Development of full-thickness human skin equivalents with blood and lymph-like capillary networks by cell coating technology

2015 ◽  
Vol 103 (10) ◽  
pp. 3386-3396 ◽  
Author(s):  
Michiya Matsusaki ◽  
Kumiko Fujimoto ◽  
Yuji Shirakata ◽  
Satoshi Hirakawa ◽  
Koji Hashimoto ◽  
...  
Keyword(s):  
Human Skin ◽  
Full Thickness ◽  
Coating Technology ◽  
Capillary Networks ◽  
Skin Equivalents ◽  
Cell Coating

FibriDerm: Interpenetrated Fibrin Scaffolds for the Construction of Human Skin Equivalents for Full Thickness Burns

IRBM ◽  
2018 ◽  
Vol 39 (2) ◽  
pp. 103-108 ◽  
Author(s):  
O. Gsib ◽  
M. Deneufchatel ◽  
M. Goczkowski ◽  
M. Trouillas ◽  
M. Resche-Guigon ◽  
...  
Keyword(s):  
Human Skin ◽  
Full Thickness ◽  
Skin Equivalents

scholarly journals 097 Incorporation of chitosan in the dermal matrix improves epidermal barrier formation in full thickness human skin equivalents

2016 ◽  
Vol 136 (9) ◽  
pp. S177
Author(s):  
A. Mieremet ◽  
M. Rietveld ◽  
S. Absalah ◽  
Jv Smeden ◽  
J. Bouwstra ◽  
...  
Keyword(s):  
Human Skin ◽  
Full Thickness ◽  
Dermal Matrix ◽  
Epidermal Barrier ◽  
Skin Equivalents ◽  
Barrier Formation

CCL5 and CCL20 mediate immigration of Langerhans cells into the epidermis of full thickness human skin equivalents

2012 ◽  
Vol 91 (10) ◽  
pp. 765-773 ◽  
Author(s):  
Krista Ouwehand ◽  
Sander W. Spiekstra ◽  
Taco Waaijman ◽  
Melanie Breetveld ◽  
Rik J. Scheper ◽  
...  
Keyword(s):  
Human Skin ◽  
Langerhans Cells ◽  
Full Thickness ◽  
Skin Equivalents

scholarly journals 3D biomaterial matrix to support long term, full thickness, immuno-competent human skin equivalents with nervous system components

Biomaterials ◽  
2019 ◽  
Vol 198 ◽  
pp. 194-203 ◽  
Author(s):  
Sarah E. Lightfoot Vidal ◽  
Kasey A. Tamamoto ◽  
Hanh Nguyen ◽  
Rosalyn D. Abbott ◽  
Dana M. Cairns ◽  
...  
Keyword(s):  
Nervous System ◽  
Human Skin ◽  
Full Thickness ◽  
Skin Equivalents ◽  
System Components

scholarly journals Mechanical and Immunological Regulation in Wound Healing and Skin Reconstruction

2021 ◽  
Vol 22 (11) ◽  
pp. 5474
Author(s):  
Shun Kimura ◽  
Takashi Tsuji
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Human Skin ◽  
Developmental Stages ◽  
Control Mechanisms ◽  
Tissue Remodelling ◽  
Skin Equivalents ◽  
Immunological Interactions ◽  
Stress Signals ◽  
Specific Species

In the past decade, a new frontier in scarless wound healing has arisen because of significant advances in the field of wound healing realised by incorporating emerging concepts from mechanobiology and immunology. The complete integumentary organ system (IOS) regeneration and scarless wound healing mechanism, which occurs in specific species, body sites and developmental stages, clearly shows that mechanical stress signals and immune responses play important roles in determining the wound healing mode. Advances in tissue engineering technology have led to the production of novel human skin equivalents and organoids that reproduce cell–cell interactions with tissue-scale tensional homeostasis, and enable us to evaluate skin tissue morphology, functionality, drug response and wound healing. This breakthrough in tissue engineering has the potential to accelerate the understanding of wound healing control mechanisms through complex mechanobiological and immunological interactions. In this review, we present an overview of recent studies of biomechanical and immunological wound healing and tissue remodelling mechanisms through comparisons of species- and developmental stage-dependent wound healing mechanisms. We also discuss the possibility of elucidating the control mechanism of wound healing involving mechanobiological and immunological interaction by using next-generation human skin equivalents.

scholarly journals Effect of α-Lipoic Acid on the Development of Human Skin Equivalents Using a Pumpless Skin-on-a-Chip Model

2021 ◽  
Vol 22 (4) ◽  
pp. 2160
Author(s):  
Kyunghee Kim ◽  
Jisue Kim ◽  
Hyoungseob Kim ◽  
Gun Yong Sung
Keyword(s):  
Gene Expression ◽  
Human Skin ◽  
Lipoic Acid ◽  
Drug Testing ◽  
Structural Integrity ◽  
Microfluidic Channel ◽  
Skin Barrier Function ◽  
Animal Testing ◽  
Epidermal Structure ◽  
Skin Equivalents

Owing to the prohibition of cosmetic animal testing, various attempts have recently been made using skin-on-a-chip (SOC) technology as a replacement for animal testing. Previously, we reported the development of a pumpless SOC capable of drug testing with a simple drive using the principle that the medium flows along the channel by gravity when the chip is tilted using a microfluidic channel. In this study, using pumpless SOC, instead of drug testing at the single-cell level, we evaluated the efficacy of α-lipoic acid (ALA), which is known as an anti-aging substance in skin equivalents, for skin tissue and epidermal structure formation. The expression of proteins and changes in genotyping were compared and evaluated. Hematoxylin and eosin staining for histological analysis showed a difference in the activity of fibroblasts in the dermis layer with respect to the presence or absence of ALA. We observed that the epidermis layer became increasingly prominent as the culture period was extended by treatment with 10 μM ALA. The expression of epidermal structural proteins of filaggrin, involucrin, keratin 10, and collagen IV increased because of the effect of ALA. Changes in the epidermis layer were noticeable after the ALA treatment. As a result of aging, damage to the skin-barrier function and structural integrity is reduced, indicating that ALA has an anti-aging effect. We performed a gene analysis of filaggrin, involucrin, keratin 10, integrin, and collagen I genes in ALA-treated human skin equivalents, which indicated an increase in filaggrin gene expression after ALA treatment. These results indicate that pumpless SOC can be used as an in vitro skin model similar to human skin, protein and gene expression can be analyzed, and it can be used for functional drug tests of cosmetic materials in the future. This technology is expected to contribute to the development of skin disease models.

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