Development of novel three-dimensional scaffolds based on bacterial nanocellulose for tissue engineering and regenerative medicine: Effect of processing methods, pore size, and surface area

2018 ◽  
Vol 107 (2) ◽  
pp. 348-359 ◽  
Author(s):  
Marlon Osorio ◽  
Patricia Fernández-Morales ◽  
Piedad Gañán ◽  
Robín Zuluaga ◽  
Herbert Kerguelen ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Pore Size ◽  
Surface Area ◽  
Three Dimensional ◽  
Bacterial Nanocellulose ◽  
Processing Methods

scholarly journals Additive Manufacturing of Biopolymers for Tissue Engineering and Regenerative Medicine: An Overview, Potential Applications, Advancements, and Trends

2021 ◽  
Vol 2021 ◽  
pp. 1-20 ◽  
Author(s):  
Dhinakaran Veeman ◽  
M. Swapna Sai ◽  
P. Sureshkumar ◽  
T. Jagadeesha ◽  
L. Natrayan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Additive Manufacturing ◽  
3D Printing ◽  
Regenerative Medicine ◽  
Tissue Regeneration ◽  
Three Dimensional ◽  
Porous Scaffolds ◽  
Wide Range ◽  
Potential Applications ◽  
Pharmaceutical Industries

As a technique of producing fabric engineering scaffolds, three-dimensional (3D) printing has tremendous possibilities. 3D printing applications are restricted to a wide range of biomaterials in the field of regenerative medicine and tissue engineering. Due to their biocompatibility, bioactiveness, and biodegradability, biopolymers such as collagen, alginate, silk fibroin, chitosan, alginate, cellulose, and starch are used in a variety of fields, including the food, biomedical, regeneration, agriculture, packaging, and pharmaceutical industries. The benefits of producing 3D-printed scaffolds are many, including the capacity to produce complicated geometries, porosity, and multicell coculture and to take growth factors into account. In particular, the additional production of biopolymers offers new options to produce 3D structures and materials with specialised patterns and properties. In the realm of tissue engineering and regenerative medicine (TERM), important progress has been accomplished; now, several state-of-the-art techniques are used to produce porous scaffolds for organ or tissue regeneration to be suited for tissue technology. Natural biopolymeric materials are often better suited for designing and manufacturing healing equipment than temporary implants and tissue regeneration materials owing to its appropriate properties and biocompatibility. The review focuses on the additive manufacturing of biopolymers with significant changes, advancements, trends, and developments in regenerative medicine and tissue engineering with potential applications.

Development of Scaffold Building Units and Assembly for Tissue Engineering Using Fused Deposition Modelling

2009 ◽  
Vol 83-86 ◽  
pp. 269-274 ◽  
Author(s):  
Syed H. Masood ◽  
Kadhim Alamara
Keyword(s):  
Tissue Engineering ◽  
Pore Size ◽  
Fused Deposition Modeling ◽  
Porous Scaffold ◽  
Cell Structure ◽  
Three Dimensional ◽  
Three Dimensions ◽  
Fused Deposition Modelling ◽  
Fused Deposition ◽  
Unit Cells

In tissue engineering (TE), a porous scaffold structure of biodegradable material is required as a template to guide the proliferation, growth and development of cells appropriately in three dimensions. The scaffold must meet design requirements of appropriate porosity, pore size and interconnected structure to allow cell proliferation and adhesion. This paper presents a methodology for design and manufacture of TE scaffolds with varying porosity by employing open structure building units and Fused Deposition Modeling (FDM) rapid prototyping technique. A computer modeling approach for constructing and assembly of three-dimensional unit cell structure is presented to provide a solution of scaffolds design that can potentially meet the diverse requirements of TE applications. A parametric set of open polyhedral unit cells is used to assist the user in designing the required micro-architecture of the scaffold with required porosity and pore size and then the Boolean operation is used to create the scaffold of a given CAD model from the designed microstructure. The procedure is verified by fabrication of physical scaffolds using the commercial FDM system.

Design and additive manufacturing of porous titanium scaffolds for optimum cell viability in bone tissue engineering

2020 ◽  
pp. 095440542093756
Author(s):  
Bingbing Li ◽  
Bani Davod Hesar ◽  
Yiwen Zhao ◽  
Li Ding
Keyword(s):  
Tissue Engineering ◽  
Cell Viability ◽  
Bone Tissue Engineering ◽  
Pore Size ◽  
Bone Tissue ◽  
Structural Strength ◽  
Three Dimensional ◽  
Porous Titanium ◽  
Nih3t3 Cells ◽  
Pore Sizes

Pore size, external shape, and internal complexity of additively manufactured porous titanium scaffolds are three primary determinants of cell viability and structural strength of scaffolds in bone tissue engineering. To obtain an optimal design with the combination of all three determinants, four scaffolds each with a unique topology (external geometry and internal structure) were designed and varied the pore sizes of each scaffold 3 times. For each topology, scaffolds with pore sizes of 300, 400, and 500 µm were designed. All designed scaffolds were additively manufactured in material Ti6Al4V by the direct metal laser melting machine. Compression test was conducted on the scaffolds to assure meeting minimum compressive strength of human bone. The effects of pore size and topology on the cell viability of the scaffolds were analyzed. The 12 scaffolds were ultrasonically cleaned and seeded with NIH3T3 cells. Each scaffold was seeded with 1 million cells. After 32 days of culturing, the cells were fixed for their three-dimensional architecture preservation and to obtain scanning electron microscope images.

scholarly journals Hydrogel Properties and Their Impact on Regenerative Medicine and Tissue Engineering

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 5795
Author(s):  
Adam Chyzy ◽  
Marta E. Plonska-Brzezinska
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Physicochemical Properties ◽  
Three Dimensional ◽  
Modern Medicine ◽  
Wound Treatment ◽  
Chemical Modifications

Hydrogels (HGs), as three-dimensional structures, are widely used in modern medicine, including regenerative medicine. The use of HGs in wound treatment and tissue engineering is a rapidly developing sector of medicine. The unique properties of HGs allow researchers to easily modify them to maximize their potential. Herein, we describe the physicochemical properties of HGs, which determine their subsequent applications in regenerative medicine and tissue engineering. Examples of chemical modifications of HGs and their applications are described based on the latest scientific reports.

