Human fibroblast-derived extracellular matrix constructs for bone tissue engineering applications

2013 ◽  
Vol 101 (10) ◽  
pp. 2826-2837 ◽  
Author(s):  
Gregory Tour ◽  
Mikael Wendel ◽  
Ion Tcacencu
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Human Fibroblast ◽  
Engineering Applications

scholarly journals A Proposed Fabrication Method of Novel PCL-GEL-HAp Nanocomposite Scaffolds for Bone Tissue Engineering Applications

2010 ◽  
Vol 19 (4) ◽  
pp. 096369351001900 ◽  
Author(s):  
A. Hamlekhan ◽  
M. Mozafari ◽  
N. Nezafati ◽  
M. Azami ◽  
H. Hadipour
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Mechanical Test ◽  
Fibroblast Cell ◽  
Mouse Fibroblast ◽  
Fabrication Method ◽  
Engineering Applications ◽  
Poly Caprolactone

In this study, poly(∊-caprolactone) (PCL), gelatin (GEL) and nanocrystalline hydroxyapatite (HAp) was applied to fabricate novel PCL-GEL-HAp nanaocomposite scaffolds through a new fabrication method. With the aim of finding the best fabrication method, after testing different methods and solvents, the best method and solvents were found, and the nanocomposites were prepared through layer solvent casting combined with freeze-drying. Acetone and distillated water were used as the PCL and GEL solvents, respectively. The mechanical test showed that the increasing of the PCL weight through the scaffolds caused the improvement of the final nanocomposite mechanical behavior due to the increasing of the ultimate stress, stiffness and elastic modulus (8 MPa for 0% wt PCL to 23.5 MPa for 50% wt PCL). The biomineralization investigation of the scaffolds revealed the formation of bone-like apatite layers after immersion in simulated body fluid (SBF). In addition, the in vitro cytotoxity of the scaffolds using L929 mouse fibroblast cell line (ATCC) indicated no sign of toxicity. These results indicated that the fabricated scaffold possesses the prerequisites for bone tissue engineering applications.

Multiferroic Reinforced Bioactive Glass Composites for Bone Tissue Engineering Applications

2018 ◽  
Vol 20 (12) ◽  
pp. 1800329 ◽  
Author(s):  
Chandra Khatua ◽  
Dipten Bhattacharya ◽  
Biswanath Kundu ◽  
Vamsi Krishna Balla ◽  
Subhadip Bodhak ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Engineering Applications ◽  
Glass Composites

scholarly journals Cell-secreted extracellular matrix, independent of cell source, promotes the osteogenic differentiation of human stromal vascular fraction

2018 ◽  
Vol 6 (24) ◽  
pp. 4104-4115 ◽  
Author(s):  
Jenna N. Harvestine ◽  
Hakan Orbay ◽  
Jonathan Y. Chen ◽  
David E. Sahar ◽  
J. Kent Leach
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Bone Tissue Engineering ◽  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Stromal Vascular Fraction ◽  
Cell Source

Cell-secreted extracellular matrix potentiates osteogenic differentiation by stromal vascular fraction for bone tissue engineering.

Nano Hydroxyapatite (Nano-Hap): A Potential Bioceramic For Biomedical Applications

2021 ◽  
Vol 06 ◽  
Author(s):  
Varun Saxena ◽  
Lalit Pandey ◽  
T. S. Srivatsan
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biomedical Applications ◽  
High Surface ◽  
High Surface Area ◽  
Biological Properties ◽  
Engineering Applications ◽  
Biological Responses ◽  
Size And Morphology

Background: Hydroxyapatite (HAp) is one of the most studied biomimic for biomedical applications. Specially, nano-HAp has been utilized for bone tissue engineering various orthopedic applications. HAp possesses various suitable properties such as bioactivity, biodegradability and cell proliferation efficiency for bone tissue engineering applications. Yet, lacks in self-antibacterial activity, high surface area and target efficiency. Results: In this directioon, researchers have focused on exploring the required surface as well as the inherent properties of HAp at the nanoscale. These properties are largely dependent on the composition, size and morphology of the nano-HAp. Hence, nano-HAp has been found to be an excellent candidate with an attractive combination of properties for selection and use in biomedical applications, those required to enhanced biological responses. Further, depending on the type of application, these factors can be tuned to optimize the performance. Conclusion: In this review article, we focus on the chemical structure of HAp and the routes chosen and used for the synthesis of the nano-HAp. The role of various parameters in controlling synthesis at the nanoscale are presented and briefly discussed. In addition, we provide an overview of the various applications for the pristine and doped nano-HAp with recent examples in areas spanning the following: (i) bone tissue engineering applications, (ii) drug delivery applications, (iii) surface coatings, and (iv) scaffolds. The effect of chemical composition on the mechanical properties, surface properties and biological properties are also highlighted. Nano-HAp is found to be highly proficient for its biomedical applications, especially for bone tissue engineering applications. The nano-sized properties enhances the biological responses. The dopant ions that replaces the Ca ion into the hydroxyapatite (HAp) lattice plays a crucial role in its biomedical applications

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