Changes in serum?2u-globulin levels in castrated male rats treated with testosterone propionate in a Hershberger assay

2005 ◽  
Vol 25 (2) ◽  
pp. 176-178 ◽  
Author(s):  
Masahiro Takeyoshi ◽  
Hideo Mitoma ◽  
Kanji Yamasaki
Keyword(s):  
Testosterone Propionate ◽  
Male Rats ◽  
Hershberger Assay

scholarly journals Altered sexual differentiation of hepatic uridine diphosphate glucuronyltransferase by neonatal hormone treatment in rats

1979 ◽  
Vol 180 (2) ◽  
pp. 313-318 ◽  
Author(s):  
Coral A. Lamartiniere ◽  
Cindy S. Dieringer ◽  
Etsuko Kita ◽  
George W. Lucier
Keyword(s):  
Enzyme Activity ◽  
Adult Male ◽  
Sexual Differentiation ◽  
Reproductive Tract ◽  
Testosterone Propionate ◽  
Hormone Treatment ◽  
Male Rats ◽  
Ventral Prostate ◽  
Uridine Diphosphate ◽  
Neonatal Administration

The hepatic microsomal enzyme UDP-glucuronyltransferase undergoes a complex developmental pattern in which enzyme activity is first detectable on the 18th day of gestation in rats. Prepubertal activities are similar for males and females. However, postpubertal sexual differentiation of enzyme activity occurs in which male activities are twice those of females. Neonatal administration of testosterone propionate or diethylstilboestrol to intact animals resulted in lowered UDP-glucuronyltransferase activity in liver microsomal fractions of adult male rats, whereas no changes were observed in the adult females and prepubertal male and female animals. Neonatal administration of testosterone propionate and diethylstilboestrol adversely affected male reproductive-tract development as evidenced by decreased weights of testes, seminal vesicles and ventral prostate. Diethylstilboestrol also markedly decreased spermatogenesis. Hypophysectomy of adult male rats resulted in negative modulation of microsomal UDP-glucuronyltransferase and prevented the sexual differentiation of enzyme activity. In contrast hypophysectomy had no effect on female UDP-glucuronyltransferase activity. A pituitary transplant under the kidney capsule was not capable of reversing the enzyme effects of hypophysectomy, therefore suggesting that the male pituitary factor(s) responsible for positive modulation of UDP-glucuronyltransferase might be under hypothalamic control in the form of a releasing factor. Neonatal testosterone propionate and diethylstilboestrol administration apparently interfered with the normal sequence of postpubertal UDP-glucuronyltransferase sexual differentiation.

scholarly journals Different mechanisms of regulation of nuclear reduced nicotinamide–adenine dinucleotide phosphate-dependent 3-oxo steroid 5α-reductase activity in rat liver, kidney and prostate

1974 ◽  
Vol 142 (2) ◽  
pp. 273-277 ◽  
Author(s):  
Jan-Åke Gustafsson ◽  
Åke Pousette
Keyword(s):  
Testosterone Propionate ◽  
Reductase Activity ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Female Rats ◽  
Male Rats ◽  
Regulatory Mechanisms ◽  
Oestradiol Benzoate ◽  
Liver And Kidney ◽  
Reduced Nicotinamide Adenine Dinucleotide ◽  
Hydroxy Steroid

The regulatory mechanisms involved in the control of the nuclear NADPH-dependent 3-ketosteroid 5α-reductase (5α-reductase) activity were studied in liver, kidney and prostate. The substrate used was [1,2-3H]androst-4-ene-3,17-dione (androstenedione) (for liver and kidney) or [4-14C]androstenedione (for prostate). The hepatic nuclear 5α-reductase activity was greater in female than in male rats, was greater in adult than in prepubertal female rats, increased after castration of male rats, but was not affected by treatment with testosterone propionate or oestradiol benzoate. These regulatory characteristics are in part different from those previously described for the hepatic microsomal 5α-reductase. The renal nuclear metabolism of androstenedione, i.e. 5α reduction and 17β-hydroxy steroid reduction, was relatively unaffected by sex, age, castration and treatment with testosterone propionate. However, treatment of castrated male rats with oestradiol benzoate led to a significant increase in the 5α-reductase activity and a significant decrease in the 17β-hydroxy steroid reductase activity. Finally, the nuclear 5α-reductase activity in prostate was androgen-dependent, decreasing after castration and increasing after treatment with testosterone propionate. In conclusion, the nuclear 5α-reductase activities in liver, kidney and prostate seem to be under the control of distinctly different regulatory mechanisms. The hypothesis is presented that whereas the prostatic nuclear 5α-reductase participates in the formation of a physiologically active androgen, 5α-dihydrotestosterone, this may not be the true function of the nuclear 5α-reductase in liver and kidney. These enzymes might rather serve to protect the androgen target sites in the chromatin from active androgens (e.g. testosterone) by transforming them into less active androgens (e.g. 5α-androstane-3,17-dione and/or 5α-dihydrotestosterone).

