Optical reprogramming of human cells in an ultrashort femtosecond laser microfluidic transfection platform

2015 ◽  
Vol 9 (9) ◽  
pp. 942-947 ◽  
Author(s):  
Aisada Uchugonova ◽  
Hans Georg Breunig ◽  
Ana Batista ◽  
Karsten König
2013 ◽  
Vol 50 (8) ◽  
pp. 080011
Author(s):  
王艺森 Wang Yisen ◽  
贺号 He Hao ◽  
王清月 Wang Chingyue

2012 ◽  
Vol 15 (2) ◽  
pp. 279-288 ◽  
Author(s):  
Tarmo Nuutinen ◽  
Martti Silvennoinen ◽  
Kimmo Päiväsaari ◽  
Pasi Vahimaa

Author(s):  
Jun Zhang ◽  
Patrick Byers ◽  
Christine Frank ◽  
Levin Schulte-Spechtel ◽  
Bastian Hartmann ◽  
...  

2002 ◽  
Vol 72 (3) ◽  
pp. 147-153 ◽  
Author(s):  
Kei-Ichi Hirai ◽  
Jie-Hong Pan ◽  
Ying-Bo Shui ◽  
Eriko Simamura ◽  
Hiroki Shimada ◽  
...  

The possible protection of cultured human cells from acute dioxin injury by antioxidants was investigated. The most potent dioxin, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), caused vacuolization of the smooth endoplasmic reticulum and Golgi apparatus in cultured human conjunctival epithelial cells and cervical cancer cells. Subsequent nuclear damage included a deep irregular indentation resulting in cell death. A dosage of 30–40 ng/mL TCDD induced maximal intracellular production of H2O2 at 30 minutes and led to severe cell death (0–31% survival) at two hours. A dose of 1.7 mM alpha-tocopherol or 1 mM L-dehydroascorbic acid significantly protected human cells against acute TCDD injuries (78–97% survivals), but vitamin C did not provide this protection. These results indicate that accidental exposure to fatal doses of TCDD causes cytoplasmic free radical production within the smooth endoplasmic reticular systems, resulting in severe cytotoxicity, and that vitamin E and dehydroascorbic acid can protect against TCDD-induced cell damage.


2020 ◽  
Vol 92 (2) ◽  
pp. 20701
Author(s):  
Bo Li ◽  
Xiaofeng Li ◽  
Zhifeng Zhu ◽  
Qiang Gao

Laser-induced breakdown spectroscopy (LIBS) is a powerful technique for quantitative diagnostics of gases. The spatial resolution of LIBS, however, is limited by the volume of plasma. Here femtosecond-nanosecond dual-pulsed LIBS was demonstrated. Using this method, the breakdown threshold was reduced by 80%, and decay of continuous radiation was shortened. In addition, the volume of the plasma was shrunk by 85% and hence, the spatial resolution of LIBS was significantly improved.


2003 ◽  
Vol 104 ◽  
pp. 289-292 ◽  
Author(s):  
R. Ortega ◽  
B. Fayard ◽  
M. Salomé ◽  
G. Devès ◽  
J. Susini

2004 ◽  
Vol 36 (05) ◽  
Author(s):  
U Henning ◽  
K Krieger ◽  
S Loeffler ◽  
A Klimke
Keyword(s):  

2006 ◽  
Vol 223 (S 1) ◽  
Author(s):  
MP Holzer ◽  
TM Rabsilber ◽  
GU Auffarth

2015 ◽  
Author(s):  
Gerard Ruiz Babot ◽  
Irene Hadjidemetriou ◽  
Sharon Jane Ajodha ◽  
David Taylor ◽  
Norman Taylor ◽  
...  

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