Evaluation of scaffold materials for tooth tissue engineering

2010 ◽  
Vol 9999A ◽  
pp. NA-NA ◽  
Author(s):  
Takayuki Ohara ◽  
Toshimitsu Itaya ◽  
Kazutada Usami ◽  
Yusuke Ando ◽  
Hiroya Sakurai ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Scaffold Materials ◽  
Tooth Tissue

Bioengineered Teeth from Cultured Rat Tooth Bud Cells

2004 ◽  
Vol 83 (7) ◽  
pp. 523-528 ◽  
Author(s):  
M.T. Duailibi ◽  
S.E. Duailibi ◽  
C.S. Young ◽  
J.D. Bartlett ◽  
J.P. Vacanti ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Engineering Applications ◽  
Adult Rat ◽  
Dental Tissues ◽  
Biodegradable Scaffolds ◽  
Bioengineered Tooth ◽  
Tooth Tissue

The recent bioengineering of complex tooth structures from pig tooth bud tissues suggests the potential for the regeneration of mammalian dental tissues. We have improved tooth bioengineering methods by comparing the utility of cultured rat tooth bud cells obtained from three- to seven-day post-natal (dpn) rats for tooth-tissue-engineering applications. Cell-seeded biodegradable scaffolds were grown in the omenta of adult rat hosts for 12 wks, then harvested. Analyses of 12-week implant tissues demonstrated that dissociated 4-dpn rat tooth bud cells seeded for 1 hr onto PGA or PLGA scaffolds generated bioengineered tooth tissues most reliably. We conclude that tooth-tissue-engineering methods can be used to generate both pig and rat tooth tissues. Furthermore, our ability to bioengineer tooth structures from cultured tooth bud cells suggests that dental epithelial and mesenchymal stem cells can be maintained in vitro for at least 6 days.

scholarly journals Tissue Engineering Scaffold Materials to Repair Sports Cartilage Injury

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Mo Xing
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Cell Suspension ◽  
Cell Density ◽  
Porous Scaffold ◽  
Cartilage Damage ◽  
Tissue Engineering Scaffold ◽  
Cartilage Injury ◽  
Culture Bottle ◽  
Scaffold Materials

With the continued development of sports in China, sports sometimes cause cartilage damage. The purpose of this research is to study the tissue engineering scaffold material for sports cartilage damage repair. In this study, mesenchymal rat bone marrow stem cells (to put it simply, stem cells are a type of cell with unlimited or immortal self-renewal capacity, capable of producing at least one type of highly differentiated progeny cells) were obtained by the total bone wash method. The cells were inoculated into the cell culture bottle. When the primary cultured cells proliferated to about 80% of the culture bottle area, the cells were digested with trypsin to open the cell link, then the medium containing 10% serum was added to terminate the cell digestion, and then the passage expansion was carried out according to the cell density. PLGA/NHA and PLGA were heated to 65°C under ultrasonic vibration until uniform PLGA/NHA and PLGA solutions were obtained. Then, the samples were added to the tube mold and then heated and cooled to obtain the composite porous scaffold of mesenchymal stem cells. 10 μl MSCs cell suspension was extracted with a microinjector, and the needle was injected from the inside of the scaffold, and the cell suspension was added outside the scaffold to ensure that there were composite cells inside and outside the scaffold. The subcutaneous tissue of the skin was cut along the medial side of the knee joint and the capsule of the switch segment was cut. The scaffold materials were filled into the osteochondral defect to observe the cartilage healing. The mechanical strength of 0.5% PLGA-MSCs composite porous scaffold was increased to 1.1 MPa, and the cell density was high. The repair of cartilage in rats was the best. The results showed that the porous scaffolds designed in this study have good compatibility and are beneficial to repair sports cartilage injury.

Preparation of Electrospun Poly(ε-caprolactone)/Poly(trimethylene carbonate) Blend Scaffold for In Situ Vascular Tissue Engineering

2012 ◽  
Vol 629 ◽  
pp. 60-63
Author(s):  
Tao Jiang ◽  
Guo Quan Zhang ◽  
Hui Li ◽  
Ji Na Xun
Keyword(s):  
Tissue Engineering ◽  
Vascular Graft ◽  
Vascular Tissue ◽  
Electrospinning Process ◽  
Vascular Tissue Engineering ◽  
Trimethylene Carbonate ◽  
Scaffold Materials ◽  
Degradation Time

In the active field of vascular graft research, in situ vascular tissue engineering is a novel concept. This approach aims to use biodegradable synthetic materials. After implantation, the synthetic material progressively degrades and should be replaced by autologous cells. Poly (ε-caprolactone) (PCL) is often used for vascular graft because of its good mechanical strength and its biocompatibility. It is easily processed into micro and nano-fibers by electrospinning to form a porous, cell-friendly scaffold. However, the degradation time of polycaprolactone is too long to match the tissue regeneration time. In this study, poly (ε-caprolactone) /poly (trimethylene carbonate) (PTMC) blend scaffold materials have been prepared for biodegradable vascular graft using an electrospinning process. Because the degradation time of PTMC is shorter than PCL in vivo. The morphological characters of PCL/PTMC blend scaffold materials were investigated by scanning electron microscope (SEM). The molecular components and some physical characteristics of the blend scaffold materials were tested by FT-IR and DSC analysis.

