Evaluation of zebrafish brain development using optical coherence tomography

2012 ◽  
Vol 6 (9) ◽  
pp. 668-678 ◽  
Author(s):  
Yu-Sheng Lin ◽  
Chin-Chou Chu ◽  
Po-Hsiang Tsui ◽  
Chien-Cheng Chang
Keyword(s):  
Optical Coherence Tomography ◽  
Brain Development ◽  
Optical Coherence ◽  
Zebrafish Brain

scholarly journals In Xenopus ependymal cilia drive embryonic CSF circulation and brain development independently of cardiac pulsatile forces

2020 ◽  
Vol 17 (1) ◽  
Author(s):  
A. H. Dur ◽  
T. Tang ◽  
S. Viviano ◽  
A. Sekuri ◽  
H. R. Willsey ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Brain Development ◽  
Neural Cell ◽  
Optical Coherence ◽  
Csf Flow ◽  
Csf Circulation ◽  
Cell Organization ◽  
Ependymal Cilia ◽  
Oct Imaging

Abstract Background Hydrocephalus, the pathological expansion of the cerebrospinal fluid (CSF)-filled cerebral ventricles, is a common, deadly disease. In the adult, cardiac and respiratory forces are the main drivers of CSF flow within the brain ventricular system to remove waste and deliver nutrients. In contrast, the mechanics and functions of CSF circulation in the embryonic brain are poorly understood. This is primarily due to the lack of model systems and imaging technology to study these early time points. Here, we studied embryos of the vertebrate Xenopus with optical coherence tomography (OCT) imaging to investigate in vivo ventricular and neural development during the onset of CSF circulation. Methods Optical coherence tomography (OCT), a cross-sectional imaging modality, was used to study developing Xenopus tadpole brains and to dynamically detect in vivo ventricular morphology and CSF circulation in real-time, at micrometer resolution. The effects of immobilizing cilia and cardiac ablation were investigated. Results In Xenopus, using OCT imaging, we demonstrated that ventriculogenesis can be tracked throughout development until the beginning of metamorphosis. We found that during Xenopus embryogenesis, initially, CSF fills the primitive ventricular space and remains static, followed by the initiation of the cilia driven CSF circulation where ependymal cilia create a polarized CSF flow. No pulsatile flow was detected throughout these tailbud and early tadpole stages. As development progressed, despite the emergence of the choroid plexus in Xenopus, cardiac forces did not contribute to the CSF circulation, and ciliary flow remained the driver of the intercompartmental bidirectional flow as well as the near-wall flow. We finally showed that cilia driven flow is crucial for proper rostral development and regulated the spatial neural cell organization. Conclusions Our data support a paradigm in which Xenopus embryonic ventriculogenesis and rostral brain development are critically dependent on ependymal cilia-driven CSF flow currents that are generated independently of cardiac pulsatile forces. Our work suggests that the Xenopus ventricular system forms a complex cilia-driven CSF flow network which regulates neural cell organization. This work will redirect efforts to understand the molecular regulators of embryonic CSF flow by focusing attention on motile cilia rather than other forces relevant only to the adult.

scholarly journals Real-timein vivoimaging of adult Zebrafish brain using optical coherence tomography

2009 ◽  
Vol 2 (5) ◽  
pp. 288-291 ◽  
Author(s):  
K. Divakar Rao ◽  
Aneesh Alex ◽  
Yogesh Verma ◽  
Sreeja Thampi ◽  
Pradeep K. Gupta
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence ◽  
Adult Zebrafish ◽  
Zebrafish Brain

1086: Cystoscopic Optical Coherence Tomography in Diagnosis and Management of Clinical Bladder Tumors

2007 ◽  
Vol 177 (4S) ◽  
pp. 358-359 ◽  
Author(s):  
Christopher S. Lee ◽  
Alek Mishail ◽  
Jason M. Kim ◽  
Alexander Kirshenbaum ◽  
Howard L. Adler ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence ◽  
Bladder Tumors ◽  
Diagnosis And Management

1227: Optical Coherence Tomography-Guided Surgery of Bladder Cancer

2006 ◽  
Vol 175 (4S) ◽  
pp. 395-395
Author(s):  
Nancy J. Tresser ◽  
Elena V. Zagaynova ◽  
Olga S. Streltsova ◽  
Natalia D. Gladkova ◽  
Vladislav A. Kamensky ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Optical Coherence Tomography ◽  
Optical Coherence ◽  
Guided Surgery
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