THE EFFECT OF CERTAIN ANDROGENIC STEROIDS ON THE UPTAKE OF RADIOSULPHUR IN A HEALING FRACTURED BONE IN THE RAT

1957 ◽  
Vol 35 (1) ◽  
pp. 771-776 ◽  
Author(s):  
K. Kowalewski ◽  
R. T. Morrison
Keyword(s):  
Normal Control ◽  
Marked Effect ◽  
Testosterone Propionate ◽  
Chondroitin Sulphate ◽  
Significant Rise ◽  
Male Rats ◽  
Testosterone Administration ◽  
Androgenic Steroids ◽  
Intact Rats

The uptake of radiosulphur in the fractured and intact humerus of male rats was determined in normal and in castrated animals and the ratio F/I of radioactivity of fractured (F) to intact (I) bone was calculated. Castration resulted in a significant decrease of F/I ratio, as compared with normal control rats.The effect of testosterone propionate and 17-ethyl-19 nortestosterone (Nilevar) on the S35 uptake in bones was studied. Testosterone administration resulted in a slight increase of the F/I in castrated rats, but had no effect on this ratio in intact animals. Both castrated and intact rats treated with Nilevar exhibited a significant rise of S35 uptake in fractured bones and, consequently, high F/I ratios. It is suggested that Nilevar has a marked effect on the synthesis of chondroitin sulphate in the collagen tissues of healing fractures, as measured by the described S35 uptake technique.

Effects of intrahypothalamic crystalline steroids on acute ACTH secretion

1963 ◽  
Vol 205 (4) ◽  
pp. 671-673 ◽  
Author(s):  
Israel Chowers ◽  
Shaul Feldman ◽  
Julian M. Davidson
Keyword(s):  
Ascorbic Acid ◽  
Pituitary Gland ◽  
Median Eminence ◽  
Acute Stress ◽  
Testosterone Propionate ◽  
Experimental Period ◽  
Male Rats ◽  
Acth Secretion ◽  
Unilateral Adrenalectomy ◽  
Cortisol Implants

The respective roles of the hypothalamus and pituitary gland, in the inhibition of adrenocorticotropin secretion by corticoids, were studied by implanting small quantities of crystalline cortisol acetate in the median eminence region and pituitary of male rats. Adrenal weights and adrenal ascorbic acid depletion (AAAD) in response to the acute stress of unilateral adrenalectomy were measured 5, 10, 13, or 21 days postoperatively. Ten days following implantation in the hypothalamus, rats showed adrenal atrophy and inhibition of AAAD. Normal AAAD and slight adrenal hypertrophy were found 10 days after similar implantation of testosterone propionate in the median eminence. Animals with cortisol implants in the pituitary had normal adrenal weights and AAAD responses at this time. In rats with cortisol implants in the hypothalamus, an inhibition of AAAD was present after 5–6 days, had increased maximally at 13 days, and returned to normal at 21 days. Adrenal atrophy, however, was first noted at 10 days and adrenal weight continued to decline throughout the experimental period.

