Improved label-free diagnostics and pathological assessment of atherosclerotic plaques through nonlinear microscopy

2018 ◽  
Vol 11 (11) ◽  
pp. e201800106 ◽  
Author(s):  
Enrico Baria ◽  
Gabriella Nesi ◽  
Raffaella Santi ◽  
Vincenza Maio ◽  
Daniela Massi ◽  
...  
Keyword(s):  
Atherosclerotic Plaques ◽  
Nonlinear Microscopy ◽  
Label Free ◽  
Pathological Assessment

Non-invasive label-free investigation and typing of head and neck cancers by multimodal nonlinear microscopy

2012 ◽  
Author(s):  
Tobias Meyer ◽  
Nadine Vogler ◽  
Benjamin Dietzek ◽  
Denis Akimov ◽  
Johanna Inhestern ◽  
...  
Keyword(s):  
Head And Neck ◽  
Head And Neck Cancers ◽  
Nonlinear Microscopy ◽  
Label Free ◽  
Non Invasive

scholarly journals Label-free imaging of age-related cardiac structural changes in non-human primates using multiphoton nonlinear microscopy

2021 ◽  
Author(s):  
Amara Khan ◽  
Fernanda Ramos-Gomes ◽  
Andrea Markus ◽  
Matthias Mietsch ◽  
Rabea Hinkel ◽  
...  
Keyword(s):  
Structural Changes ◽  
Nonlinear Microscopy ◽  
Label Free ◽  
Age Related

Melanin-targeted nonlinear microscopy for label-free molecular diagnosis and staging

2016 ◽  
Author(s):  
Warren S. Warren ◽  
Francisco Robles ◽  
Martin Fischer ◽  
Jesse Wilson ◽  
Sanghamitra Deb ◽  
...  
Keyword(s):  
Molecular Diagnosis ◽  
Nonlinear Microscopy ◽  
Label Free

scholarly journals Label-free imaging of atherosclerotic plaques using third-harmonic generation microscopy

2017 ◽  
Vol 9 (1) ◽  
pp. 214 ◽  
Author(s):  
David M. Small ◽  
Jason S. Jones ◽  
Irwin I. Tendler ◽  
Paul E. Miller ◽  
Andre Ghetti ◽  
...  
Keyword(s):  
Harmonic Generation ◽  
Third Harmonic Generation ◽  
Atherosclerotic Plaques ◽  
Label Free ◽  
Third Harmonic ◽  
Third Harmonic Generation Microscopy

Label-free live brain imaging with ultrafast nonlinear microscopy

2010 ◽  
Author(s):  
S. Witte ◽  
A. Negrean ◽  
J.C. Lodder ◽  
G.T. Silva ◽  
C.P.J. de Kock ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Nonlinear Microscopy ◽  
Label Free

scholarly journals Multimodal nonlinear microscopy: A powerful label-free method for supporting standard diagnostics on biological tissues

2014 ◽  
Vol 07 (05) ◽  
pp. 1330008 ◽  
Author(s):  
Riccardo Cicchi ◽  
Francesco Saverio Pavone
Keyword(s):  
Laser Scanning ◽  
Imaging Techniques ◽  
Biological Tissues ◽  
Laser Scanning Microscopy ◽  
Tissue Imaging ◽  
Nonlinear Microscopy ◽  
Label Free ◽  
Free Alternative ◽  
Functional Scheme ◽  
Nonlinear Imaging

The large use of nonlinear laser scanning microscopy in the past decade paved the way for potential clinical application of this imaging technique. Modern nonlinear microscopy techniques offer promising label-free solutions to improve diagnostic performances on tissues. In particular, the combination of multiple nonlinear imaging techniques in the same microscope allows integrating morphological with functional information in a morpho-functional scheme. Such approach provides a high-resolution label-free alternative to both histological and immunohistochemical examination of tissues and is becoming increasingly popular among the clinical community. Nevertheless, several technical improvements, including automatic scanning and image analysis, are required before the technique represents a standard diagnostic method. In this review paper, we highlight the capabilities of multimodal nonlinear microscopy for tissue imaging, by providing various examples on colon, arterial and skin tissues. The comparison between images acquired using multimodal nonlinear microscopy and histology shows a good agreement between the two methods. The results demonstrate that multimodal nonlinear microscopy is a powerful label-free alternative to standard histopathological methods and has the potential to find a stable place in the clinical setting in the near future.

scholarly journals Site-specific impairment of perivascular adipose tissue on advanced atherosclerotic plaques using multimodal nonlinear optical imaging

2019 ◽  
Vol 116 (36) ◽  
pp. 17765-17774 ◽  
Author(s):  
Suho Kim ◽  
Eun-Soo Lee ◽  
Sang-Won Lee ◽  
Yong-Hoon Kim ◽  
Chul-Ho Lee ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Nonlinear Optical ◽  
Brown Fat ◽  
Atherosclerotic Plaques ◽  
Label Free ◽  
Perivascular Adipose Tissue ◽  
Site Specific ◽  
Knockout Mouse Model ◽  
Initial Stage ◽  
Vascular Physiology

Perivascular adipose tissue (PVAT), as a mechanical support, has been reported to systemically regulate vascular physiology by secreting adipokines and cytokines. How PVAT spatially and locally changes as atherosclerosis progresses is not known, however. We aimed to reveal the molecular changes in PVAT in advanced atherosclerosis based on multimodal nonlinear optical (MNLO) imaging. First, using an atherogenic apolipoprotein E knockout mouse model, we precisely assessed the browning level of thoracic PVAT via a correlative analysis between the size and number of lipid droplets (LDs) of label-free MNLO images. We also biochemically demonstrated the increased level of brown fat markers in the PVAT of atherosclerosis. In the initial stage of atherosclerosis, the PVAT showed a highly activated brown fat feature due to the increased energy expenditure; however, in the advanced stage, only the PVAT in the regions of the atherosclerotic plaques, not that in the nonplaque regions, showed site-specific changes. We found that p-smad2/3 and TGF-β signaling enhanced the increase in collagen to penetrate the PVAT and the agglomeration of LDs only at the sites of atherosclerotic plaques. Moreover, atherosclerotic thoracic PVAT (tPVAT) was an increased inflammatory response. Taken together, our findings show that PVAT changes differentially from the initial stages to advanced stages of atherosclerosis and undergoes spatial impairment focused on atherosclerotic plaques. Our study may provide insight into the local control of PVAT as a therapeutic target.

Quantitative 3D molecular cutaneous absorption in human skin using label free nonlinear microscopy

2015 ◽  
Vol 200 ◽  
pp. 78-86 ◽  
Author(s):  
Xueqin Chen ◽  
Sébastien Grégoire ◽  
Florian Formanek ◽  
Jean-Baptiste Galey ◽  
Hervé Rigneault
Keyword(s):  
Human Skin ◽  
Nonlinear Microscopy ◽  
Label Free
Sign in / Sign up

Export Citation Format

Share Document