Automatic and label‐free identification of blood vessels in gliomas using the combination of multiphoton microscopy and image analysis

2019 ◽  
Vol 12 (9) ◽  
Author(s):  
Na Fang ◽  
Zanyi Wu ◽  
Xingfu Wang ◽  
Dezhi Kang ◽  
Lianhuang Li ◽  
...  
Keyword(s):  
Image Analysis ◽  
Blood Vessels ◽  
Multiphoton Microscopy ◽  
Label Free

scholarly journals Label-Free Identification of Early Gastrointestinal Neuroendocrine Tumors via Biomedical Multiphoton Microscopy and Automatic Image Analysis

IEEE Access ◽  
2020 ◽  
Vol 8 ◽  
pp. 105681-105689
Author(s):  
Lianhuang Li ◽  
Shenghui Huang ◽  
Lida Qiu ◽  
Weizhong Jiang ◽  
Zhifen Chen ◽  
...  
Keyword(s):  
Image Analysis ◽  
Neuroendocrine Tumors ◽  
Multiphoton Microscopy ◽  
Label Free ◽  
Automatic Image Analysis

scholarly journals Label-Free Imaging of Blood Vessels in Human Normal Breast and Breast Tumor Tissue Using Multiphoton Microscopy

Scanning ◽  
2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Gangqin Xi ◽  
Ning Cao ◽  
Wenhui Guo ◽  
Deyong Kang ◽  
Zhong Chen ◽  
...  
Keyword(s):  
Tumor Microenvironment ◽  
Blood Vessels ◽  
Breast Tumor ◽  
Tumor Tissue ◽  
Second Harmonic ◽  
Multiphoton Microscopy ◽  
Biological Tissues ◽  
Normal Breast ◽  
Label Free ◽  
Breast Tumor Tissue

Blood vessels are the important components of the circulatory systems that transport blood throughout the human body and maintain the homeostasis of physiological tissues. Pathologically, blood vessels are often affected by diseases, leading to the formation of unstable, irregular, and hyperpermeable blood vessels. In the tumor microenvironment, abnormal leakage of tumor blood vessels is related to the histological grade and malignant potential of tumors and may also facilitate metastasis of cancer. Visual diagnosis of blood vessels is very important for us to understand the occurrence and development of diseases. Multiphoton microscopy (MPM) is a potential label-free diagnostic tool based on second harmonic generation (SHG) and two-photon excited fluorescence (TPEF). MPM can effectively observe the morphological changes of biological tissues at the molecular and cellular levels. In this work, we demonstrate that label-free MPM can be used to visualize the microstructure of blood vessels in human normal breast and breast tumor tissue. Moreover, MPM can monitor the changes of blood vessels in tumor microenvironment. These results show that the MPM will become a promising technique for clinicians to study the properties of the microstructure of the blood vessels.

Label-Free Estimation of Therapeutic Efficacy on 3D Cancer Spheres Using Convolutional Neural Network Image Analysis

2019 ◽  
Vol 91 (21) ◽  
pp. 14093-14100 ◽  
Author(s):  
Zhixiong Zhang ◽  
Lili Chen ◽  
Yimin Wang ◽  
Tiantian Zhang ◽  
Yu-Chih Chen ◽  
...  
Keyword(s):  
Neural Network ◽  
Image Analysis ◽  
Convolutional Neural Network ◽  
Therapeutic Efficacy ◽  
Label Free

scholarly journals Label-free multiphoton microscopy reveals altered tissue architecture in hippocampal sclerosis

Epilepsia ◽  
2016 ◽  
Vol 58 (1) ◽  
pp. e1-e5 ◽  
Author(s):  
Ortrud Uckermann ◽  
Roberta Galli ◽  
Susann Leupold ◽  
Roland Coras ◽  
Matthias Meinhardt ◽  
...  
Keyword(s):  
Hippocampal Sclerosis ◽  
Multiphoton Microscopy ◽  
Label Free ◽  
Tissue Architecture

scholarly journals Label-free, three-dimensional multiphoton microscopy of the connective tissue in the anterior vaginal wall

2014 ◽  
Vol 26 (5) ◽  
pp. 685-691
Author(s):  
Michal Sikora ◽  
David Scheiner ◽  
Cornelia Betschart ◽  
Daniele Perucchini ◽  
José María Mateos ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Multiphoton Microscopy ◽  
Three Dimensional ◽  
Vaginal Wall ◽  
Anterior Vaginal Wall ◽  
Label Free

Correlative multiphoton microscopy and infrared nanospectroscopy of label-free collagen

2019 ◽  
Author(s):  
Jérémie Mathurin ◽  
Gervaise Mosser ◽  
Alexandre Dazzi ◽  
Ariane Deniset-Besseau ◽  
Marie-Claire Schanne-Klein ◽  
...  
Keyword(s):  
Multiphoton Microscopy ◽  
Label Free

Angiogenesis Assessed in Tissue Microarrays of Diagnostic Follicular Lymphoma Correlates with Outcome.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 2609-2609
Author(s):  
Andrew J. Clear ◽  
Abigail M. Lee ◽  
Kelly J. Morris ◽  
Maria Calaminici ◽  
Finlay Macdougall ◽  
...  
Keyword(s):  
Image Analysis ◽  
Confocal Microscopy ◽  
Follicular Lymphoma ◽  
Blood Vessels ◽  
Prognostic Value ◽  
Tissue Microarrays ◽  
Automated Image Analysis ◽  
Vessel Size ◽  
Sprouting Angiogenesis ◽  
Survivor Group

Abstract Micro-vessel density (MVD) is a powerful prognostic factor in cancers but its value in haematological malignancies is more controversial. To examine its prognostic value in follicular lymphoma (FL) we assessed number and distribution of blood vessels by high-throughput tissue microarray and automated image analysis measurements in tissue microarrays (TMA) of paraffin-embedded, diagnostic lymph node biopsies taken from fifty-nine FL patients. These patients were selected from those who died from lymphoma progression less than 5 years after diagnosis (short survivor group) (n=34) and those who survived more than 15 years from diagnosis (long survivor group) (n=25). Immunohistochemistry was used in TMAs to study the number and location of vessels staining positive for the endothelial cell markers CD31 and CD34 and pericyte coverage using PDGFR. Interactive quantification using image analysis software was used to provide details of absolute numbers of vessels from each patient, as well as vessel size and their location. Image stacks of immunofluorescence stained sections were obtained using laser-scanning confocal microscopy to trace the tumour vasculature. Results demonstrated that both total vessel count and mean vessel area were significantly different between the two groups. Samples from the long survivor group were significantly more likely to have fewer (p=0.025), but larger vessels (p=0.002) than those from the short surviving group. The differences in vessel size and number were more prominent in inter-follicular vessels compared with those inside the neoplastic follicles. The smaller and more numerous blood vessels seen in the poorer prognostic sub-group likely reflects active, sprouting angiogenesis as confirmed by confocal microscopy. This study validates the use of TMAs to examine angiogenesis and demonstrates the powerful prognostic value of assessing MVD in FL. These results suggest that sprouting angiogenesis represents a therapeutic target in this disease and ongoing studies are investigating the mechanisms contributing to alteration in angiogenesis in different prognostic subgroups in FL and in transformation of this disease. The prognostic significance of MVD assessment in TMA is currently being evaluated in a validation set of FL samples.

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