Influence of nanoparticle-embedded polymeric surfaces on cellular adhesion, proliferation, and differentiation

2013 ◽  
Vol 102 (8) ◽  
pp. 2652-2661 ◽  
Author(s):  
Letizia Ventrelli ◽  
Toshinori Fujie ◽  
Serena Del Turco ◽  
Giuseppina Basta ◽  
Barbara Mazzolai ◽  
...  
Keyword(s):  
Cellular Adhesion ◽  
Polymeric Surfaces ◽  
Proliferation And Differentiation

scholarly journals Engineering Cell Adhesion and Orientation via Ultrafast Laser Fabricated Microstructured Substrates

2018 ◽  
Vol 19 (7) ◽  
pp. 2053 ◽  
Author(s):  
Eleftheria Babaliari ◽  
Paraskevi Kavatzikidou ◽  
Despoina Angelaki ◽  
Lefki Chaniotaki ◽  
Alexandra Manousaki ◽  
...  
Keyword(s):  
Soft Lithography ◽  
Cellular Adhesion ◽  
Cell Alignment ◽  
Cell Responses ◽  
Axonal Regrowth ◽  
Proliferation And Differentiation ◽  
Polymeric Substrates ◽  
Extracellular Environment

Cell responses depend on the stimuli received by the surrounding extracellular environment, which provides the cues required for adhesion, orientation, proliferation, and differentiation at the micro and the nano scales. In this study, discontinuous microcones on silicon (Si) and continuous microgrooves on polyethylene terephthalate (PET) substrates were fabricated via ultrashort pulsed laser irradiation at various fluences, resulting in microstructures with different magnitudes of roughness and varying geometrical characteristics. The topographical models attained were specifically developed to imitate the guidance and alignment of Schwann cells for the oriented axonal regrowth that occurs in nerve regeneration. At the same time, positive replicas of the silicon microstructures were successfully reproduced via soft lithography on the biodegradable polymer poly(lactide-co-glycolide) (PLGA). The anisotropic continuous (PET) and discontinuous (PLGA replicas) microstructured polymeric substrates were assessed in terms of their influence on Schwann cell responses. It is shown that the micropatterned substrates enable control over cellular adhesion, proliferation, and orientation, and are thus useful to engineer cell alignment in vitro. This property is potentially useful in the fields of neural tissue engineering and for dynamic microenvironment systems that simulate in vivo conditions.

scholarly journals Engineering Cell Adhesion and Orientation via Ultrafast Laser Fabricated Microstructured Substrates

2018 ◽  
Author(s):  
Eleftheria Babaliari ◽  
Paraskevi Kavatzikidou ◽  
Despoina Angelaki ◽  
Lefki Chaniotaki ◽  
Alexandra Manousaki ◽  
...  
Keyword(s):  
Schwann Cells ◽  
Soft Lithography ◽  
Cellular Adhesion ◽  
Cell Alignment ◽  
Axonal Regrowth ◽  
Proliferation And Differentiation ◽  
Polymeric Substrates ◽  
Extracellular Environment

Cells take decisions on their responses depending on the stimuli received by the surrounding extracellular environment, that provides the essential cues at the micro and the nano-lengthscales required for adhesion, orientation, proliferation and differentiation. In this study, discontinuous microcones on silicon (Si) and continuous microgrooves on polyethylene terephthalate (PET) substrates were fabricated via ultrashort-pulsed laser irradiation at various fluences, resulting in microstructures with different roughness and geometrical characteristics. The topographical models attained were specifically developed to imitate the guidance and alignment of Schwann cells for oriented axonal regrowth, towards nerve regeneration. At the same time, positive replicas of the silicon microstructures formed were successfully reproduced, via soft lithography, on the biodegradable polymer poly(lactide-co-glycolide) (PLGA). The anisotropic continuous (PET) and discontinuous (PLGA replicas) microstructured polymeric substrates were assessed in terms of their influence on the Schwann cells responses. It is shown that the developed micropatterned substrates enable control over the cellular adhesion, proliferation and orientation and are thus useful to engineer cell alignment in vitro. This property could be potentially useful in the fields of neural tissue engineering and for dynamic microenvironment systems that simulate in vivo conditions.

