scholarly journals In vivoblood flow imaging of inflammatory human skin induced by tape stripping using optical microangiography

2014 ◽  
Vol 8 (3) ◽  
pp. 265-272 ◽  
Author(s):  
Hequn Wang ◽  
Utku Baran ◽  
Ruikang K. Wang
Keyword(s):  
Human Skin ◽  
Tape Stripping ◽  
Flow Imaging ◽  
Optical Microangiography

scholarly journals Comparison of tape stripping with the human skin blanching assay for the bioequivalence assessment of topical clobetasol propionate formulations.

2010 ◽  
Vol 13 (1) ◽  
pp. 11 ◽  
Author(s):  
Wai Ling Au ◽  
Michael F Skinner ◽  
Isadore Kanfer
Keyword(s):  
Topical Corticosteroid ◽  
Human Skin ◽  
The United States ◽  
Clobetasol Propionate ◽  
Tape Stripping ◽  
Draft Guidance ◽  
Ointment Formulation ◽  
United States Food ◽  
Assay Methods ◽  
States Food

Purpose: A draft guidance on tape stripping for assessing the bioavailability/bioequivalence of topical formulations was issued by the United States Food and Drug Administration in 1998 but has since been withdrawn. This was due to problems associated with the method and also inconsistencies and variability in the resulting data. The purpose of this study was to re-visit the tape stripping technique, incorporate refinements to reduce variability and validate the method using bioequivalence data obtained from the assessment of a topical corticosteroid cream containing 0.05% clobetasol propionate using the human skin-blanching assay. Methods: A pilot tape stripping study was conducted to establish the variability of the formulations.The bioequivalence of two different commercially available clobetasol propionate cream formulations and a clobetasol propionate ointment formulation were subsequently investigated using the tape stripping method. Results: The data from the pilot tape stripping study correlated well with data from the human skin-blanching assay. A subsequent pivotal tape stripping study confirmed bioequivalence between the two cream formulations whereas bio-inequivalence was demonstrated between the cream and ointment formulations.Conclusions: These studies show that the results from tape stripping concur with data from the human skin blanching assay and demonstrate the potential of a well-controlled tape stripping study as an option for the assessment of bioequivalence of topical corticosteroid formulations.

scholarly journals IL-23 induced in keratinocytes by endogenous TLR4 ligands polarizes dendritic cells to drive IL-22 responses to skin immunization

2016 ◽  
Vol 213 (10) ◽  
pp. 2147-2166 ◽  
Author(s):  
Juhan Yoon ◽  
Juan Manuel Leyva-Castillo ◽  
Guoxing Wang ◽  
Claire Galand ◽  
Michiko K. Oyoshi ◽  
...  
Keyword(s):  
T Cells ◽  
Human Skin ◽  
Cd4 T Cells ◽  
Mouse Skin ◽  
Skin Inflammation ◽  
Serum Levels ◽  
Skin Lesions ◽  
Tape Stripping ◽  
Keratinocyte Proliferation ◽  
Th22 Cells

Atopic dermatitis (AD) is a Th2-dominated inflammatory skin disease characterized by epidermal thickening. Serum levels of IL-22, a cytokine known to induce keratinocyte proliferation, are elevated in AD, and Th22 cells infiltrate AD skin lesions. We show that application of antigen to mouse skin subjected to tape stripping, a surrogate for scratching, induces an IL-22 response that drives epidermal hyperplasia and keratinocyte proliferation in a mouse model of skin inflammation that shares many features of AD. DC-derived IL-23 is known to act on CD4+ T cells to induce IL-22 production. However, the mechanisms that drive IL-23 production by skin DCs in response to cutaneous sensitization are not well understood. We demonstrate that IL-23 released by keratinocytes in response to endogenous TLR4 ligands causes skin DCs, which selectively express IL-23R, to up-regulate their endogenous IL-23 production and drive an IL-22 response in naive CD4+ T cells that mediates epidermal thickening. We also show that IL-23 is released in human skin after scratching and polarizes human skin DCs to drive an IL-22 response, supporting the utility of IL-23 and IL-22 blockade in AD.

scholarly journals Tape stripping method is useful for the quantification of antimicrobial peptides on the human skin surface including the stratum corneum

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shigeyuki Ono ◽  
Nobuhiko Eda ◽  
Takuya Mori ◽  
Atsuko Otsuka ◽  
Nobuhiro Nakamura ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Stratum Corneum ◽  
Human Skin ◽  
Significant Positive Correlation ◽  
Skin Surface ◽  
Tape Stripping ◽  
Established Method ◽  
Positive Correlation ◽  
Physiological Importance ◽  
Stripping Method

