Local delivery of COMP-angiopoietin 1 accelerates new bone formation in rat calvarial defects

2015 ◽  
Vol 103 (9) ◽  
pp. 2942-2951 ◽  
Author(s):  
Shin-Saeng Lim ◽  
Sung-Ho Kook ◽  
Govinda Bhattarai ◽  
Eui-Sic Cho ◽  
Young-Kwon Seo ◽  
...  
Keyword(s):  
Bone Formation ◽  
Local Delivery ◽  
New Bone Formation ◽  
Calvarial Defects ◽  
Angiopoietin 1

Effect of Particle Size on New Bone Formation In Vivo Using Calcium Phopshate Glass

2007 ◽  
Vol 330-332 ◽  
pp. 165-168
Author(s):  
Hyun Ju Moon ◽  
Racquel Z. LeGeros ◽  
Kyoung Nam Kim ◽  
Kwang Mahn Kim ◽  
Seong Ho Choi ◽  
...  
Keyword(s):  
Particle Size ◽  
Calcium Phosphate ◽  
Bone Formation ◽  
Phosphate Glass ◽  
Glass Powder ◽  
New Bone Formation ◽  
Sprague Dawley ◽  
Constant Weight ◽  
Calvarial Defects

The purpose of this study was to compare the bone regenerative effect of calcium phosphate glass according to the particle size in vivo. We prepared two different sizes, that is 400 μm and 40 μm, of calcium phosphate glass powder using the system CaO-CaF2-P2O5-MgO-ZnO. Critical-sized calvarial defects were created in 60 male Sprague-Dawley rats. The animals were divided into 3 groups of 20 animals each. Each defect was filled with a constant weight of 0.5 g calcium phosphate glass powder mixed with saline. As controls, the defect was left empty. The rats were sacrificed 2 or 8 weeks after postsurgery, and the results were evaluated using histological as well as histomorphometrical studies. The particle size of the calcium phosphate was crucial; 400 μm particles promoted new bone formation, while 40 μm particles inhibited it because of severe inflammation.

scholarly journals In Vivo Bone Regeneration Induced by a Scaffold of Chitosan/Dicarboxylic Acid Seeded with Human Periodontal Ligament Cells

2019 ◽  
Vol 20 (19) ◽  
pp. 4883 ◽  
Author(s):  
Teerawat Sukpaita ◽  
Suwabun Chirachanchai ◽  
Pornchanok Suwattanachai ◽  
Vincent Everts ◽  
Atiphan Pimkhaokham ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Formation ◽  
Dicarboxylic Acid ◽  
Bone Regeneration ◽  
Periodontal Ligament ◽  
Periodontal Ligament Cells ◽  
Micro Ct ◽  
New Bone Formation ◽  
Calvarial Defects

Chitosan/dicarboxylic acid (CS/DA) scaffold has been developed as a bone tissue engineering material. This study evaluated a CS/DA scaffold with and without seeded primary human periodontal ligament cells (hPDLCs) in its capacity to regenerate bone in calvarial defects of mice. The osteogenic differentiation of hPDLCs was analyzed by bone nodule formation and gene expression. In vivo bone regeneration was analyzed in mice calvarial defects. Eighteen mice were divided into 3 groups: one group with empty defects, one group with defects with CS/DA scaffold, and a group with defects with CS/DA scaffold and with hPDLCs. After 6 and 12 weeks, new bone formation was assessed using microcomputed tomography (Micro-CT) and histology. CS/DA scaffold significantly promoted in vitro osteoblast-related gene expression (RUNX2, OSX, COL1, ALP, and OPN) by hPDLCs. Micro-CT revealed that CS/DA scaffolds significantly promoted in vivo bone regeneration both after 6 and 12 weeks (p < 0.05). Histological examination confirmed these findings. New bone formation was observed in defects with CS/DA scaffold; being similar with and without hPDLCs. CS/DA scaffolds can be used as a bone regenerative material with good osteoinductive/osteoconductive properties.

Anorganic Bone Mineral Coated with Tetra-Cell Adhesion Molecule Enhances Bone Formation in Rabbit Calvarial Defects

2006 ◽  
Vol 309-311 ◽  
pp. 981-984
Author(s):  
Ji Ho Lee ◽  
Jin Woo Park ◽  
Byung Ju Choi ◽  
In San Kim ◽  
Jo Young Suh
Keyword(s):  
Cell Adhesion ◽  
Bone Formation ◽  
Bone Mineral ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Statistical Significance ◽  
Type I ◽  
Graft Material ◽  
New Bone Formation ◽  
Calvarial Defects

