Three-dimensional culture of porcine fetal liver cells for a bioartificial liver

2006 ◽  
Vol 77A (1) ◽  
pp. 90-96 ◽  
Author(s):  
Tomo Ehashi ◽  
Norio Ohshima ◽  
Hirotoshi Miyoshi
Keyword(s):  
Fetal Liver ◽  
Three Dimensional ◽  
Liver Cells ◽  
Bioartificial Liver ◽  
Fetal Liver Cells ◽  
Three Dimensional Culture

Expansion of mouse hematopoietic stem/progenitor cells in three-dimensional cocultures on growth-suppressed stromal cell layer

2019 ◽  
Vol 42 (7) ◽  
pp. 374-379 ◽  
Author(s):  
Hirotoshi Miyoshi ◽  
Chiaki Sato ◽  
Yuichiro Shimizu ◽  
Misa Morita
Keyword(s):  
Progenitor Cells ◽  
Mitomycin C ◽  
Stromal Cells ◽  
Fetal Liver ◽  
Three Dimensional ◽  
Hematopoietic Cells ◽  
Liver Cells ◽  
Hematopoietic Progenitor ◽  
Hematopoietic Stem ◽  
Fetal Liver Cells

With the aim of establishing an effective method to expand hematopoietic stem/progenitor cells for application in hematopoietic stem cell transplantation, we performed ex vivo expansion of hematopoietic stem/progenitor cells derived from mouse fetal liver cells in three-dimensional cocultures with stromal cells. In these cocultures, stromal cells were first cultured within three-dimensional scaffolds to form stromal layers and then fetal liver cells containing hematopoietic cells were seeded on these scaffolds to expand the hematopoietic cells over the 2 weeks of coculture in a serum-containing medium without the addition of cytokines. Prior to coculture, stromal cell growth was suppressed by treatment with the DNA synthesis inhibitor mitomycin C, and its effect on hematopoietic stem/progenitor cell expansion was compared with that in control cocultures in which fetal liver cells were cocultured with three-dimensional freeze-thawed stromal cells. After coculture with mitomycin C-treated stromal cells, we achieved a several-fold expansion of the primitive hematopoietic cells (c-kit+hematopoietic progenitor cells >7.8-fold, and CD34+hematopoietic stem/progenitor cells >3.5-fold). Compared with control cocultures, expansion of hematopoietic stem/progenitor cells tended to be lower, although that of hematopoietic progenitor cells was comparable. Thus, our results suggest that three-dimensional freeze-thawed stromal cells have higher potential to expand hematopoietic stem/progenitor cells compared with mitomycin C-treated stromal cells.

Three-Dimensional Perfusion Bioreactor Culture Supports Differentiation of Human Fetal Liver Cells

2010 ◽  
Vol 16 (6) ◽  
pp. 2007-2016 ◽  
Author(s):  
Eva Schmelzer ◽  
Fabio Triolo ◽  
Morris E. Turner ◽  
Robert L. Thompson ◽  
Katrin Zeilinger ◽  
...  
Keyword(s):  
Fetal Liver ◽  
Three Dimensional ◽  
Liver Cells ◽  
Perfusion Bioreactor ◽  
Bioreactor Culture ◽  
Human Fetal Liver ◽  
Fetal Liver Cells ◽  
Human Fetal Liver Cells

Long-term cultures of human fetal liver cells: a three-dimensional experimental model for monitoring liver tissue development

1998 ◽  
Vol 28 (3) ◽  
pp. 480-490 ◽  
Author(s):  
Massimo Sargiacomo ◽  
Paolo Onori ◽  
Elena Bravo ◽  
Antonio Franchitto ◽  
Alfredo Cantafora ◽  
...  
Keyword(s):  
Experimental Model ◽  
Liver Tissue ◽  
Fetal Liver ◽  
Three Dimensional ◽  
Liver Cells ◽  
Tissue Development ◽  
Human Fetal Liver ◽  
Fetal Liver Cells ◽  
Human Fetal Liver Cells

scholarly journals Mouse Fetal Liver Cells in Artificial Capillary Beds in Three-Dimensional Four-Compartment Bioreactors

2005 ◽  
Vol 167 (5) ◽  
pp. 1279-1292 ◽  
Author(s):  
Satdarshan P.S. Monga ◽  
Mariah S. Hout ◽  
Matt J. Baun ◽  
Amanda Micsenyi ◽  
Peggy Muller ◽  
...  
Keyword(s):  
Fetal Liver ◽  
Three Dimensional ◽  
Liver Cells ◽  
Fetal Liver Cells ◽  
Artificial Capillary

LONG-TERM CULTURE OF FETAL LIVER CELLS USING THREE-DIMENSIONAL POROUS POLYMER

ASAIO Journal ◽  
1999 ◽  
Vol 45 (2) ◽  
pp. 129
Author(s):  
H Miyoshi ◽  
T Ehashi ◽  
H Ema ◽  
H C Hsu ◽  
H Nakauchi ◽  
...  
Keyword(s):  
Fetal Liver ◽  
Three Dimensional ◽  
Liver Cells ◽  
Porous Polymer ◽  
Long Term Culture ◽  
Fetal Liver Cells
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