Abstract Antimicrobial peptides (AMPs) play an important role in innate immunity in human skin. It is known that AMPs mainly function in the stratum corneum. Therefore, AMP concentrations in the stratum corneum need to be precisely measured to clarify functional and physiological importance of AMPs in cutaneous defence. Tape stripping (TS) is a well-established method by which components in the stratum corneum can be collected. However, the usefulness of the TS method for measuring AMP concentration in human skin remains unclear. Therefore, we compared it with another popular method, skin rinsing, which had been established as a method for measuring AMP concentration in human skin. When investigated on healthy medial forearm using RNase 7, which is one of the typical AMPs, as an index, there was a significant positive correlation between RNase 7 concentrations measured by the TS method at adjacent forearm sites, demonstrating the reproducibility of the TS method. Next, a significant positive correlation was detected in RNase 7 concentrations measured using the TS and the skin rinsing method, indicating that the TS method is comparable to the skin rinsing method. Thus, we speculate that the TS method is useful for measuring AMP concentration in human skin.

scholarly journals Methods and Limits for Micro Scale Blood Vessel Flow Imaging in Scattering Media by Optical Feedback Interferometry: Application to Human Skin

Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1300 ◽  
Author(s):  
Adam Quotb ◽  
Reza Atashkhooei ◽  
Simone Magaletti ◽  
Francis Jayat ◽  
Clement Tronche ◽  
...  
Keyword(s):  
Blood Flow ◽  
Human Skin ◽  
Network Architecture ◽  
Health Management ◽  
Optical Feedback ◽  
Flow Imaging ◽  
Scattering Media ◽  
In Vivo Test

At the micrometric scale, vessels or skin capillaries network architecture can provide useful information for human health management. In this paper, from simulation to in vitro, we investigate some limits and interests of optical feedback interferometry (OFI) for blood flow imaging of skin vascularization. In order to analyze the tissue scattering effect on OFI performances, a series of skin-tissue simulating optical phantoms have been designed, fabricated and characterized. The horizontal (2D) and vertical (depth penetration) sensing resolution of the OFI sensor have been estimated. The experimental results that we present on this study are showing a very good accordance with theoretical models. In the case of a skin phantom of 0.5 mm depth with a scattering coefficient from 0 to 10.8 mm−1, the presented OFI system is able to distinguish a pair of micro fluidic channels (100 µm × 100 µm) spaced by 10 µm. Eventually, an in vivo test on human skin is presented and, for the first time using an OFI sensor, a 2D blood flow image of a vein located just beneath the skin is computed.

scholarly journals Interpretation of the human skin biotribological behaviour after tape stripping

2011 ◽  
Vol 8 (60) ◽  
pp. 934-941 ◽  
Author(s):  
C. Pailler-Mattei ◽  
C. Guerret-Piécourt ◽  
H. Zahouani ◽  
S. Nicoli
Keyword(s):  
Shear Strength ◽  
Friction Coefficient ◽  
Skin Friction ◽  
Human Skin ◽  
Dielectric Material ◽  
Experimental System ◽  
Skin Surface ◽  
Skin Friction Coefficient ◽  
Tape Stripping ◽  
Adhesion Component

The present study deals with the modification of the human skin biotribological behaviour after tape stripping. The tape-stripping procedure consists in the sequential application and removal of adhesive tapes on the skin surface in order to remove stratum corneum (SC) layers, which electrically charges the skin surface. The skin electric charges generated by tape stripping highly change the skin friction behaviour by increasing the adhesion component of the skin friction coefficient. It has been proposed to rewrite the friction adhesion component as the sum of two terms: the first classical adhesion term depending on the intrinsic shear strength, τ 0 , and the second term depending on the electric shear strength, τ elec . The experimental results allowed to estimate a numerical value of the electric shear strength τ elec . Moreover, a plan capacitor model with a dielectric material inside was used to modelize the experimental system. This physical model permitted to evaluate the friction electric force and the electric shear strength values to calculate the skin friction coefficient after the tape stripping. The comparison between the experimental and the theoretical value of the skin friction coefficient after the tape stripping has shown the importance of the electric charges on skin biotribological behaviour. The static electric charges produced by tape stripping on the skin surface are probably able to highly modify the interaction of formulations with the skin surface and their spreading properties. This phenomenon, generally overlooked, should be taken into consideration as it could be involved in alteration of drug absorption.

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