scholarly journals Stimulation of Bone Healing by Sustained Bone Morphogenetic Protein 2 (BMP-2) Delivery

2014 ◽  
Vol 15 (5) ◽  
pp. 8539-8552 ◽  
Author(s):  
Mirja Faßbender ◽  
Susann Minkwitz ◽  
Catrin Strobel ◽  
Gerhard Schmidmaier ◽  
Britt Wildemann
Keyword(s):  
Bone Morphogenetic Protein ◽  
Bone Healing ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
Stimulation Of

scholarly journals Bone morphogenetic protein-2-impregnated biomimetic scaffolds successfully induce bone healing in a marginal mandibular defect

2013 ◽  
Vol 123 (5) ◽  
pp. 1149-1155 ◽  
Author(s):  
Adam S. DeConde ◽  
Douglas Sidell ◽  
Min Lee ◽  
Olga Bezouglaia ◽  
Kyle Low ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Bone Healing ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
Biomimetic Scaffolds ◽  
Mandibular Defect

Enhancement of Bone Healing Based onEx VivoGene Therapy Using Human Muscle-Derived Cells Expressing Bone Morphogenetic Protein 2

2002 ◽  
Vol 13 (10) ◽  
pp. 1201-1211 ◽  
Author(s):  
Joon Yung Lee ◽  
Hairong Peng ◽  
Arvydas Usas ◽  
Douglas Musgrave ◽  
James Cummins ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Bone Healing ◽  
Human Muscle ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein

scholarly journals Effect of Bone Morphogenetic Protein-2 in the Treatment of Long Bone Non-Unions

2021 ◽  
Vol 10 (19) ◽  
pp. 4597
Author(s):  
Thomas Fuchs ◽  
Josef Stolberg-Stolberg ◽  
Philipp A. Michel ◽  
Patric Garcia ◽  
Susanne Amler ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Bone Healing ◽  
Treatment Effectiveness ◽  
Patient Characteristics ◽  
Bone Morphogenetic Protein 2 ◽  
Long Bone ◽  
X Rays ◽  
Union Rate ◽  
Morphogenetic Protein ◽  
Significant Difference

Background: Delayed fracture healing continues to cause significant patient morbidity and an economic burden to society. Biological stimulation of non-unions includes application of recombinant bone morphogenetic protein-2 (rhBMP-2). However, rhBMP-2 use continues to be a matter of controversy as literature shows scarce evidence for treatment effectiveness. Questions: The objective of this study was to evaluate the effectiveness of rhBMP-2 treatment on long bone non-unions measuring union rate and time to union. Furthermore, we assess risk factors for treatment failure. Methods and patients: A total of 91 patients with non-unions of long bones were treated with rhBMP-2 (n = 72) or standard care without BMP (n = 19) at our institution. Patient characteristics, comorbidities, nicotine consumption, and complications were recorded. Bone healing was assessed by plane X-rays and clinical examination. Patients were followed up with for 24 months. Results: Overall, there was significantly faster bone healing after rhBMP-2 application compared to the no-BMP group (p < 0.001; HR = 2.78; 95% CI 1.4–5.6). Union rates differed significantly between rhBMP-2 compared to the no-BMP group (89% vs. 47%; p < 0.001). At the humerus, there was neither a significantly higher union rate in the rhBMP-2 (83%) compared to the no-BMP group (50%) (p = 0.26; n = 12) nor a faster bone healing with a median time of 9 months in both groups (HR = 2.01; 95% CI 0.49–8.61; p = 0.315). The 33 femora treated using rhBMP-2 healed significantly faster than 9 femora in the no-BMP group (HR = 2.93; 95% CI 1.00–8.4; p = 0.023) with significant differences in union rate with 85% and 44%, respectively (p = 0.022). Regarding tibia non-unions, 25 out of 27 (93%) healed with a median of 9 months after rhBMP-2 application with no significant difference in the no-BMP group (33%) in time to union (p = 0.097) but a significantly higher union rate (p = 0.039). There was no effect of comorbidities, age, sex, soft tissue damage, or nicotine use on time to union, union rate, or secondary interventions. Conclusion: Consistent with the literature, overall, significantly higher union rates with reduced time to union were achieved after rhBMP-2 application. Femoral and tibial non-unions in particular seem to profit from rhBMP-2 application.

scholarly journals Unsaturated Fatty Acids Disrupt Smad Signaling in Gonadotrope Cells Leading to Inhibition of FSHβ Gene Expression

Endocrinology ◽  
2014 ◽  
Vol 155 (2) ◽  
pp. 592-604 ◽  
Author(s):  
Ghislaine Garrel ◽  
Violaine Simon ◽  
Chantal Denoyelle ◽  
Muhammad Ishaq ◽  
Claude Rouch ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Morphogenetic Protein ◽  
Protein Phosphatase ◽  
Unsaturated Fatty Acids ◽  
Reproductive Function ◽  
Bone Morphogenetic Protein 2 ◽  
Pituitary Cells ◽  
Transcript Levels ◽  
Morphogenetic Protein ◽  
Stimulation Of

Reproductive function is highly dependent on nutritional input. We recently provided evidence that the unsaturated ω6 fatty acid (FA), linoleic acid (linoleic), interferes with transcription and secretion of the gonadotropin LH, highlighting the existence of a lipid sensing in pituitary gonadotropes. Here, we show, using a combination of in vivo and in vitro models, that linoleic differentially regulates Lhb and Fshb expression. Central exposure of rats to linoleic over 7 days was associated with increase of Lhb but not Fshb transcript levels. Consistently, exposure of rat pituitary cells or LβT2 cells to linoleic increased Lhb, whereas it dramatically decreased Fshb transcript levels without affecting its stability. This effect was also induced by ω9 and ω3-polyunsaturated FA but not by saturated palmitic acid. Analysis of the underlying mechanisms in LβT2 cells using small interfering RNA revealed that early growth response protein 1 mediates linoleic stimulation of Lhb expression. Furthermore, we demonstrated that linoleic counteracts activin and bone morphogenetic protein-2 stimulation of Fshb expression. Using Western blotting and Smad-responsive reporter gene assays, linoleic was shown to decrease basal Smad2/3 phosphorylation levels as well as activin- and bone morphogenetic protein-2-dependent activation of Smad, uncovering a new FA-sensitive signaling cascade. Finally, the protein phosphatase magnesium-dependent 1A was shown to mediate linoleic inhibition of basal Smad phosphorylation and Fshb expression, identifying protein phosphatase magnesium-dependent 1A as a new target of FA in gonadotropes. Altogether, this study provides a novel mechanism by which FAs target gene expression and underlines the relevant role of pituitary gonadotropes in mediating the effects of nutritional FA on reproductive function.

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