The impact of immobilization of BMP-2 on PDO membrane for bone regeneration

2012 ◽  
Vol 100A (6) ◽  
pp. 1488-1493 ◽  
Author(s):  
Ji-Eun Kim ◽  
Eun-Jung Lee ◽  
Hyoun-Ee Kim ◽  
Young-Hag Koh ◽  
Jun-Hyeog Jang
Keyword(s):  
Bone Regeneration ◽  
The Impact

scholarly journals The impact of thickness of resorbable membrane of human origin on the ossification of bone defects: A pathohistologic study

2012 ◽  
Vol 69 (12) ◽  
pp. 1076-1083 ◽  
Author(s):  
Marija Bubalo ◽  
Zoran Lazic ◽  
Smiljana Matic ◽  
Zoran Tatic ◽  
Radomir Milovic ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Bone Regeneration ◽  
Blood Clot ◽  
Bone Defects ◽  
Histopathological Analysis ◽  
Bony Defect ◽  
The Third ◽  
Barrier Membrane ◽  
Wide Range ◽  
The Impact

Background/Aim. A wide range of resorbable and nonresorbable membranes have been investigated over the last two decades. The barrier membrane protects the defect from ingrowth of soft tissue cells and allows bone progenitor cells to develop bone within a blood clot that is formed beneath the barrier membrane. The membranes are applied to reconstruct small bony defect prior to implantation, to cover dehiscences and fenestrations around dental implants. The aim of this study was to evaluate the influence of human resorbable demineralized membrane (RHDM) thickness on bone regeneration. Methods. The experiment, approved by Ethical Committee, was performed on 6 dogs and conducted into three phases. Bone defects were created in all the 6 dogs on the left side of the mandible, 8 weeks after extraction of second, third and fourth premolars. One defect was covered with RHDM 100 ? thick, one with RHDM 200 ? thick, and the third defect left empty (control defect). The histopathological analysis was done 2, 4 and 6 months after the surgery. In the third phase samples of bone tissue were taken and subjected to histopathological analysis. Results. In all the 6 dogs the defects treated with RHDM 200 ? thick showed higher level of bone regeneration in comparison with the defect treated with RHDM 100 ? thick and especially with empty defect. Conclusion. Our results demonstrated that the thicker membrane showed the least soft tissue ingrowths and promoted better bone formation at 6 months compared with a thinner one.

scholarly journals The Impact of Graphene Oxide on Bone Regeneration Therapies

10.5772/63333 ◽  
2016 ◽  
Author(s):  
Anca Hermenean ◽  
Sorina Dinescu ◽  
Mariana Ionita ◽  
Marieta Costache
Keyword(s):  
Graphene Oxide ◽  
Bone Regeneration ◽  
The Impact

scholarly journals The Effect of Laser Irradiation on Reparative Osteogenesis

2018 ◽  
Vol 1 (2) ◽  
pp. 10-19
Author(s):  
Yu. М. Iryanov ◽  
N. A. Kiryanov
Keyword(s):  
Laser Radiation ◽  
Radiation Exposure ◽  
Bone Regeneration ◽  
Laser Irradiation ◽  
Light And Electron Microscopy ◽  
Infrared Laser ◽  
Control Group ◽  
X Ray ◽  
Low Intensity ◽  
The Impact

Introduction: The use of non-medicinal facilities for correcting processes in various pathological conditions is one of the most urgent problems of modern medicine. Purpose of the Work: To study the effect of low-intensity infrared laser radiation on reparative bone formation and angiogenesis in bone regeneration which is formed in treatment of fractures under conditions of transosseous osteosynthesis. Material and Methods: A tibia fracture was modeled experimentally in rats in the control and experimental groups. Reposition and fixation of fragments were performed. The fracture zone in the experimental group animals was exposed to the impact of pulsed infrared laser irradiation of low intensity. Animals from the control group underwent the impact simulation. The operated bones were investigated using the methods of X-ray, light and electron microscopy, X-ray electron probe microanalysis. Results: It was established that laser radiation exposure sessions activated fibrillogenesis and angiogenesis, accelerated compacting of newly formed bone tissue and increased its maturity while primary fracture healing occurred. Prolonged capillary dilatation and endothelium-dependent vasodilation, intensive capillarogenesis were noted after sessions of laser therapy in bone regeneration. Endothelial outgrowth was formed in the lumen of the vessels forming capillary buds that propagate along the “mother” vessels (endovascular capillarogenesis). Conclusion: The data obtained revealed a possible mechanism of laser radiation exposure at the level of a whole organism and proved the effectiveness of its application in clinical practice at the early stages of patient rehabilitation under conditions of transosseous osteosynthesis.

scholarly journals MiR-144-5p, an exosomal miRNA from bone marrow-derived macrophage in type 2 diabetes, impairs bone fracture healing via targeting Smad1

