Effects of fluid flow and calcium phosphate coating on human bone marrow stromal cells cultured in a defined 2D model system

2008 ◽  
Vol 86A (2) ◽  
pp. 411-419 ◽  
Author(s):  
S. Scaglione ◽  
D. Wendt ◽  
S. Miggino ◽  
A. Papadimitropoulos ◽  
M. Fato ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Fluid Flow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Model System ◽  
Marrow Stromal Cells ◽  
Calcium Phosphate Coating ◽  
Phosphate Coating ◽  
2D Model ◽  
Cells Cultured

Adipocyte Differentiation of SOD2−/− Mouse Bone Marrow Stromal Cells Is Associated with Decreased Antioxidant Reserves and Is Reversed by the Antioxidant WR2721 (Amifostine).

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 2342-2342
Author(s):  
Michael W. Epperly ◽  
Stanislav Lechpammer ◽  
Suhua Nie ◽  
Julie Glowacki ◽  
Joel S. Greenberger
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Adipocyte Differentiation ◽  
Day Treatment ◽  
Marrow Stromal Cells ◽  
Differentiation Potential ◽  
Gpx Activity ◽  
Oil Red O ◽  
Cells Cultured

Abstract Cell lines from homozygous deletion recombinant negative manganese superoxide dismutase (SOD2 −/−) mice have intrinsic ionizing radiation sensitivity that is reversed by expression of the transgene for human SOD2 (Radiation Research154:365, 2000). This study tests whether redox status influences adipocyte differentiation potential of bone marrow stromal cells by comparing the differentiation potential of SOD2−/− and SOD2 +/+ bone marrow stromal cell lines. Cells were cultured in basal medium (Dulbecco’s Modified Eagle’s Medium, 1% fetal bovine serum (FBS), 100 U/ml penicillin and 100 μg/mL streptomycin +/− adipocytogenic supplements (10 μg/ml insulin, 1 μM dexamethasone, 100 mM indomethacin)). Adipocytogenesis was assessed by spectrophotometric content of oil red-O dye and by RT-PCR for peroxisome proliferator-activated receptor-gamma (PPARγ) and lipoprotein lipase (LPL). Glutathione peroxidase (GPX) activity or glutathione (GSH) levels were measured by a Glutathione Peroxidase, Cellular Assay Kit or Glutathione Assay Kit, respectively, by Calbiochem, Inc. SOD2+/+ cells developed adipocytes only when treated with adipocytogenic supplements and expressed PPARγ and LPL at day 5. SOD2−/− cells cultured in basal conditions demonstrated constitutive adipocytogenesis. The level of GPX activity in SOD2−/− cells (43.2 ± 3.5 mU/ml) was 52.7% (p<0.001) of that in SOD2+/+ cells (82.0 ± 2.3). The level of GSH in SOD2−/− cells (173 ± 2 μM) was 78.6% (p=0.0089) of that in SOD2+/+ cells (220 ± 4 μM). Three day treatment of SOD2−/−with 4 mM WR2721 resulted in 22% reduction in oil red-O content. In addition, in SOD2−/− cells cultured in adipocytogenic medium, 3-day treatment in 4 mM WR2721 resulted in 46% reduction in oil red-O content and undetectable expression of PPARγ and LPL. In conclusion, WR2721 limits constitutive adipocytogenesis and inhibits induced adipocytogenesis in SOD2−/− cells. These data provide evidence for the involvement of the cellular redox pathway in adipocyte differentiation of bone marrow stromal cells.

Osteoblast differentiation of bone marrow stromal cells cultured on silica gel and sol–gel-derived titania

Biomaterials ◽  
2002 ◽  
Vol 23 (14) ◽  
pp. 3041-3051 ◽  
Author(s):  
S.C Dieudonné ◽  
J van den Dolder ◽  
J.E de Ruijter ◽  
H Paldan ◽  
T Peltola ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Silica Gel ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Osteoblast Differentiation ◽  
Sol Gel ◽  
Marrow Stromal Cells ◽  
Cells Cultured

Osteogenic differentiation of rat bone marrow stromal cells cultured on Arg–Gly–Asp modified hydrogels without dexamethasone and β-glycerol phosphate

Biomaterials ◽  
2005 ◽  
Vol 26 (17) ◽  
pp. 3645-3654 ◽  
Author(s):  
Heungsoo Shin ◽  
Johnna S. Temenoff ◽  
Gregory C. Bowden ◽  
Kyriacos Zygourakis ◽  
Mary C. Farach-Carson ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Glycerol Phosphate ◽  
Cells Cultured ◽  
Rat Bone
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