Preparation and Characterization of Ha/Gel/β-TCP Microspheres Composite Porous Scaffold

2018 ◽  
Vol 782 ◽  
pp. 103-115
Author(s):  
Yang Zi Zhao ◽  
You Fa Wang
Keyword(s):  
Tissue Engineering ◽  
Compressive Strength ◽  
Hyaluronic Acid ◽  
Pore Size ◽  
Porous Scaffold ◽  
Three Dimensional ◽  
Swelling Ratio ◽  
Cell Compatibility ◽  
Hydrogel Scaffolds

Being one of the three elements of tissue engineering, three-dimensional porous structure scaffold plays an important role in tissue engineering. As it not only prvovide cells for the life, but also serves as a template to guide tissue regeneration and control of organizational structure and other functions. In this study, hyaluronic acid and gelatin are successfully cross-linked by 1-ethyl- (3-dimethylaminopropyl) -carbodiimide hydrochloride (EDC) , and compound β-TCP microspheres to prepare porous hydrogel scaffolds. The microspheres were analyzed by X-ray diffraction (XRD). The scaffolds were characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). At the same time, the compressive strength, swelling ratio, degradation of the scaffold were tested. To assess the in vitro cell compatibility of the scaffolds, mouse L929 fibroblasts were seeded onto scaffolds for cell morphology and cell viability studies. The results showed that the pore size of the porous scaffold can be adjusted by changing the ratio of gelatin to hyaluronic acid (HA), increasing the proportion of hyaluronic acid in a certain range, pore size will be significantly increased. With the increase of the proportion of hyaluronic acid in the scaffold, the swelling ratio and the degradation rate also increased. The compressive strength of the scaffold increased with the increase of the proportion of gelatin. The appropriate ratio of β-TCP can promote cell growth and proliferation.

scholarly journals Regenerative Therapies Using Cell Sheet-Based Tissue Engineering for Cardiac Disease

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Yuji Haraguchi ◽  
Tatsuya Shimizu ◽  
Masayuki Yamato ◽  
Teruo Okano
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Cardiac Disease ◽  
Heart Function ◽  
Cell Sheet ◽  
Three Dimensional ◽  
Tissue Cell ◽  
Myocardial Tissue ◽  
Therapeutic Effects ◽  
Cardiac Diseases

At present, cardiac diseases are a major cause of morbidity and mortality in the world. Recently, a cell-based regenerative medicine has appeared as one of the most potential and promising therapies for improving cardiac diseases. As a new generational cell-based regenerative therapy, tissue engineering is focused. Our laboratory has originally developed cell sheet-based scaffold-free tissue engineering. Three-dimensional myocardial tissue fabricated by stacking cardiomyocyte sheets, which are tightly interconnected to each other through gap junctions, beats simultaneously and macroscopically and shows the characteristic structures of native heart tissue. Cell sheet-based therapy cures the damaged heart function of animal models and is clinically applied. Cell sheet-based tissue engineering has a promising and enormous potential in myocardial tissue regenerative medicine and will cure many patients suffering from severe cardiac disease. This paper summarizes cell sheet-based tissue engineering and its satisfactory therapeutic effects on cardiac disease.

Engineered Biomimetic Nanofibers for Regenerative Medicine

2010 ◽  
Vol 76 ◽  
pp. 114-124
Author(s):  
Seeram Ramakrishna ◽  
Jayarama Reddy Venugopal ◽  
Susan Liao
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Regenerative Medicine ◽  
Cardiac Tissue ◽  
Three Dimensional ◽  
Electrospun Nanofibers ◽  
Cost Effective ◽  
Bioactive Molecules ◽  
Natural Polymers ◽  
Hematopoietic Stem

Attempts have been made to fabricate nanofibrous scaffolds to mimic the chemical composition and structural properties of extracellular matrix (ECM) for tissue/organ regeneration. Nanofibers with various patterns have been successfully produced from synthetic and natural polymers through a relatively simple technique of electrospinning. The resulting patterns can mimic some of the diverse tissue-specific orientation and three-dimensional (3D) fibrous structure. Studies on cell-nanofiber interactions have revealed the importance of nanotopography on cell adhesion, proliferation and differentiation. Our recent data showed that hematopoietic stem cells (HSCs) as well as mesenchymal stem cells (MSCs) can rapidly and effectively attached to the functionalized nanofibers. Mineralized 3D nanofibrous scaffold with bone marrow derived MSCs has been applied for bone tissue engineering. The use of injectable nanofibers for cardiac tissue engineering applications is attractive as they allow for the encapsulation of cardiomyocytes/MSCs as well as bioactive molecules for the repair of myocardial infarction. Duplicate 3D heart helix microstructure by the nanofibrous cardiac patch might provide functional support for infarcted myocardium. Furthermore, clinical applications of electrospun nanofibers for regenerative medicine are highly feasible due to the ease and flexibility of fabrication with the cost-effective method of making nanofibers.

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