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Dong Zhang ◽  
Yifan Wu ◽  
Zonghuan Li ◽  
Hairen Chen ◽  
Siyuan Huang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Fracture Healing ◽  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Fracture Repair ◽  
Osteogenic Potential ◽  
Patients With Diabetes ◽  
The Impact

Abstract Background Patients with diabetes have an increased risk of nonunion and delayed union of fractures. Macrophages have been shown as a key player in diabetic complications. However, it remains obscure how diabetic milieu affects macrophage-derived exosomes and its implications on osteogenic differentiation of BMSCs. In this study, we aim to define the impact of diabetic milieu on macrophage-derived exosomes, role of extracellular vesicles in intercellular communication with BMSCs, and subsequent effects on osteogenic differentiation and fracture repair. Results The osteogenic potential and the ability of fracture repair of exosomes derived from diabetic bone marrow-derived macrophages (dBMDM-exos) were revealed to be lower, as compared with non-diabetic bone marrow-derived macrophages (nBMDM-exos) in vitro and in vivo. Interestingly, miR-144-5p levels were sharply elevated in dBMDM-exos and it could be transferred into BMSCs to regulate bone regeneration by targeting Smad1. In addition, the adverse effects of dBMDM-exos on the osteogenic potential and the ability of fracture repair were reversed through the suppression of miR-144-5p inhibition in vitro and vivo. Conclusions The results demonstrated an important role of exosomal miR-144-5p in bone regeneration, offering insight into developing new strategy for the improvement of fracture healing in patients with diabetes mellitus. Graphic Abstract

scholarly journals Biomechanical Stability and Osteogenesis in a Tibial Bone Defect Treated by Autologous Ovine Cord Blood Cells—A Pilot Study

Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 295 ◽  
Author(s):  
Monika Herten ◽  
Christoph Zilkens ◽  
Fritz Thorey ◽  
Tjark Tassemeier ◽  
Sabine Lensing-Höhn ◽  
...  
Keyword(s):  
Cord Blood ◽  
Bone Regeneration ◽  
Bone Defect ◽  
Blood Cells ◽  
Test Group ◽  
Control Group ◽  
Biomechanical Stability ◽  
Tibial Bone ◽  
Cord Blood Cells ◽  
The Impact

The aim of this study was to elucidate the impact of autologous umbilical cord blood cells (USSC) on bone regeneration and biomechanical stability in an ovine tibial bone defect. Ovine USSC were harvested and characterized. After 12 months, full-size 2.0 cm mid-diaphyseal bone defects were created and stabilized by an external fixateur containing a rigidity measuring device. Defects were filled with (i) autologous USSC on hydroxyapatite (HA) scaffold (test group), (ii) HA scaffold without cells (HA group), or (iii) left empty (control group). Biomechanical measures, standardized X-rays, and systemic response controls were performed regularly. After six months, bone regeneration was evaluated histomorphometrically and labeled USSC were tracked. In all groups, the torsion distance decreased over time, and radiographies showed comparable bone regeneration. The area of newly formed bone was 82.5 ± 5.5% in the control compared to 59.2 ± 13.0% in the test and 48.6 ± 2.9% in the HA group. Labeled cells could be detected in lymph nodes, liver and pancreas without any signs of tumor formation. Although biomechanical stability was reached earliest in the test group with autologous USSC on HA scaffold, the density of newly formed bone was superior in the control group without any bovine HA.

The impact of proton pump inhibitors on bone regeneration and implant osseointegration

2019 ◽  
Vol 51 (3) ◽  
pp. 330-339 ◽  
Author(s):  
Alexandru Mester ◽  
Dragos Apostu ◽  
Lidia Ciobanu ◽  
Andra Piciu ◽  
Ondine Lucaciu ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Proton Pump Inhibitors ◽  
Proton Pump ◽  
The Impact

scholarly journals The Effect of Diclofenac Sodium-Loaded Poly(Lactide-co-Glycolide) Rods on Bone Formation and Inflammation: A Histological and Histomorphometric Study in the Femora of Rats

Micromachines ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 1098
Author(s):  
Karoline M. Reich ◽  
Petrus Viitanen ◽  
Ehsanul Hoque Apu ◽  
Stefan Tangl ◽  
Nureddin Ashammakhi
Keyword(s):  
Bone Regeneration ◽  
Diclofenac Sodium ◽  
Giant Cells ◽  
Host Tissue ◽  
Negative Effects ◽  
Implant Surface ◽  
Histomorphometric Study ◽  
Tissue Reactions ◽  
Local Release ◽  
The Impact

