In vivo validation of a cell culture test for biocompatibility testing of urinary catheters

1984 ◽  
Vol 18 (9) ◽  
pp. 1125-1135 ◽  
Author(s):  
D. T. Graham ◽  
G. E. Mark ◽  
A. R. Pomeroy ◽  
E. B. Macarthur
Keyword(s):  
Cell Culture ◽  
Urinary Catheters ◽  
Culture Test ◽  
A Cell ◽  
Biocompatibility Testing ◽  
Cell Culture Test ◽  
In Vivo Validation

The activity of environmental samples in a cell culture test for asbestos toxicity

1980 ◽  
Vol 22 (2) ◽  
pp. 315-321 ◽  
Author(s):  
Betti Reiss ◽  
James R. Millette ◽  
Gary M. Williams
Keyword(s):  
Cell Culture ◽  
Environmental Samples ◽  
Culture Test ◽  
A Cell ◽  
Cell Culture Test

scholarly journals In Vitro Corrosion and Cell Response of Hydroxyapatite Coated Mg Matrix in Situ Composites for Biodegradable Material Applications

Materials ◽  
2019 ◽  
Vol 12 (21) ◽  
pp. 3474 ◽  
Author(s):  
Cao ◽  
Le ◽  
Pham ◽  
Nguyen ◽  
Hiromoto ◽  
...  
Keyword(s):  
Cell Culture ◽  
Corrosion Resistance ◽  
Solution Treatment ◽  
Potential Candidate ◽  
Test Results ◽  
Biodegradable Implant ◽  
Culture Test ◽  
Sintering Time ◽  
Cell Culture Test

In this study, hydroxyapatite (HAp) coated Mg matrix composites were fabricated for biodegradable implant applications. Spark plasma sintering was employed to fabricate the Mg-10 wt% ZnO composite substrates. HAp was coated on the surface of the sintered composites and pure Mg by a chemical solution treatment. SEM and optical micrographs of coated samples showed that HAp grew homogeneously and formed a layer on the entire surface of both pure sintered Mg and Mg composites. The immersion and polarization test results demonstrated that the HAp coating significantly improved the corrosion resistance of the sintered composites. While the HAp coating layer is not effective in the improvement of the pure Mg substrate, cell culture test results revealed that the HAp coating improved cell adhesion and proliferation on the composites effectively through 72 h, while no cell could survive on the uncoated composites after 72 h. In addition, the corrosion tests and cell culture test results indicated that the composite with longer sintering time has better corrosion resistance and cell viability than those of the composite with shorter sintering time. The findings suggested that the HAp-coated Mg-10 wt% ZnO-2.5 h + 10 min composite is a high-potential candidate for biodegradable implant applications.

scholarly journals Human Astrovirus MLB Replication In Vitro: Persistence in Extraintestinal Cell Lines

2019 ◽  
Vol 93 (13) ◽  
Author(s):  
Diem-Lan Vu ◽  
Albert Bosch ◽  
Rosa M. Pintó ◽  
Enric Ribes ◽  
Susana Guix
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Cell Lines ◽  
Culture System ◽  
Unknown Etiology ◽  
Cell Culture System ◽  
Persistent Infections ◽  
A Cell ◽  
Replicative Cycle

ABSTRACT MLB astroviruses were identified 10 years ago in feces from children with gastroenteritis of unknown etiology and have been unexpectedly detected in severe cases of meningitis/encephalitis, febrile illness of unknown etiology, and respiratory syndromes. The aim of this study was to establish a cell culture system supporting MLB astrovirus replication. We used two clinical strains to infect several cell lines, an MLB1 strain from a gastroenteritis case, and an MLB2 strain associated with a neurologic infection. Efforts to propagate the viruses in the Caco-2 cell line were unsuccessful. In contrast, we identified two human nonintestinal cell lines, Huh-7 and A549, permissive for both genotypes. After serial passages in the Huh-7.5 cell line, the adapted strains were able to establish persistent infections in the Huh-7.5, Huh-7AI, and A549 cell lines, with high viral loads (up to 10 log10 genome copies/ml) detected by quantitative reverse transcription-PCR (RT-qPCR) in the culture supernatant. Immunofluorescence assays demonstrated infection in about 10% of cells in persistently infected cultures. Electron microscopy revealed particles of 32 to 33 nm in diameter after negative staining of cell supernatants and capsid arrays in ultrathin sections with a particularly high production in Huh-7.5 cells. Interferon (IFN) expression by infected cells and effect of exogenous IFN varied depending on the type of infection and the cell line. The availability of a cell culture system to propagate MLB astroviruses represents a key step to better understand their replicative cycle, as well as a source of viruses to conduct a wide variety of basic virologic studies. IMPORTANCE MLB astroviruses are emerging viruses infecting humans. More studies are required to determine their exact epidemiology, but several reports have already identified them as the cause of unexpected clinical diseases, including severe neurologic diseases. Our study provides the first description of a cell culture system for the propagation of MLB astroviruses, enabling the study of their replicative cycle. Moreover, we demonstrated the unknown capacity of MLB astrovirus to establish persistent infections in cell culture. Whether these persistent infections are also established in vivo remains unknown, but the clinical consequences would be of high interest if persistence was confirmed in vivo. Finally, our analysis of IFN expression provides some trails to understand the mechanism by which MLB astroviruses can cause persistent infections in the assayed cultures.

Workshop on Cell-Culture Test Methods

2009 ◽  
pp. 127-127-27
Author(s):  
SA Brown ◽  
TD Nickel ◽  
TW Walker
Keyword(s):  
Cell Culture ◽  
Test Methods ◽  
Culture Test ◽  
Cell Culture Test

Detection of teratogens in theDrosophila embryonic cell culture test: Assay of 100 chemicals

Teratology ◽  
1983 ◽  
Vol 28 (1) ◽  
pp. 109-122 ◽  
Author(s):  
Nicole Bournias-Vardiabasis ◽  
Raymond L. Teplitz ◽  
Gerald F. Chernoff ◽  
Robert L. Seecof
Keyword(s):  
Cell Culture ◽  
Embryonic Cell ◽  
Culture Test ◽  
Cell Culture Test

Cell culture test of TCP/CPLA composite

1999 ◽  
Vol 48 (2) ◽  
pp. 108-110 ◽  
Author(s):  
Masanori Kikuchi ◽  
Junzo Tanaka ◽  
Yoshihisa Koyama ◽  
Kazuo Takakuda
Keyword(s):  
Cell Culture ◽  
Culture Test ◽  
Cell Culture Test

scholarly journals PREPARATION OF TRANSPARENT HYDROXYAPATITE CERAMICS BY SPARK PLASMA SINTERING AND CELL CULTURE TEST

2006 ◽  
Vol 20 ◽  
pp. 119-128 ◽  
Author(s):  
Daisuke Kawagoe ◽  
Yoshihiro Koga ◽  
Emile Hideki Ishida ◽  
Noriko Kotobuki ◽  
Hajime Ohgushi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Spark Plasma Sintering ◽  
Culture Test ◽  
Plasma Sintering ◽  
Hydroxyapatite Ceramics ◽  
Spark Plasma ◽  
Cell Culture Test
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