scholarly journals Angular range, sampling and noise considerations for inverse light scattering analysis of nuclear morphology

2018 ◽  
Vol 12 (2) ◽  
pp. e201800258 ◽  
Author(s):  
Haoran Zhang ◽  
Zachary A. Steelman ◽  
Derek S. Ho ◽  
Kengyeh K. Chu ◽  
Adam Wax
Keyword(s):  
Light Scattering ◽  
Nuclear Morphology ◽  
Angular Range

A light-scattering photometer for the angular range 6–170°

1968 ◽  
Vol 6 (3) ◽  
pp. 207-212 ◽  
Author(s):  
G. Meyerhoff ◽  
U. Moritz ◽  
R. L. Darskus
Keyword(s):  
Light Scattering ◽  
Angular Range

Light scattering from cervical cells throughout neoplastic progression: influence of nuclear morphology, DNA content, and chromatin texture

2003 ◽  
Vol 8 (1) ◽  
pp. 7 ◽  
Author(s):  
Rebekah Drezek ◽  
Martial Guillaud ◽  
Thomas Collier ◽  
Iouri Boiko ◽  
Anais Malpica ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dna Content ◽  
Nuclear Morphology ◽  
Neoplastic Progression ◽  
Chromatin Texture ◽  
Cervical Cells

Assessing Epithelial Cell Nuclear Morphology with Azimuthal Light Scattering Spectroscopy

2006 ◽  
Author(s):  
C. C. Yu ◽  
C. Lau ◽  
J. W. Tunnell ◽  
M. Hunter ◽  
M. Kalashnikov ◽  
...  
Keyword(s):  
Light Scattering ◽  
Epithelial Cell ◽  
Nuclear Morphology ◽  
Light Scattering Spectroscopy

Assessing epithelial cell nuclear morphology by using azimuthal light scattering spectroscopy

2006 ◽  
Vol 31 (21) ◽  
pp. 3119 ◽  
Author(s):  
Chung-Chieh Yu ◽  
Condon Lau ◽  
James W. Tunnell ◽  
Martin Hunter ◽  
Maxim Kalashnikov ◽  
...  
Keyword(s):  
Light Scattering ◽  
Epithelial Cell ◽  
Nuclear Morphology ◽  
Light Scattering Spectroscopy

A linearity test for thick specimens imaged by HVEM over a 180° angular range

1991 ◽  
Vol 49 ◽  
pp. 552-553
Author(s):  
Yu Liu
Keyword(s):  
Phase Contrast ◽  
Three Dimensional ◽  
Angular Range ◽  
Two Dimensional ◽  
Back Projection ◽  
Line Integral ◽  
Exponential Law ◽  
Transmission Electron ◽  
Absorption Law ◽  
Linearity Test

The image obtained in a transmission electron microscope is the two-dimensional projection of a three-dimensional (3D) object. The 3D reconstruction of the object can be calculated from a series of projections by back-projection, but this algorithm assumes that the image is linearly related to a line integral of the object function. However, there are two kinds of contrast in electron microscopy, scattering and phase contrast, of which only the latter is linear with the optical density (OD) in the micrograph. Therefore the OD can be used as a measure of the projection only for thin specimens where phase contrast dominates the image. For thick specimens, where scattering contrast predominates, an exponential absorption law holds, and a logarithm of OD must be used. However, for large thicknesses, the simple exponential law might break down due to multiple and inelastic scattering.

An adrenocortical oncocytoma with unusual mitochondrial inclusions and cytoplasmic crystals

1994 ◽  
Vol 52 ◽  
pp. 250-251
Author(s):  
W.T. Gunning ◽  
G.D. Haselhuhn ◽  
E.R. Phillips ◽  
S.H. Selman
Keyword(s):  
Adrenal Gland ◽  
Salivary Glands ◽  
Mitotic Activity ◽  
Diagnostic Criteria ◽  
Vascular Invasion ◽  
Benign Neoplasm ◽  
Benign Tumors ◽  
Nuclear Morphology ◽  
Case Presentation ◽  
Microscopic Evidence

Within the last few years, adrenal cortical tumors with features concordant with the diagnostic criteria attributed to oncocytomas have been reported. To date, only nine reported cases exist in the literature. This report is the tenth case presentation of a presumptively benign neoplasm of the adrenal gland with a rare differentiation. Oncocytomas are well recognized benign tumors of the thyroid, parathyroid, and salivary glands and of the kidney. Other organs also give rise to these types of tumors, however with less frequency than the former sites. The characteristics generally used to classify a tumor as an oncocytoma include the following criteria: the tumor is 1) usually a solitary circumscribed mass with no gross nor microscopic evidence of metastasis (no tissue nor vascular invasion), 2) fairly bland in terms of mitotic activity and nuclear morphology, and 3) composed of large eosinophillic cells in which the cytoplasm is packed full of mitochondria (Figure 1).

Mosaic Mapping: A Means of Tiling Images to Shorten Acquisition Speed for Lower - Magnification SEM Images and Wavelength Dispersive Spectrometers (WDS) X-Ray Maps

1996 ◽  
Vol 54 ◽  
pp. 438-439
Author(s):  
J.D. Geller ◽  
C.R. Herrington
Keyword(s):  
Limiting Factors ◽  
X Rays ◽  
Angular Range ◽  
Acceptance Angle ◽  
Sem Images ◽  
Worst Case ◽  
X Ray ◽  
Focusing Distance ◽  
Layered Crystals ◽  
Working Distance

The minimum magnification for which an image can be acquired is determined by the design and implementation of the electron optical column and the scanning and display electronics. It is also a function of the working distance and, possibly, the accelerating voltage. For secondary and backscattered electron images there are usually no other limiting factors. However, for x-ray maps there are further considerations. The energy-dispersive x-ray spectrometers (EDS) have a much larger solid angle of detection that for WDS. They also do not suffer from Bragg’s Law focusing effects which limit the angular range and focusing distance from the diffracting crystal. In practical terms EDS maps can be acquired at the lowest magnification of the SEM, assuming the collimator does not cutoff the x-ray signal. For WDS the focusing properties of the crystal limits the angular range of acceptance of the incident x-radiation. The range is dependent upon the 2d spacing of the crystal, with the acceptance angle increasing with 2d spacing. The natural line width of the x-ray also plays a role. For the metal layered crystals used to diffract soft x-rays, such as Be - O, the minimum magnification is approximately 100X. In the worst case, for the LEF crystal which diffracts Ti - Zn, ˜1000X is the minimum.

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