocompatible materials, cells, and supporting components to fabricate complex 3D printed constructs. This review focuses on the cytocompatibility characteristics of 3D printed constructs, made from different synthetic and natural materials. From the overview of this article, inkjet and extrusion-based 3D printing are widely used methods for fabricating 3D printed scaffolds for tissue engineering. This review highlights that scaffold prepared by both inkjet and extrusion-based 3D printing techniques showed significant impact on cell adherence, proliferation, and differentiation as evidenced by in vitro and in vivo studies. 3D printed constructs with growth factors (FGF-2, TGF-β1, or FGF-2/TGF-β1) enhance extracellular matrix (ECM), collagen I content, and high glycosaminoglycan (GAG) content for cell growth and bone formation. Similarly, the utilization of 3D printing in other tissue engineering applications cannot be belittled. In conclusion, it would be interesting to combine different 3D printing techniques to fabricate future 3D printed constructs for several tissue engineering applications.

scholarly journals Fabrication and Application of a 3D-Printed Poly-ε-Caprolactone Cage Scaffold for Bone Tissue Engineering

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Siyi Wang ◽  
Rong Li ◽  
Yongxiang Xu ◽  
Dandan Xia ◽  
Yuan Zhu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Synthetic Material ◽  
Hybrid Scaffold ◽  
Natural Bone Mineral ◽  
Application Potential ◽  
3D Printed

Poly-ε-caprolactone (PCL) is a promising synthetic material in bone tissue engineering (BTE). Particularly, the introduction of rapid prototyping (RP) represents the possibility of manufacturing PCL scaffolds with customized appearances and structures. Bio-Oss is a natural bone mineral matrix with significant osteogenic effects; however, it has limitations in being constructed and maintained into specific shapes and sites. In this study, we used RP and fabricated a hollow-structured cage-shaped PCL scaffold loaded with Bio-Oss to form a hybrid scaffold for BTE. Moreover, we adopted NaOH surface treatment to improve PCL hydrophilicity and enhance cell adhesion. The results showed that the NaOH-treated hybrid scaffold could enhance the osteogenesis of human bone marrow-derived mesenchymal stem cells (hBMMSCs) both in vitro and in vivo. Altogether, we reveal a novel hybrid scaffold that not only possesses osteoinductive function to promote bone formation but can also be fabricated into specific forms. This scaffold design may have great application potential in bone tissue engineering.

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