This study was performed to evaluate the effect of anorganic bone mineral (ABM) coated with Tetra-Cell Adhesion Molecule (T-CAM) for bone formation in rabbit calvarial defects and compare the capability of bone formation in ABM coated with T-CAM (ABM/T-CAM) to that in commercially available ABM coated with a synthetic peptide (P-15) which mimics the cell-binding domain of type I collagen, PepGen P-15TM. T-CAM composed of four cell adhesion molecules (RGD, PHSRN, EPDIM, and YH) was synthesized and ABM/T-CAM were prepared by absorbing T-CAM on ABM (OsteoGraf/N-300; Densply Friadent Ceramed Corp., USA). Two 9-mm diameter, full-thickness calvarial defects were made in each rabbit parietal bone and sixteen adult male rabbits were used in this experiment. The defects were reconstructed according to four treatment groups: unfilled, BM-grafted, PepGen P-15TM-grafted, and ABM/T-CAM-grafted. The animals were sacrificed at 2 and 4 weeks after surgery for histologic and histomorphometric evaluation. An active new bone formation were observed in the defects of ABM/T-CAM and PepGen P-15TM grafted groups at 2 and 4 weeks of healing in histologic observation. The results of histomorphometric analysis revealed higher new bone formation in ABM/T-CAM-grafted (14.62±0.6% at 2 weeks, 15.33±2.4% at 4 weeks) and PepGen P-15TM-grafted (12.46±1.0% at 2 weeks, 18.14±1.7% at 4 weeks) groups than in unfilled control (7.03±2.3% at 2 weeks, 8.71±3.4% at 4 weeks) and ABMgrafted (6.59±1.7% at 2 weeks, 9.25±0.8% at 4 weeks) groups at 2 and 4 weeks of healing with statistical significance (P<0.01). The results of this study indicated that the immobilizing T-CAM on ABM enhances the capability of bone substitutes to serve as an effective habitat for bone forming cells in vivo. In conclusion, we suggested that this composite graft material, ABM/T-CAM may be served as an effective tissue-engineered bone graft material in osseous reconstructive surgery.

Evaluation of New Bone Formation Using Autogenous Tooth Bone Graft Combined with Platelet-Rich Fibrin in Calvarial Defects

2019 ◽  
Vol 30 (6) ◽  
pp. 1662-1666 ◽  
Author(s):  
Alper Kizildağ ◽  
Ufuk Taşdemir ◽  
Taner Arabaci ◽  
Özlem Özmen ◽  
Canan Aksu Kizildağ ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Graft ◽  
New Bone Formation ◽  
Platelet Rich Fibrin ◽  
Calvarial Defects

scholarly journals Evaluation of Open Hollow Hydroxyapatite Microsphere on Bone Regeneration in Rat Calvarial Defects

2019 ◽  
Author(s):  
Youqu Shen ◽  
Mohamed Rahaman ◽  
Yongxian Liu ◽  
Yue-Wern Huang
Keyword(s):  
Surface Roughness ◽  
Bone Formation ◽  
Bone Regeneration ◽  
Convex Surface ◽  
Total Surface Area ◽  
Calvarial Defect ◽  
New Bone Formation ◽  
Calvarial Defects ◽  
The Difference ◽  
Rat Calvarial Defect

AbstractHollow hydroxyapatite (HA) microspheres showed the ability to facilitate bone regeneration in rats with non-healing calvarial defects. However, new bone formation in the rat calvarial defect implanted with the closed HA microspheres was limited. The objective of this work is to evaluate size-, time, and structure-dependent bone regeneration between open and closed HA microspheres in an osseous model. Open HA microspheres were obtained by sectioning closed HA microspheres. The open HA microsphere had dense convex surface and rough and porous concave surface. For both size ranges (ϕ106-150 μm vs. ϕ212-250 μm), the open HA microsphere were more effective in facilitating bone regeneration than the closed HA microspheres in rat calvarial defects. Bone regeneration in the open HA microspheres (49 ± 7% for ϕ106-150 μm; 40 ± 8% for ϕ212-250 μm) were higher than the closed HA microsphere (26 ± 8% for ϕ106-150 μm; 30 ± 9% for ϕ212-250 μm) at 12 weeks. Furthermore, the open HA microspheres of smaller size showed a significant increase in bone regeneration than the open HA microspheres of larger size at both 6 weeks and 12 weeks. The difference in bone regeneration between these microspheres could be due to their differences in microstructures, namely curvature, concavity, porosity, surface roughness, and total surface area available for cells to attached to.

The effect of local delivery of adiponectin from biodegradable microsphere–scaffold composites on new bone formation in adiponectin knockout mice

2016 ◽  
Vol 4 (27) ◽  
pp. 4771-4779 ◽  
Author(s):  
Dan Li ◽  
Yuan Guo ◽  
Hui Lu ◽  
Ren Wang ◽  
Hong-cheng Hu ◽  
...  
Keyword(s):  
Bone Formation ◽  
Knockout Mice ◽  
Pi3k Pathway ◽  
Local Delivery ◽  
Osteoblast Proliferation ◽  
New Bone Formation ◽  
Increase Bone Formation

Adiponectin (APN) is the most abundant adipocyte-secreted adipokine; it increase bone formation partially by promoting osteoblast proliferationviathe APPL1/PI3K pathway.

Microtomography analysis of bone formation in calvarial defects filled with gelatin sponge or fibrin glue

2013 ◽  
Author(s):  
Santana Santos Thiago de ◽  
Helena Bacha Lopes ◽  
Adriana Luiza Almeida ◽  
Marcio Mateus Beloti ◽  
Adalberto Luiz Rosa
Keyword(s):  
Bone Formation ◽  
Fibrin Glue ◽  
Gelatin Sponge ◽  
Calvarial Defects
Sign in / Sign up

Export Citation Format

Share Document