scholarly journals Searching for tryptase in the RBL-2H3 mast cell model: Preparation for comparative mast cell toxicology studies with zebrafish

2018 ◽  
Vol 39 (3) ◽  
pp. 473-484
Author(s):  
Juyoung K. Shim ◽  
Rachel H. Kennedy ◽  
Lisa M. Weatherly ◽  
Andrew V. Abovian ◽  
Hina N. Hashmi ◽  
...  
Keyword(s):  
Mast Cell ◽  
Cell Model ◽  
Model Preparation

scholarly journals Anti-Allergic Potential of Cinnamaldehyde via the Inhibitory Effect of Histidine Decarboxylase (HDC) Producing Klebsiella pneumonia

Molecules ◽  
2020 ◽  
Vol 25 (23) ◽  
pp. 5580
Author(s):  
Lorina I. Badger-Emeka ◽  
Promise Madu Emeka ◽  
Krishnaraj Thirugnanasambantham ◽  
Hairul Islam M. Ibrahim
Keyword(s):  
Mast Cell ◽  
Cell Activation ◽  
Histidine Decarboxylase ◽  
Cell Model ◽  
In Vitro Study ◽  
Inhibitory Effect ◽  
Mast Cell Activation ◽  
Klebsiella Pneumonia ◽  
Immunological Disorder

Allergy is an immunological disorder that develops in response to exposure to an allergen, and histamines mediate these effects via histidine decarboxylase (HDC) activity at the intracellular level. In the present study, we developed a 3D model of Klebsiella pneumoniae histidine decarboxylase (HDC) and analyzed the HDC inhibitory potential of cinnamaldehyde (CA) and subsequent anti-allergic potential using a bacterial and mammalian mast cell model. A computational and in vitro study using K. pneumonia revealed that CA binds to HDC nearby the pyridoxal-5′-phosphate (PLP) binding site and inhibited histamine synthesis in a bacterial model. Further study using a mammalian mast cell model also showed that CA decreased the levels of histamine in the stimulated RBL-2H3 cell line and attenuated the release of β-hexoseaminidase and cell degranulation. In addition, CA treatment also significantly suppressed the levels of pro-inflammatory cytokines TNF-α and IL-6 and the nitric oxide (NO) level in the stimulated mast cells. A gene expression and Western blotting study revealed that CA significantly downregulated the expressions of MAPKp38/ERK and its downstream pro-allergic mediators that are involved in the signaling pathway in mast cell cytokine synthesis. This study further confirms that CA has the potential to attenuate mast cell activation by inhibiting HDC and modifying the process of allergic disorders.

scholarly journals A novel KIT-deficient mouse mast cell model for the examination of human KIT-mediated activation responses

2013 ◽  
Vol 390 (1-2) ◽  
pp. 52-62 ◽  
Author(s):  
Daniel Smrž ◽  
Geethani Bandara ◽  
Shuling Zhang ◽  
Beverly A. Mock ◽  
Michael A. Beaven ◽  
...  
Keyword(s):  
Mast Cell ◽  
Cell Model ◽  
Deficient Mouse ◽  
Mouse Mast Cell

scholarly journals Stimulatory effect of cytochalasin D on antigen-induced phospholipase D activation in a murine mast cell model (RBL-2H3)

1999 ◽  
Vol 48 (4) ◽  
pp. 239-245
Author(s):  
Yoshikatsu Nakamura ◽  
Shigeru Nakashima ◽  
Katsuhiro Ojio ◽  
Yatsuji Ito ◽  
Kazuki Hayakawa ◽  
...  
Keyword(s):  
Mast Cell ◽  
Phospholipase D ◽  
Cell Model ◽  
Cytochalasin D ◽  
Murine Mast Cell

scholarly journals Viridicatol Isolated from Deep-Sea Penicillium Griseofulvum Alleviates Anaphylaxis and Repairs the Intestinal Barrier in Mice by Suppressing Mast Cell Activation

Marine Drugs ◽  
2020 ◽  
Vol 18 (10) ◽  
pp. 517
Author(s):  
Zhendan Shu ◽  
Qingmei Liu ◽  
Cuiping Xing ◽  
Yafen Zhang ◽  
Yu Zhou ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mouse Model ◽  
Deep Sea ◽  
Cell Activation ◽  
Immunoglobulin E ◽  
Cell Model ◽  
Intestinal Barrier ◽  
Interleukin 10 ◽  
Mast Cell Activation ◽  
Penicillium Griseofulvum

Viridicatol is a quinoline alkaloid isolated from the deep-sea-derived fungus Penicillium griseofulvum. The structure of viridicatol was unambiguously established by X-ray diffraction analysis. In this study, a mouse model of ovalbumin-induced food allergy and the rat basophil leukemia (RBL)-2H3 cell model were established to explore the anti-allergic properties of viridicatol. On the basis of the mouse model, we found viridicatol to alleviate the allergy symptoms; decrease the levels of specific immunoglobulin E, mast cell protease-1, histamine, and tumor necrosis factor-α; and promote the production of interleukin-10 in the serum. The treatment of viridicatol also downregulated the population of B cells and mast cells (MCs), as well as upregulated the population of regulatory T cells in the spleen. Moreover, viridicatol alleviated intestinal villi injury and inhibited the degranulation of intestinal MCs to promote intestinal barrier repair in mice. Furthermore, the accumulation of Ca2+ in RBL-2H3 cells was significantly suppressed by viridicatol, which could block the activation of MCs. Taken together, these data indicated that deep-sea viridicatol may represent a novel therapeutic for allergic diseases.

scholarly journals Transcriptional and Functional Plasticity Induced by Chronic Insulin Exposure in a Mast Cell-Like Basophilic Leukemia Cell Model

2017 ◽  
Vol 02 (04) ◽  
Author(s):  
Chad Jansen ◽  
Mark Speck ◽  
William E Greineisen ◽  
Kristina Maaetoft-Udsen ◽  
Edward Cordasco ◽  
...  
Keyword(s):  
Mast Cell ◽  
Cell Model ◽  
Leukemia Cell ◽  
Functional Plasticity ◽  
Basophilic Leukemia Cell ◽  
Basophilic Leukemia

Influence of the anti-allergic drug oxatomide on the signal transduction mechanism in a mast cell model

1996 ◽  
Vol 312 (1) ◽  
pp. 121-130 ◽  
Author(s):  
Jeannette J.C. Paulussen ◽  
Marcel J.E. Fischer ◽  
Jolien A.E. Kok-Van Esterik ◽  
Radboud C. Tiemessen ◽  
Nico J. De Mol ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Mast Cell ◽  
Cell Model ◽  
Transduction Mechanism ◽  
Signal Transduction Mechanism
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