Synergism of testosterone propionate with growth hormone in promoting growth of hypophysectomized rats: effect of sexual differentiation

1990 ◽  
Vol 127 (2) ◽  
pp. 249-256 ◽  
Author(s):  
J. Klindt ◽  
J. J. Ford ◽  
G. J. Macdonald
Keyword(s):  
Sexual Differentiation ◽  
Testosterone Propionate ◽  
Initial Study ◽  
Male Rats ◽  
Growth Enhancement ◽  
Growth Responses ◽  
Liver And Kidney ◽  
Hypophysectomized Rats ◽  
Synergistic Response ◽  
Rates Of Growth

ABSTRACT The effect of testosterone propionate (TP), alone and in combination with porcine GH, on the growth of hypophysectomized rats was investigated. An initial study determined doses of TP and GH which would result in a synergistic response. Hypophysectomized male rats, approximately 40 days of age, received GH at doses of 5, 25 and 62·5 μg/day administered in two injections/day at 08.00 and 16.00 h. At all doses of GH, administration of TP at 100 μg/day significantly enhanced the GH-stimulated rate of growth. This growth enhancement by TP was greatest in combination with GH at 25 μg/day. In a subsequent study, growth responses to 25 μg GH/day and 100 μg TP/day were examined in animals with differing degrees of sexual differentiation. Sex groups were: intact males, males castrated at 11 days of age and females administered 100 μg TP at 3 days of age (masculinized rats), and males castrated at 2 days of age and normal females (non-masculinized rats). In all sex groups, growth of hypophysectomized rats was stimulated by GH. Genetic sex and masculinization did not influence the response to GH. Masculinized hypophysectomized rats exhibited significantly greater rates of growth and final live, empty body, liver and kidney weights than non-masculinized hypophysectomized rats. All sex groups other than normal females responded synergistically to the combination treatment of GH plus TP. Rats that experienced neonatal exposure to testosterone became programmed to respond to testosterone and demonstrated greater rates of growth and body and organ weights when administered the combination of GH plus TP. These data indicate that TP synergizes with GH to promote growth of hypophysectomized rats appropriately programmed to respond. The ability to manifest a synergistic response is a differentiated trait dependent upon exposure to testosterone during the appropriate period of development. The time of differentiation of this ability to respond to testosterone occurs earlier than that for differentiation of body growth. Journal of Endocrinology (1990) 127, 249–256

EFFECTS OF TESTOSTERONE AND NANDROLONE AND SOME OF THEIR ESTERS ON THE PSEUDOCHOLINESTERASE ACTIVITY IN THE LIVER AND SERUM AND ON THE SEMINAL VESICLE AND LEVATOR ANI MUSCLE OF THE RAT

1970 ◽  
Vol 64 (3) ◽  
pp. 531-540 ◽  
Author(s):  
R. S. Leeuwin ◽  
E. Th. Groenewoud
Keyword(s):  
Enzyme Activity ◽  
Seminal Vesicle ◽  
Testosterone Propionate ◽  
Levator Ani ◽  
Daily Administration ◽  
Male Rats ◽  
Levator Ani Muscle ◽  
Prolonged Administration ◽  
Potent Effect ◽  
T Testosterone

ABSTRACT Testosterone (T), testosterone-propionate (TP), testosterone-phenyl-propionate (TPP), nandrolone (N) (19-nor-testosterone) and nandrolone-phenyl-propionate (NPP) were compared for their effects on the pseudocholinesterase activities in the liver and serum of castrated male rats. In addition changes in the weight of the seminal vesicle and the levator ani muscle were studied. After daily administration of 1 mg of the hormones for ten days, T and TPP showed a more marked depression of the pseudocholinesterase activity and seminal vesicle than the corresponding nor-derivatives. TP and TPP have approximately similar effects, exceeding those of T. On the levator ani N and NPP were more effective than T and TPP. At identical total doses, administration of all hormones with intervals of more than one day, produced less depression of the pseudocholinesterase activity and less seminal vesicle growth than daily administration. The effects on the levator ani were less influenced by varying intervals. At an interval of four days TPP still had a potent effect on the enzyme activity and the seminal vesicle, whereas T was almost without effect. Prolonged administration showed that the effects on the enzyme activity and the seminal vesicle of N and NPP could not reach the maximum effects of T and TPP respectively.

