Hydroxyapatite nanowires modified polylactic acid membrane plays barrier/osteoinduction dual roles and promotes bone regeneration in a rat mandible defect model

2018 ◽  
Vol 106 (12) ◽  
pp. 3099-3110 ◽  
Author(s):  
Jing Han ◽  
Baojin Ma ◽  
Hongrui Liu ◽  
Ting Wang ◽  
Fang Wang ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Polylactic Acid ◽  
Defect Model ◽  
Dual Roles ◽  
Mandible Defect ◽  
Rat Mandible

scholarly journals Three-dimensional printed polylactic acid scaffold integrated with BMP-2 laden hydrogel for precise bone regeneration

2021 ◽  
Vol 25 (1) ◽  
Author(s):  
Misun Cha ◽  
Yuan-Zhe Jin ◽  
Jin Wook Park ◽  
Kyung Mee Lee ◽  
Shi Huan Han ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Polylactic Acid ◽  
Three Dimensional ◽  
Burst Release ◽  
Defect Model ◽  
Ectopic Ossification ◽  
Significant Difference ◽  
3D Printed

Abstract Background Critical bone defects remain challenges for clinicians, which cannot heal spontaneously and require medical intervention. Following the development of three-dimensional (3D) printing technology is widely used in bone tissue engineering for its outstanding customizability. The 3D printed scaffolds were usually accompanied with growth factors, such as bone morphometric protein 2 (BMP-2), whose effects have been widely investigated on bone regeneration. We previously fabricated and investigated the effect of a polylactic acid (PLA) cage/Biogel scaffold as a carrier of BMP-2. In this study, we furtherly investigated the effect of another shape of PLA cage/Biogel scaffold as a carrier of BMP-2 in a rat calvaria defect model and an ectopic ossification (EO) model. Method The PLA scaffold was printed with a basic commercial 3D printer, and the PLA scaffold was combined with gelatin and alginate-based Biogel and BMP-2 to induce bone regeneration. The experimental groups were divided into PLA scaffold, PLA scaffold with Biogel, PLA scaffold filled with BMP-2, and PLA scaffold with Biogel and BMP-2 and were tested both in vitro and in vivo. One-way ANOVA with Bonferroni post-hoc analysis was used to determine whether statistically significant difference exists between groups. Result The in vitro results showed the cage/Biogel scaffold released BMP-2 with an initial burst release and followed by a sustained slow-release pattern. The released BMP-2 maintained its osteoinductivity for at least 14 days. The in vivo results showed the cage/Biogel/BMP-2 group had the highest bone regeneration in the rat calvarial defect model and EO model. Especially, the bone regenerated more regularly in the EO model at the implanted sites, which indicated the cage/Biogel had an outstanding ability to control the shape of regenerated bone. Conclusion In conclusion, the 3D printed PLA cage/Biogel scaffold system was proved to be a proper carrier for BMP-2 that induced significant bone regeneration and induced bone formation following the designed shape.

scholarly journals Bacterial cellulose-hydroxyapatite composites with osteogenic growth peptide (OGP) or pentapeptide OGP on bone regeneration in critical-size calvarial defect model

2015 ◽  
Vol 103 (10) ◽  
pp. 3397-3406 ◽  
Author(s):  
Suzane C. Pigossi ◽  
Guilherme J. P. L. de Oliveira ◽  
Livia S. Finoti ◽  
Rafael Nepomuceno ◽  
Luis Carlos Spolidorio ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bacterial Cellulose ◽  
Critical Size ◽  
Calvarial Defect ◽  
Defect Model

scholarly journals Retraction note: Histopathological features of bone regeneration in a canine segmental ulnar defect model

2016 ◽  
Vol 11 (1) ◽  
Author(s):  
Rahim Hobbenaghi ◽  
Pariya Mahboob ◽  
Siamak Saifzadeh ◽  
Javad Javanbakht ◽  
Javad Yaghoobi Yeganeh Manesh ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Defect Model ◽  
Histopathological Features

