Carboxylated graphene oxide promoted axonal guidance growth by activating Netrin-1/deleted in colorectal cancer signaling in rat primary cultured cortical neurons

2018 ◽  
Vol 106 (6) ◽  
pp. 1500-1510 ◽  
Author(s):  
Meili Liu ◽  
Zhengtai Jia ◽  
Xiongfu Xiao ◽  
Zhifa Zhang ◽  
Ping Li ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Graphene Oxide ◽  
Cortical Neurons ◽  
Axonal Guidance ◽  
Deleted In Colorectal Cancer ◽  
Cancer Signaling ◽  
Cultured Cortical Neurons ◽  
Axonal Guidance Growth

Promotion of Neuronal Guidance Growth by Aminated Graphene Oxide via Netrin-1/Deleted in Colorectal Cancer Signaling

2020 ◽  
Vol 11 (4) ◽  
pp. 604-614
Author(s):  
Meili Liu ◽  
Chongquan Huang ◽  
Zhengtai Jia ◽  
Zhijun Zhao ◽  
Xiongfu Xiao ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Graphene Oxide ◽  
Deleted In Colorectal Cancer ◽  
Cancer Signaling ◽  
Neuronal Guidance

Effect of nano-hydroxyapatite on the axonal guidance growth of rat cortical neurons

Nanoscale ◽  
2012 ◽  
Vol 4 (10) ◽  
pp. 3201 ◽  
Author(s):  
Meili Liu ◽  
Gang Zhou ◽  
Wei Song ◽  
Ping Li ◽  
Haifeng Liu ◽  
...  
Keyword(s):  
Cortical Neurons ◽  
Axonal Guidance ◽  
Rat Cortical Neurons ◽  
Axonal Guidance Growth

scholarly journals The Bacterial Enzyme Cas13 Interferes with Neurite Outgrowth from Cultured Cortical Neurons

Toxins ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 262
Author(s):  
Qin-Wei Wu ◽  
Josef P. Kapfhammer
Keyword(s):  
Rna Editing ◽  
Cortical Neurons ◽  
High Efficiency ◽  
Primary Cultures ◽  
Cultured Neurons ◽  
Therapeutic Tool ◽  
Bacterial Enzyme ◽  
Rna Targeting ◽  
Cultured Cortical Neurons ◽  
New Generation

The CRISPR-Cas13 system based on a bacterial enzyme has been explored as a powerful new method for RNA manipulation. Due to the high efficiency and specificity of RNA editing/interference achieved by this system, it is currently being developed as a new therapeutic tool for the treatment of neurological and other diseases. However, the safety of this new generation of RNA therapies is still unclear. In this study, we constructed a vector expressing CRISPR-Cas13 under a constitutive neuron-specific promoter. CRISPR-Cas13 from Leptotrichia wadei was expressed in primary cultures of mouse cortical neurons. We found that the presence of CRISPR-Cas13 impedes the development of cultured neurons. These results show a neurotoxic action of Cas13 and call for more studies to test for and possibly mitigate the toxic effects of Cas13 enzymes in order to improve CRISPR-Cas13-based tools for RNA targeting.

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