EFFECTS OF TESTOSTERONE MEDIATED OR MODULATED BY PITUITARY FACTORS

1975 ◽  
Vol 67 (1) ◽  
pp. 71-79 ◽  
Author(s):  
P. DE MOOR ◽  
M. ADAM-HEYLEN ◽  
H. VAN BAELEN ◽  
G. VERHOEVEN
Keyword(s):  
Body Weight ◽  
Testosterone Propionate ◽  
Total Body Weight ◽  
Seminal Vesicles ◽  
Female Rats ◽  
Male Rats ◽  
Intact Male ◽  
Adult Rats ◽  
Organ Weights ◽  
Total Body

SUMMARY Adult rats of both sexes were either gonadectomized or hypophysectomized and gonadectomized. Three to eight weeks later they were treated for 14 consecutive days with oil or with 75 or 200 μg testosterone propionate (TP) per 100 g body weight. The animals were killed and for each sex the gonadectomized animals were compared with the hypophysectomized-gonadectomized animals as far as their NADPH- and NADH-dependent 3α-hydroxysteroid dehydrogenases (3α-HSD) in renal microsomes, transcortin levels in serum and five organ weights relative to total body weight were concerned. For two of the latter, i.e. the relative kidney and prostatic weights, no significant differences were found. Transcortin levels, relative adrenal weights and renal NADPH-dependent 3α-HSD activities were higher in oil-treated gonadectomized animals than in oil-treated hypophysectomized-gonadectomized animals. The opposite was found for the relative weights of uterus and seminal vesicles and renal NADH-dependent 3α-HSD activities. These differences between gonadectomized and hypophysectomized-gonadectomized animals disappeared after TP treatment as far as transcortin levels were concerned but remained for the five other parameters. After gonadectomy sexual differences subsisted for all parameters studied. But whereas intact male rats had higher NADH-dependent 3α-HSD activities than female rats the opposite was found after gonadectomy. After gonadectomy plus hypophysectomy the between sex differences disappeared as far as transcortin levels were concerned but remained in the other parameters studied.

The effect of sex and sex hormones on the infection of rats by Trichinella spiralis

1972 ◽  
Vol 50 (5) ◽  
pp. 597-602 ◽  
Author(s):  
Sarojam K. Mankau ◽  
Raymond Hamilton
Keyword(s):  
Sex Hormones ◽  
Worm Burden ◽  
Testosterone Propionate ◽  
Marked Decrease ◽  
Trichinella Spiralis ◽  
Male Rats ◽  
Normal Male ◽  
Normal Males

Male hooded rats infected with Trichinella spiralis larvae had three times more larvae in the muscles than females. Gonadectomized males injected with stilbestrol had a lower worm burden than normal males. Gonadectomized females injected with testosterone propionate harbored far more worms than normal females. Stilbestrol administered to normal male rats caused a marked decrease in T. spiralis, while testosterone administered to normal females resulted in a significant increase in the number of parasites.

THE EFFECT OF CERTAIN ANDROGENIC STEROIDS ON THE UPTAKE OF RADIOSULPHUR IN A HEALING FRACTURED BONE IN THE RAT

1957 ◽  
Vol 35 (10) ◽  
pp. 771-776 ◽  
Author(s):  
K. Kowalewski ◽  
R. T. Morrison
Keyword(s):  
Normal Control ◽  
Marked Effect ◽  
Testosterone Propionate ◽  
Chondroitin Sulphate ◽  
Significant Rise ◽  
Male Rats ◽  
Testosterone Administration ◽  
Androgenic Steroids ◽  
Intact Rats

The uptake of radiosulphur in the fractured and intact humerus of male rats was determined in normal and in castrated animals and the ratio F/I of radioactivity of fractured (F) to intact (I) bone was calculated. Castration resulted in a significant decrease of F/I ratio, as compared with normal control rats.The effect of testosterone propionate and 17-ethyl-19 nortestosterone (Nilevar) on the S35 uptake in bones was studied. Testosterone administration resulted in a slight increase of the F/I in castrated rats, but had no effect on this ratio in intact animals. Both castrated and intact rats treated with Nilevar exhibited a significant rise of S35 uptake in fractured bones and, consequently, high F/I ratios. It is suggested that Nilevar has a marked effect on the synthesis of chondroitin sulphate in the collagen tissues of healing fractures, as measured by the described S35 uptake technique.

Sign in / Sign up

Export Citation Format

Share Document