Osteoblast Adhesion on Tissue Engineering Scaffolds Made by Bio-Manufacturing Techniques

2005 ◽  
Author(s):  
T. Dutta Roy ◽  
J. J. Stone ◽  
W. Sun ◽  
E. H. Cho ◽  
S. J. Lockett ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Focal Adhesion ◽  
Cellular Response ◽  
Focal Adhesions ◽  
Cellular Adhesion ◽  
Tissue Engineering Scaffolds ◽  
3D Scaffolds ◽  
Imaging Results ◽  
Proliferation And Differentiation ◽  
Manufacturing Techniques

Scientific exploration into understanding and developing relationships between three-dimensional (3D) scaffolds prepared by rapid prototyping (RP) and cellular response has focused primarily on end results targeting osteoblast proliferation and differentiation. Here at the National Institute of Standards and Technology (NIST), we take a systems approach to developing relationships between material properties and quantitative biological responses. This study in particular focuses on the screening of parameters controlled by RP techniques and their ability to trigger signalling events leading to cell adhesion. This pioneering research in our group also characterizes the in vitro cell-material interactions of 2D films and 3D scaffolds. From there, one can postulate on contributory factors leading to cell migration, proliferation, and differentiation. In summary, we believe that the quantitative information from this fundamental investigation will enhance our knowledge of the interactions between cells and 3D material interfaces with respect to formation of focal adhesions. This work consists of two sections — the application of imaging techniques for 3D characterization of properties and culturing of osteoblasts for size and shape determination. This includes quantifying the number of focal adhesion sites. We are using 3D RP polycaprolactone (PCL) scaffolds as this surrogate model in which to compare 2D to 3D material performance and cell interactions. Using RP bio-manufacturing techniques to fabricate tissue engineering scaffolds allows for control of pore size, strut size, and layer thickness, therefore providing adjustable parameters to study which can potentially influence, or even dynamically modulate, cellular adhesion. Imaging results after culturing for 24 h showed differences in cell morphology and spreading relative to the different structures. The focal adhesion response also varied, indicating an apparent loss of organization in 3D scaffolds compared to 2D surfaces. See Results and Discussion for details.

Osteoblast adhesion, proliferation and growth on polyelectrolyte complex–hydroxyapatite nanocomposites

2010 ◽  
Vol 368 (1917) ◽  
pp. 2083-2097 ◽  
Author(s):  
Devendra Verma ◽  
Kalpana S. Katti ◽  
Dinesh R. Katti
Keyword(s):  
Mechanical Response ◽  
Three Dimensional ◽  
Staining Technique ◽  
Cellular Adhesion ◽  
Nanocomposite Films ◽  
Drying Methods ◽  
Alizarin Red ◽  
Optical Images ◽  
Proliferation And Differentiation ◽  
Osteoblast Adhesion

In this work, we have investigated osteoblast adhesion, proliferation and differentiation on nanocomposites of chitosan, polygalacturonic acid (PgA) and hydroxyapatite. These studies were done on both two- and three-dimensional (scaffold) samples. Atomic force microscopy experiments showed nanostructuring of film samples. Scaffolds were prepared by freeze-drying methods. The mechanical response and porosity of the scaffolds were also determined. The compressive elastic modulus and compressive strength were determined to be around 0.9 and 0.023 MPa, respectively, and the porosity of these scaffolds was found to be around 97 per cent. Human osteoblast cells were used to study their adhesion, proliferation and differentiation. Optical images were collected after different intervals of time of seeding cells. This study indicated that chitosan/PgA/hydroxyapatite nanocomposite films and scaffolds promote cellular adhesion, proliferation and differentiation. The formation of bone-like nodules was observed after 7 days of seeding cells. The nodule size continues to increase with time, and after 20 days the size of some nodules was around 735 μm. Scanning electron microscope images of nodules showed the presence of extracellular matrix. The alizarin red S staining technique was used to confirm mineralization of these nodules.

scholarly journals Biological Effects of Polyrotaxane Surfaces on Cellular Responses of Fibroblast, Preosteoblast and Preadipocyte Cell Lines

Polymers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 924
Author(s):  
Hiroki Masuda ◽  
Yoshinori Arisaka ◽  
Ruriko Sekiya-Aoyama ◽  
Tetsuya Yoda ◽  
Nobuhiko Yui
Keyword(s):  
Cellular Proliferation ◽  
Biological Effects ◽  
Cellular Responses ◽  
Structural Features ◽  
Cellular Adhesion ◽  
Cell Type ◽  
Poly Ethylene Glycol ◽  
Tissue Culture Polystyrene ◽  
Proliferation And Differentiation ◽  
Poly Ethylene

