Meniscal regeneration using tissue engineering with a scaffold derived from a rat meniscus and mesenchymal stromal cells derived from rat bone marrow

2005 ◽  
Vol 75A (1) ◽  
pp. 23-30 ◽  
Author(s):  
Takuma Yamasaki ◽  
Masataka Deie ◽  
Rikuo Shinomiya ◽  
Yasunori Izuta ◽  
Yuji Yasunaga ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Rat Bone

Transplantation of Meniscus Regenerated by Tissue Engineering With a Scaffold Derived From a Rat Meniscus and Mesenchymal Stromal Cells Derived From Rat Bone Marrow

2008 ◽  
Vol 32 (7) ◽  
pp. 519-524 ◽  
Author(s):  
Takuma Yamasaki ◽  
Masataka Deie ◽  
Rikuo Shinomiya ◽  
Yuji Yasunaga ◽  
Shinobu Yanada ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Rat Bone

CHARACTERIZATION AND EVALUATION OF TITANIUM SUBSTRATES COATED WITH GELATIN/HYDROXYAPATITE COMPOSITE FOR CULTURING RAT BONE MARROW DERIVED MESENCHYMAL STROMAL CELLS

2012 ◽  
Vol 24 (03) ◽  
pp. 197-206 ◽  
Author(s):  
Yuanyuan Chen ◽  
Hongbo Zhao ◽  
Rui Wang ◽  
Bo Lan ◽  
Linhong Deng
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Bone Tissue ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Surface Structures ◽  
Porous Surface ◽  
Force Microscopy ◽  
Gelatin Concentration ◽  
Rat Bone

Titanium (Ti) is widely used for making tissue engineering implants, due to its good corrosion resistance, biocompatibility and mechanical properties. However, bare Ti does not integrate well with natural bone tissue and releases ions and particles that are harmful to the extracellular matrix. To overcome these problems, the Ti substrate could be coated with various biocompatible materials including a composite of gelatin and hydroxyapatite (HA). However, few have characterized and evaluated the coating of gelatin/HA on Ti substrate and its effect on bone related cells. In this study, samples of Ti substrate coated with gelatin/HA composite were fabricated with gelatin concentration ranging from 0 to 200 mg/L. The porous surface structure of gelatin/HA composite formed on the Ti substrate was then examined and characterized for its composition and topography by X-ray diffraction (XRD), scanning electron microscopy (SEM), atomic force microscopy (AFM), and image processing and analysis software (ImageJ), respectively. Subsequently, rat bone marrow-derived mesenchymal stromal cells (BMMSC) were cultured on the surface of gelatin/HA composite on Ti substrate, and evaluated for cell morphology, proliferation, and osteo-differentiation using SEM, MTT assay, and alkaline phosphatase (ALP) assay, respectively. It is shown that gelatin enhanced binding of HA onto Ti substrate, and the topography of the porous surface structures was influenced by gelatin concentration only for the large pore sizes. Furthermore, the results indicate that the porous surface structures of gelatin/HA on Ti substrate promoted proliferation and osteo–differentiation as compared to the naked pure Ti substrate, particularly on that with concentration of gelatin at 100 mg/L. These findings, taken together, suggest that Ti substrate can be coated with different porous surface structures of gelatin/HA composite with gelatin solution of different concentrations. Such coated Ti substrates can promote cytocompitibility and osteo-differentiation of BMMSC, and thus may be of potential in development of implants and devices for bone tissue engineering.

scholarly journals Erratum to “Preconditioning of Rat Bone Marrow-Derived Mesenchymal Stromal Cells with Toll-Like Receptor Agonists”

2020 ◽  
Vol 2020 ◽  
pp. 1-2
Author(s):  
Fabiana Evaristo-Mendonça ◽  
Gabriela Sardella-Silva ◽  
Tais Hanae Kasai-Brunswick ◽  
Raquel Maria Pereira Campos ◽  
Pablo Domizi ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Toll Like Receptor ◽  
Receptor Agonists ◽  
Rat Bone

Study of growth parameters and potentialities of differentiation of multipotent mesenchymal stromal cells from rat bone marrow in vitro

2006 ◽  
Vol 141 (4) ◽  
pp. 530-535 ◽  
Author(s):  
N. S. Sergeeva ◽  
I. K. Sviridova ◽  
V. A. Kirsanova ◽  
S. A. Akhmedova ◽  
N. V. Marshutina ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Growth Parameters ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Rat Bone

Growth on poly(l-lactic acid) porous scaffold preserves CD73 and CD90 immunophenotype markers of rat bone marrow mesenchymal stromal cells

2014 ◽  
Vol 25 (10) ◽  
pp. 2421-2436 ◽  
Author(s):  
Alessandra Zamparelli ◽  
Nicoletta Zini ◽  
Luca Cattini ◽  
Giulia Spaletta ◽  
Davide Dallatana ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Lactic Acid ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Porous Scaffold ◽  
Rat Bone

Inhibition of myostatin promotes myogenic differentiation of rat bone marrow-derived mesenchymal stromal cells

Cytotherapy ◽  
2009 ◽  
pp. 1-14
Author(s):  
Jia Geng ◽  
Funing Peng ◽  
Fu Xiong ◽  
Yanchang Shang ◽  
Cuiping Zhao ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Myogenic Differentiation ◽  
Rat Bone

Inhibition of myostatin promotes myogenic differentiation of rat bone marrow-derived mesenchymal stromal cells

Cytotherapy ◽  
2009 ◽  
Vol 11 (7) ◽  
pp. 849-863 ◽  
Author(s):  
Jia Geng ◽  
Funing Peng ◽  
Fu Xiong ◽  
Yanchang Shang ◽  
Cuiping Zhao ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Myogenic Differentiation ◽  
Rat Bone
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