Interplay between self-assembled structure of bone morphogenetic protein-2 (BMP-2) and osteoblast functions in three-dimensional titanium alloy scaffolds: Stimulation of osteogenic activity

2015 ◽  
Vol 104 (2) ◽  
pp. 517-532 ◽  
Author(s):  
K. C. Nune ◽  
A. Kumar ◽  
L. E. Murr ◽  
R. D. K. Misra
Keyword(s):  
Titanium Alloy ◽  
Bone Morphogenetic Protein ◽  
Three Dimensional ◽  
Bone Morphogenetic Protein 2 ◽  
Osteogenic Activity ◽  
Morphogenetic Protein ◽  
Self Assembled ◽  
Stimulation Of

scholarly journals Bone marrow-derived heparan sulfate potentiates the osteogenic activity of bone morphogenetic protein-2 (BMP-2)

Bone ◽  
2012 ◽  
Vol 50 (4) ◽  
pp. 954-964 ◽  
Author(s):  
Diah S. Bramono ◽  
Sadasivam Murali ◽  
Bina Rai ◽  
Ling Ling ◽  
Wei Theng Poh ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Heparan Sulfate ◽  
Bone Morphogenetic Protein ◽  
Bone Morphogenetic Protein 2 ◽  
Osteogenic Activity ◽  
Morphogenetic Protein

scholarly journals Unsaturated Fatty Acids Disrupt Smad Signaling in Gonadotrope Cells Leading to Inhibition of FSHβ Gene Expression

Endocrinology ◽  
2014 ◽  
Vol 155 (2) ◽  
pp. 592-604 ◽  
Author(s):  
Ghislaine Garrel ◽  
Violaine Simon ◽  
Chantal Denoyelle ◽  
Muhammad Ishaq ◽  
Claude Rouch ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Morphogenetic Protein ◽  
Protein Phosphatase ◽  
Unsaturated Fatty Acids ◽  
Reproductive Function ◽  
Bone Morphogenetic Protein 2 ◽  
Pituitary Cells ◽  
Transcript Levels ◽  
Morphogenetic Protein ◽  
Stimulation Of

Reproductive function is highly dependent on nutritional input. We recently provided evidence that the unsaturated ω6 fatty acid (FA), linoleic acid (linoleic), interferes with transcription and secretion of the gonadotropin LH, highlighting the existence of a lipid sensing in pituitary gonadotropes. Here, we show, using a combination of in vivo and in vitro models, that linoleic differentially regulates Lhb and Fshb expression. Central exposure of rats to linoleic over 7 days was associated with increase of Lhb but not Fshb transcript levels. Consistently, exposure of rat pituitary cells or LβT2 cells to linoleic increased Lhb, whereas it dramatically decreased Fshb transcript levels without affecting its stability. This effect was also induced by ω9 and ω3-polyunsaturated FA but not by saturated palmitic acid. Analysis of the underlying mechanisms in LβT2 cells using small interfering RNA revealed that early growth response protein 1 mediates linoleic stimulation of Lhb expression. Furthermore, we demonstrated that linoleic counteracts activin and bone morphogenetic protein-2 stimulation of Fshb expression. Using Western blotting and Smad-responsive reporter gene assays, linoleic was shown to decrease basal Smad2/3 phosphorylation levels as well as activin- and bone morphogenetic protein-2-dependent activation of Smad, uncovering a new FA-sensitive signaling cascade. Finally, the protein phosphatase magnesium-dependent 1A was shown to mediate linoleic inhibition of basal Smad phosphorylation and Fshb expression, identifying protein phosphatase magnesium-dependent 1A as a new target of FA in gonadotropes. Altogether, this study provides a novel mechanism by which FAs target gene expression and underlines the relevant role of pituitary gonadotropes in mediating the effects of nutritional FA on reproductive function.

scholarly journals Stimulation of Bone Healing by Sustained Bone Morphogenetic Protein 2 (BMP-2) Delivery

2014 ◽  
Vol 15 (5) ◽  
pp. 8539-8552 ◽  
Author(s):  
Mirja Faßbender ◽  
Susann Minkwitz ◽  
Catrin Strobel ◽  
Gerhard Schmidmaier ◽  
Britt Wildemann
Keyword(s):  
Bone Morphogenetic Protein ◽  
Bone Healing ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
Stimulation Of
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