Application of Different Biomaterials in Achilles Tendon Repair for Exercise Injury

2014 ◽  
Vol 886 ◽  
pp. 329-332
Author(s):  
Na Zhao ◽  
Wen Chen ◽  
Tao Yan
Keyword(s):  
Tissue Engineering ◽  
Achilles Tendon ◽  
Tendon Repair ◽  
Functional Differentiation ◽  
Tendon Injury ◽  
Self Healing ◽  
Advantages And Disadvantages ◽  
Tendon Tissue Engineering ◽  
Tendon Transplantation ◽  
Scaffold Materials

The rupture of Achilles tendon is hard to self-healing and repair and it is easily left pain and dysfunction. For a long time, the treatment of Achilles tendon defect by many scholars conducted a lot of research, from the tendon autograft, allograft tendon transplantation to the artificial tendon transplantation, tissue engineering tendon transplantation. Practice has proved that these methods have their own advantages and disadvantages. Although the research and application of scaffold materials for tendon tissue engineering has achieved some success, but the application materials or the presence of biocompatibility, degradation problems or have poor mechanical properties, machining molding defects, there is still a big gap between the ideal scaffold materials. This study evaluated the different biological materials in the repair of Achilles tendon injury in effect, provide a theoretical reference for the key to construct tissue engineered tendon is to find appropriate scaffold materials for tendon cell adhesion, growth and functional differentiation.

Cytocompatibility of Poly(L-lactide-co-glycolide) Porous Scaffold Materials for Tissue Engineering

2008 ◽  
Vol 57 (11) ◽  
pp. 1026-1035 ◽  
Author(s):  
Zhihua Zhou ◽  
Xiaoping Liu ◽  
Lihua Liu ◽  
Qingquan Liu ◽  
Qingfeng Yi
Keyword(s):  
Tissue Engineering ◽  
Porous Scaffold ◽  
Scaffold Materials

Poly(1,3-Butylene Fumerate) and Poly(1,3-Butylene Fumerate)-co-(1,3-Butylene Maleate) as Electrospun Scaffold Materials

2009 ◽  
Vol 1239 ◽  
Author(s):  
Kirsten Nicole Cicotte ◽  
Shawn M. Dirk ◽  
Elizabeth Hedberg-Dirk
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Ring Opening ◽  
Room Temperature ◽  
Electrospinning Process ◽  
Crosslinking Reaction ◽  
Electrospun Scaffold ◽  
Fiber Mats ◽  
Nano Fiber ◽  
Scaffold Materials

AbstractPoly(butylene fumerate) (PBF) and poly(butylene fumerate)-co-(butylene maleate) (PBFcBM) have been synthesized from the ring opening and condensation reactions of maleic anhydride (MA) and 1,3-butanediol (BD). PBFcBM synthesized in this way contains greater than 85% maleate groups. Both PBF and PBFcBM have a glass transition temperature (Tg) below room temperature and therefore cannot be electrospun using the conventional electrospinning process as a non-porous film results. To facilitate production of nonwoven micro- and nano-fiber mats, a UV-source (λ=356 nm) was used in combination with a photoinitator loaded polymer solution to initiate the crosslinking reaction of the fumerate and maleate functional groups as the fibers were produced. The resulting non-woven fiber mats are potentially suitable scaffolds for tissue engineering and drug delivery application.

scholarly journals Current Advance and Future Prospects of Tissue Engineering Approach to Dentin/Pulp Regenerative Therapy

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Ting Gong ◽  
Boon Chin Heng ◽  
Edward Chin Man Lo ◽  
Chengfei Zhang
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Animal Studies ◽  
Banking System ◽  
Engineering Technology ◽  
Potential Health ◽  
Regenerative Endodontics ◽  
Immunological Rejection ◽  
Canal Systems ◽  
Scaffold Materials

Recent advances in biomaterial science and tissue engineering technology have greatly spurred the development of regenerative endodontics. This has led to a paradigm shift in endodontic treatment from simply filling the root canal systems with biologically inert materials to restoring the infected dental pulp with functional replacement tissues. Currently, cell transplantation has gained increasing attention as a scientifically valid method for dentin-pulp complex regeneration. This multidisciplinary approach which involves the interplay of three key elements of tissue engineering—stem cells, scaffolds, and signaling molecules—has produced an impressive number of favorable outcomes in preclinical animal studies. Nevertheless, many practical hurdles need to be overcome prior to its application in clinical settings. Apart from the potential health risks of immunological rejection and pathogenic transmission, the lack of a well-established banking system for the isolation and storage of dental-derived stem cells is the most pressing issue that awaits resolution and the properties of supportive scaffold materials vary across different studies and remain inconsistent. This review critically examines the classic triad of tissue engineering utilized in current regenerative endodontics and summarizes the possible techniques developed for dentin/pulp regeneration.

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