Implants made of poly(lactide-co-glycolide) (PLGA) are biodegradable and frequently provoke foreign body reactions (FBR) in the host tissue. In order to modulate the inflammatory response of the host tissue, PLGA implants can be loaded with anti-inflammatory drugs. The aim of this study was to analyze the impact of PLGA 80/20 rods loaded with the diclofenac sodium (DS) on local tissue reactions in the femur of rats. Special emphasis was put on bone regeneration and the presence of multinucleated giant cells (MGCs) associated with FBR. PLGA 80/20 alone and PLGA 80/20 combined with DS was extruded into rods. PLGA rods loaded with DS (PLGA+DS) were implanted into the femora of 18 rats. Eighteen control rats received unloaded PLGA rods. The follow-up period was of 3, 6 and 12 weeks. Each group comprised of six rats. Peri-implant tissue reactions were histologically and histomorphometrically evaluated. The implantation of PLGA and PLGA+DS8 rods induced the formation of a layer of newly formed bone islands parallel to the contour of the implants. PLGA+DS rods tended to reduce the presence of multi-nucleated giant cells (MGCs) at the implant surface. Although it is known that the systemic administration of DS is associated with compromised bone healing, the local release of DS via PLGA rods did not have negative effects on bone regeneration in the femora of rats throughout 12 weeks.

scholarly journals The impact of surface roughness and permeability in hydroxyapatite bone regeneration membranes

2015 ◽  
Vol 27 (8) ◽  
pp. 1047-1054 ◽  
Author(s):  
Jonas Anderud ◽  
Ryo Jimbo ◽  
Peter Abrahamsson ◽  
Erik Adolfsson ◽  
Johan Malmström ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Bone Regeneration ◽  
The Impact

scholarly journals The impact of the size of bone substitute granules on macrophage and osteoblast behaviors in vitro

2021 ◽  
Author(s):  
Masako Fujioka-Kobayashi ◽  
Hiroki Katagiri ◽  
Michihide Kono ◽  
Benoit Schaller ◽  
Tateyuki Iizuka ◽  
...  
Keyword(s):  
Cell Viability ◽  
Bone Regeneration ◽  
Bone Substitute ◽  
Size Group ◽  
Bovine Bone ◽  
Carbonate Apatite ◽  
Mrna Levels ◽  
Differentiation Potential ◽  
The Impact

Abstract Objective Bone substitute (BS) size might influence the clinical outcomes of guided bone regeneration (GBR) procedures. The aim of the present study was to investigate the influence of BS size on macrophage (Mφ) and osteoblast behaviors in vitro. Materials and methods Two different granule sizes (S and M/L) were assessed for four different commercial BSs: deproteinized bovine bone mineral (DBBM), biphasic calcium phosphate type 1 (BCP1), BCP type 2 (BCP2), and carbonate apatite (CO3Ap). The BSs were compared for their impacts on the cell viability and differentiation potential of THP-1-derived Mφs and human osteoblast-like Saos-2 cells. Results The smaller granules showed higher material volumes and surface areas than the larger granules. Significantly higher viability of Mφs and Saos-2 cells was observed with the DBBM_L-size granules than with the DBBM_S-size granules. Gene expression experiments in Mφs revealed few differences between the two sizes of each BS, although higher CD206 mRNA levels were observed in the BCP1_L group and the CO3Ap_M group than in the respective S-size groups on day 1. Only DBBM showed significantly higher mRNA levels of osteogenic markers, including Runx2 and osteocalcin, in Saos-2 cells in the S-size group than in the L-size group. Conclusions The S-size and L-size DBBM granules exhibited clear differences in cell outcomes: cells cultured on the S-size granules exhibited lower cell viability, higher osteopromotive ability, and no noticeable Mφ polarization changes. Clinical relevance A smaller granule size might be advantageous due to greater bone regeneration potential in the use of DBBM granules to treat defects.

scholarly journals MiRNA- Nanofiber, the Next Generation of Bioactive Scaffolds for Bone Regeneration: A Review

Micromachines ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1472
Author(s):  
Davood Kharaghani ◽  
Eben Bashir Kurniwan ◽  
Muhammad Qamar Khan ◽  
Yuji Yoshiko
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Bone Development ◽  
Bioactive Molecules ◽  
Fibrous Scaffold ◽  
Bioactive Scaffolds ◽  
Alternative Treatment Option ◽  
The Impact

Scaffold-based bone tissue engineering has been introduced as an alternative treatment option for bone grafting due to limitations in the allograft. Not only physical conditions but also biological conditions such as gene expression significantly impact bone regeneration. Scaffolds in composition with bioactive molecules such as miRNA mimics provide a platform to enhance migration, proliferation, and differentiation of osteoprogenitor cells for bone regeneration. Among scaffolds, fibrous structures showed significant advantages in promoting osteogenic differentiation and bone regeneration via delivering bioactive molecules over the past decade. Here, we reviewed the bone and bone fracture healing considerations for the impact of miRNAs on bone regeneration. We also examined the methods used to improve miRNA mimics uptake by cells, the fabrication of fibrous scaffolds, and the effective delivery of miRNA mimics using fibrous scaffold and their processes for bone development. Finally, we offer our view on the principal challenges of miRNA mimics delivery by nanofibers for bone tissue engineering.

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