Biointerfaces based on polyrotaxane (PRX), consisting of α-cyclodextrins (α-CDs) threaded on a poly(ethylene glycol) (PEG) chain, are promising functionalized platforms for culturing cells. PRXs are characterized by the molecular mobility of constituent molecules where the threading α-CDs can move and rotate along the PEG chain. Taking advantage of this mobility, we have previously succeeded in demonstrating the regulation of cellular responses, such as cellular adhesion, proliferation, and differentiation. In the present study, we investigated differences in the cellular responses to PRX surfaces versus commercially available tissue culture polystyrene (TCPS) surfaces using fibroblasts, preosteoblasts, and preadipocytes. PRX surfaces were found to more significantly promote cellular proliferation than the TCPS surfaces, regardless of the cell type. To identify the signaling pathways involved in the activation of cellular proliferation, a DNA microarray analysis was performed. PRX surfaces showed a significant increase in the integrin-mediated cell adhesion and focal adhesion pathways. Furthermore, PRX surfaces also promoted osteoblast differentiation more than TCPS. These results suggest that structural features of PRX surfaces act as mechanical cues to dominate cellular proliferation and differentiation.

Preparation of Collagen/Hydroxyapatite Composite Coating on Porous Titanium Substrate and its Cellular Response

2015 ◽  
Vol 815 ◽  
pp. 429-433
Author(s):  
Ting Li ◽  
Jin Shan Li ◽  
Hong Chao Kou ◽  
Fu Ping Li ◽  
Ting Li Lu
Keyword(s):  
Composite Coating ◽  
Cellular Response ◽  
Energy Dispersive Spectrometer ◽  
Titanium Substrate ◽  
Porous Titanium ◽  
Organic Coating ◽  
Cellular Adhesion ◽  
Cellular Viability ◽  
Hydroxyapatite Composite ◽  
Proliferation And Differentiation

In this article, the layered deposition method is adopted to prepare the collagen/hydroxyapatite (COL/HA) composite coating. The morphology and elements of the COL/HA composite coating are observed using a scanning electron microscope (SEM) with an energy dispersive spectrometer (EDS), the optical density (O.D.) values are obtained by MTT assay to assess the cellular viabilityof composite coating. The experimental results showed that the addition of collagen not only improve the bonding strength of composite coating and porous titanium substrate, but also combine the osteoconduction of inorganic coating HA and the osteoinduction of organic coating COL, effectively enhance the cellular adhesion, proliferation and differentiation. The cellular viability cultured in COL/HA composite coating is much higher than the pure HA modified coating.

scholarly journals β-Catenin immunocytochemical reactivity in cervicovaginal smears during regular menstrual cycles

2020 ◽  
Vol 14 (5) ◽  
pp. 187-194 ◽  
Author(s):  
Hanife Guler Donmez
Keyword(s):  
Epithelial Cells ◽  
Cellular Adhesion ◽  
Secretory Phase ◽  
Signaling Mechanism ◽  
Menstrual Cycles ◽  
Proliferative Phase ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
The Mean ◽  
Cycle Dependent

AbstractBackgroundβ-Catenin mediates cellular adhesion and the Wnt/β-catenin signaling mechanism, thereby controlling cell proliferation and differentiation. Studies of endometrial tissue suggest that there are differences in β-catenin expression during the course of regular menstrual cycles. However, differences in expression in squamous epithelial cells between the proliferative and secretory phases have hitherto remained unknown.ObjectivesTo localize β-catenin in squamous epithelial cells in cervicovaginal smears during the course of regular menstrual cycles.MethodsIn this observational study, smears were taken from women (n = 102) with various gynecological complaints. Squamous epithelial cells were stained using a Papanicolaou method to evaluate their cytology and any infection. An anti-β-catenin antibody was used to localize immunoreactivity in the cell membrane, cytoplasm, and/or nucleus.ResultsWomen with a regular menstrual cycle (n = 62) were divided into 2 groups: those in a proliferative phase (26/62, 42%) and those in a secretory phase (36/62, 58%). Cytoplasmic and nuclear β-catenin immunoreactivity was observed prominently in the proliferative phase (19/26, 73%), whereas low-level β-catenin immunoreactivity was seen in the secretory phase (9/36, 25%). Compared with the secretory phase, the mean H-scores for β-catenin immunoreactivity in the proliferative phase were significantly increased in the membrane (P = 0.039), the cytoplasm (P < 0.001), and the nucleus (P = 0.033). By contrast, β-catenin immunoreactivity was reduced from parabasal to superficial cells in both the proliferative and secretory phases.ConclusionsCytoplasmic and/or nuclear β-catenin immunoreactivity may indicate that the activity of the Wnt/β-catenin signaling pathway is cycle dependent.

