Self-Assembled Extracellular Macromolecular Matrices and Their Different Osteogenic Potential with Preosteoblasts and Rat Bone Marrow Mesenchymal Stromal Cells

2012 ◽  
Vol 13 (9) ◽  
pp. 2811-2820 ◽  
Author(s):  
Soon Eon Bae ◽  
Suk Ho Bhang ◽  
Byung-Soo Kim ◽  
Kwideok Park
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Osteogenic Potential ◽  
Self Assembled ◽  
Rat Bone

scholarly journals Erratum to “Preconditioning of Rat Bone Marrow-Derived Mesenchymal Stromal Cells with Toll-Like Receptor Agonists”

2020 ◽  
Vol 2020 ◽  
pp. 1-2
Author(s):  
Fabiana Evaristo-Mendonça ◽  
Gabriela Sardella-Silva ◽  
Tais Hanae Kasai-Brunswick ◽  
Raquel Maria Pereira Campos ◽  
Pablo Domizi ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Toll Like Receptor ◽  
Receptor Agonists ◽  
Rat Bone

Study of growth parameters and potentialities of differentiation of multipotent mesenchymal stromal cells from rat bone marrow in vitro

2006 ◽  
Vol 141 (4) ◽  
pp. 530-535 ◽  
Author(s):  
N. S. Sergeeva ◽  
I. K. Sviridova ◽  
V. A. Kirsanova ◽  
S. A. Akhmedova ◽  
N. V. Marshutina ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Growth Parameters ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Rat Bone

Growth on poly(l-lactic acid) porous scaffold preserves CD73 and CD90 immunophenotype markers of rat bone marrow mesenchymal stromal cells

2014 ◽  
Vol 25 (10) ◽  
pp. 2421-2436 ◽  
Author(s):  
Alessandra Zamparelli ◽  
Nicoletta Zini ◽  
Luca Cattini ◽  
Giulia Spaletta ◽  
Davide Dallatana ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Lactic Acid ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Porous Scaffold ◽  
Rat Bone

scholarly journals Endogenous TGF-beta1 Mediated Osteogenic Impairment of Medullar Mesenchymal Stromal Cells in Primary Myelofibrosis

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 1873-1873
Author(s):  
Christophe Martinaud ◽  
Christophe Desterke ◽  
Johanna Konopacki ◽  
Lisa Pieri ◽  
Rachel Golub ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Conflicts Of Interest ◽  
Myeloproliferative Neoplasm ◽  
Hematopoietic Cells ◽  
Primary Myelofibrosis ◽  
Osteogenic Potential ◽  
Healthy Donors

Abstract Primary myelofibrosis (PMF) is myeloproliferative neoplasm characterized by clonal myeloproliferation, dysmegakaryopoiesis, extramedullary hematopoiesis associated with myelofibrosis and altered stroma in bone marrow and spleen. Mesenchymal stromal cells (MSCs) are reported to play a pivotal role in fibrosis and stromal changes are considered as a reactive counterpart of the cytokine production by clonal hematopoietic cells. The present study shows that MSCs from patients demonstrate functional abnormalities that are unexpectedly maintained ex-vivo, in culture. Material and Methods: we studied MSCs and bone marrow sections from PMF patients (n=12) as compared to healthy donors (HDs) (n=6). We tested their proliferation, immunophenotype, hematopoiesis supporting capacities, differentiation abilities, in-vivo osteogenic assays, and performed secretome and transcriptome analysis. Results: We found that PMF-MSCs exhibit similar proliferative capacity and long-term hematopoiesis supporting abilities as compare to healthy donors. They overproduce interleukin 6, VEGF, RANTES, PDGF, BMP-2 and surprisingly TGF-beta1. MSCs from fibrotic PMF patients express high levels of glycosaminoglycans. Adipocytes and chondrocytes differentiation abilities were not different as compared to HDs but PMF-MSCs exhibit an increased in vitro potential. Implementation on scaffold in nude mice confirmed, in vivo, this increased osteogenic potential. We then looked into gene expression and discovered that PMF-MSCs show an original transcriptome signature related to osteogenic lineage and TGF-beta1. Indeed, osteogenic genes such as Runx2, Dlx5, Twist1, Noggin, Sclerostin, GDF5 and Serpine1 are deregulated and suggest a potential osteoprogenitor priming of PMF-MSCs. These molecular results also advocated for a TGF-beta1 impregnation that prompted us to study its impact on PMF-MSCs osteogenic differentiation. First, we then showed that Smad2 is intrinsically over-activated in PMF-MSC and that stimulation by TGF-beta1 is associated with an increase phospho-Smad2 level and an enhancement of bone master gene regulator Runx2 expression. Then, we inhibited TGF-beta1 pathway by by SB-431542 and evidenced a specific behavior of osteogenic MSCs differentiation in patients, suggesting involvement of TGF-beta1 in osteogenic impairment. Conclusion: Altogether, our results identify a signature of PMF-MSCs and suggest that they participate in PMF osteogenic dysregulation independently from in vivo local stimulation by clonal hematopoietic cells Disclosures No relevant conflicts of interest to declare.

Inhibition of myostatin promotes myogenic differentiation of rat bone marrow-derived mesenchymal stromal cells

Cytotherapy ◽  
2009 ◽  
pp. 1-14
Author(s):  
Jia Geng ◽  
Funing Peng ◽  
Fu Xiong ◽  
Yanchang Shang ◽  
Cuiping Zhao ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Myogenic Differentiation ◽  
Rat Bone

Inhibition of myostatin promotes myogenic differentiation of rat bone marrow-derived mesenchymal stromal cells

Cytotherapy ◽  
2009 ◽  
Vol 11 (7) ◽  
pp. 849-863 ◽  
Author(s):  
Jia Geng ◽  
Funing Peng ◽  
Fu Xiong ◽  
Yanchang Shang ◽  
Cuiping Zhao ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Myogenic Differentiation ◽  
Rat Bone

Transplantation of Meniscus Regenerated by Tissue Engineering With a Scaffold Derived From a Rat Meniscus and Mesenchymal Stromal Cells Derived From Rat Bone Marrow

2008 ◽  
Vol 32 (7) ◽  
pp. 519-524 ◽  
Author(s):  
Takuma Yamasaki ◽  
Masataka Deie ◽  
Rikuo Shinomiya ◽  
Yuji Yasunaga ◽  
Shinobu Yanada ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Rat Bone

In Vivo Osteogenic Potential of Mesenchymal Stromal Cells Derived from Patient''s Bone Marrow on Hydroxyapatite Ceramics

2007 ◽  
pp. 1157-1160
Author(s):  
Asako Matsushima ◽  
Noriko Kotobuki ◽  
Hiroko Machida ◽  
Toru Morishita ◽  
Yoshinori Takakura ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Osteogenic Potential ◽  
Hydroxyapatite Ceramics
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