Effect of CD146 + SHED on bone regeneration in a mouse calvaria defect model

Oral Diseases ◽  
2021 ◽  
Author(s):  
Kodai Rikitake ◽  
Ryo Kunimatsu ◽  
Yuki Yoshimi ◽  
Kengo Nakajima ◽  
Tomoka Hiraki ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Defect Model ◽  
Mouse Calvaria

scholarly journals Additive manufacturing of PLA-based scaffolds intended for bone regeneration and strategies to improve their biological properties

e-Polymers ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 571-599
Author(s):  
Ricardo Donate ◽  
Mario Monzón ◽  
María Elena Alemán-Domínguez
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Additive Manufacturing ◽  
Bone Regeneration ◽  
Polylactic Acid ◽  
State Of The Art ◽  
Biological Properties ◽  
Design Stage ◽  
Bone Scaffolds ◽  
Regeneration Of Bone

AbstractPolylactic acid (PLA) is one of the most commonly used materials in the biomedical sector because of its processability, mechanical properties and biocompatibility. Among the different techniques that are feasible to process this biomaterial, additive manufacturing (AM) has gained attention recently, as it provides the possibility of tuning the design of the structures. This flexibility in the design stage allows the customization of the parts in order to optimize their use in the tissue engineering field. In the recent years, the application of PLA for the manufacture of bone scaffolds has been especially relevant, since numerous studies have proven the potential of this biomaterial for bone regeneration. This review contains a description of the specific requirements in the regeneration of bone and how the state of the art have tried to address them with different strategies to develop PLA-based scaffolds by AM techniques and with improved biofunctionality.

Nanotubes in nanofibers: Antibacterial multilayered polylactic acid/halloysite/gentamicin membranes for bone regeneration application

2018 ◽  
Vol 160 ◽  
pp. 95-105 ◽  
Author(s):  
Malgorzata Karolina Pierchala ◽  
Maziyar Makaremi ◽  
Hui Li Tan ◽  
Janarthanan Pushpamalar ◽  
Saravanan Muniyandy ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Polylactic Acid

scholarly journals Bone Regeneration Capacity of Newly Developed Uncalcined/Unsintered Hydroxyapatite and Poly-l-lactide-co-glycolide Sheet in Maxillofacial Surgery: An In Vivo Study

Nanomaterials ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 22
Author(s):  
Huy Xuan Ngo ◽  
Quang Ngoc Dong ◽  
Yunpeng Bai ◽  
Jingjing Sha ◽  
Shinji Ishizuka ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Reconstructive Surgery ◽  
Early Stage ◽  
Maxillofacial Surgery ◽  
Male Rats ◽  
Regeneration Ability ◽  
Sprague Dawley ◽  
Micro Computed Tomography ◽  
Rat Mandible

Uncalcined/unsintered hydroxyapatite and poly-l-lactide-co-glycolide (u-HA/PLLA/PGA) is a new bioresorbable nanomaterial with superior characteristics compared with current bioresorbable materials, including appropriate mechanical properties, outstanding bioactive/osteoconductive features, and remarkably shorter resorption time. Nevertheless, the bone regeneration characteristics of this nanomaterial have not been evaluated in maxillofacial reconstructive surgery. In this study, we used a rat mandible model to assess the bone regeneration ability of u-HA/PLLA/PGA material, compared with uncalcined/unsintered hydroxyapatite and poly-l-lactide acid (u-HA/PLLA) material, which has demonstrated excellent bone regenerative ability. A 4-mm-diameter defect was created at the mandibular angle area in 28 Sprague Dawley male rats. The rats were divided into three groups: u-HA/PLLA/PGA (u-HA/PLLA/PGA graft + defect), u-HA/PLLA (u-HA/PLLA graft + defect), and sham control (defect alone). At 1, 3, 8, and 16 weeks after surgeries, the rats were sacrificed and assessed by micro-computed tomography, histological analysis with hematoxylin and eosin staining, and immunohistochemical analyses. The results confirmed that the accelerated bone bioactive/regenerative osteoconduction of u-HA/PLLA/PGA was comparable with that of u-HA/PLLA in the rat mandible model. Furthermore, this new regenerative nanomaterial was able to more rapidly induce bone formation in the early stage and had great potential for further clinical applications in maxillofacial reconstructive surgery.

Sign in / Sign up

Export Citation Format

Share Document