In vitro and in vivo metabolic stability of various fragrance materials and insect repellent in rainbow trout ( Oncorhynchus mykiss )

2020 ◽  
Vol 40 (6) ◽  
pp. 763-779 ◽  
Author(s):  
John Weeks ◽  
Patrick Guiney ◽  
Karla Johanning
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Metabolic Stability ◽  
Insect Repellent ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Fragrance Materials

Rancidity development of refrigerated rainbow trout ( Oncorhynchus mykiss ) fillets: comparative effects of in vivo and in vitro lycopene

2017 ◽  
Vol 98 (2) ◽  
pp. 559-565 ◽  
Author(s):  
Ali Ehsani ◽  
Mohammad Sedigh Jasour ◽  
Naser Agh ◽  
Mohammad Hashemi ◽  
Mahdi Khodadadi
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Rainbow Trout Oncorhynchus Mykiss

In vivo and in vitro liver and gill EROD activity in rainbow trout (Oncorhynchus mykiss) exposed to the beta-blocker propranolol

2011 ◽  
Vol 27 (10) ◽  
pp. 573-582 ◽  
Author(s):  
Abigail E. Bartram ◽  
Matthew J. Winter ◽  
Duane B. Huggett ◽  
Paul McCormack ◽  
Lisa A. Constantine ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Beta Blocker ◽  
Erod Activity ◽  
Rainbow Trout Oncorhynchus Mykiss

scholarly journals Genistein Induces Adipogenic and Autophagic Effects in Rainbow Trout (Oncorhynchus mykiss) Adipose Tissue: In Vitro and In Vivo Models

2020 ◽  
Vol 21 (16) ◽  
pp. 5884
Author(s):  
Sara Balbuena-Pecino ◽  
Esmail Lutfi ◽  
Natàlia Riera-Heredia ◽  
Esther Gasch-Navalón ◽  
Emilio J. Vélez ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Rainbow Trout ◽  
Lipid Metabolism ◽  
Oncorhynchus Mykiss ◽  
Fatty Acid Synthase ◽  
Hormone Sensitive Lipase ◽  
In Vivo Models ◽  
Rainbow Trout Oncorhynchus Mykiss

Soybeans are one of the most used alternative dietary ingredients in aquafeeds. However, they contain phytoestrogens like genistein (GE), which can have an impact on fish metabolism and health. This study aimed to investigate the in vitro and in vivo effects of GE on lipid metabolism, apoptosis, and autophagy in rainbow trout (Oncorhynchus mykiss). Primary cultured preadipocytes were incubated with GE at different concentrations, 10 or 100 μM, and 1 μM 17β-estradiol (E2). Furthermore, juveniles received an intraperitoneal injection of GE at 5 or 50 µg/g body weight, or E2 at 5 µg/g. In vitro, GE 100 μM increased lipid accumulation and reduced cell viability, apparently involving an autophagic process, indicated by the higher LC3-II protein levels, and higher lc3b and cathepsin d transcript levels achieved after GE 10 μM. In vivo, GE 50 µg/g upregulated the gene expression of fatty acid synthase (fas) and glyceraldehyde-3-phosphate dehydrogenase in adipose tissue, suggesting enhanced lipogenesis, whereas it increased hormone-sensitive lipase in liver, indicating a lipolytic response. Besides, autophagy-related genes increased in the tissues analyzed mainly after GE 50 µg/g treatment. Overall, these findings suggest that an elevated GE administration could lead to impaired adipocyte viability and lipid metabolism dysregulation in rainbow trout.

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