scholarly journals A Review of the Effect of a Nanostructured Thin Film Formed by Titanium Carbide and Titanium Oxides Clustered around Carbon in Graphitic Form on Osseointegration

Nanomaterials ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 1233 ◽  
Author(s):  
Roberto Scandurra ◽  
Anna Scotto d’Abusco ◽  
Giovanni Longo
Keyword(s):  
Titanium Carbide ◽  
Biological Tissues ◽  
Cellular Adhesion ◽  
Titanium Implants ◽  
Nanostructured Coating ◽  
Titanium Oxides ◽  
Nanostructured Thin Film ◽  
Proliferation And Differentiation ◽  
Good Biocompatibility

Improving the biocompatibility of implants is an extremely important step towards improving their quality. In this review, we recount the technological and biological process for coating implants with thin films enriched in titanium carbide (TiC), which provide improved cell growth and osseointegration. At first, we discuss the use of a Pulsed Laser Ablation Deposition, which produced films with a good biocompatibility, cellular stimulation and osseointegration. We then describe how Ion Plating Plasma Assisted technology could be used to produce a nanostructured layer composed by graphitic carbon, whose biocompatibility is enhanced by titanium oxides and titanium carbide. In both cases, the nanostructured coating was compact and strongly bound to the bulk titanium, thus particularly useful to protect implants from the harsh oxidizing environment of biological tissues. The morphology and chemistry of the nanostructured coating were particularly desirable for osteoblasts, resulting in improved proliferation and differentiation. The cellular adhesion to the TiC-coated substrates was much stronger than to uncoated surfaces, and the number of philopodia and lamellipodia developed by the cells grown on the TiC-coated samples was higher. Finally, tests performed on rabbits confirmed in vivo that the osseointegration process of the TiC-coated implants is more efficient than that of uncoated titanium implants.

Segregation of cell adhesion molecules into membrane microdomains facilitates leukocyte-endothelial interactions

1995 ◽  
Vol 53 ◽  
pp. 780-781
Author(s):  
S.L. Erlandsen
Keyword(s):  
Cell Surface ◽  
Adhesion Molecules ◽  
Colloidal Gold ◽  
High Spatial Resolution ◽  
Low Voltage ◽  
Cellular Adhesion ◽  
Membrane Microdomains ◽  
Immunogold Label ◽  
Cellular Adhesion Molecules ◽  
Electron Detection

Cells interact with their extracellular environments by means of a variety of cellular adhesion molecules (CAM) and surface ligands. In many instances, CAMs interact in a sequential temporal fashion which suggests that these adhesion molecules may occupy or be polarized to various membrane microdomains on the cell surface. Detection of CAMs can be accomplished by a variety of methods including immunofluorescent microscopy and flow cytometry, and by the use of immunocytochemical markers (i.e. colloidal gold) in electron microscopy. The development of high resolution field emission SEM in the mid 1980's and the Autrata modification of the YAG detector for backscatter electron detection at low voltage has greatly facilitated the recognition of colloidal gold probes for detection of surface CAMs. Low voltage FESEM with Bse imaging provides increased resolution of cell surface topography (~3nm at 3-4 keV) which can be observed in 3-dimensions, and simultaneously permits detection/high spatial resolution of immunogold label by atomic number contrast.

1428: Influence of Prostatic Epithelium on Proliferation and Differentiation of Stromal Cells

2005 ◽  
Vol 173 (4S) ◽  
pp. 387-387
Author(s):  
Quan Wu ◽  
Jian-Dang Shi ◽  
Yu Liu ◽  
Ke-Ming Wang ◽  
Helmut Klocker ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Proliferation And Differentiation ◽  
Prostatic Epithelium
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