scholarly journals Altered sexual differentiation of hepatic uridine diphosphate glucuronyltransferase by neonatal hormone treatment in rats

1979 ◽  
Vol 180 (2) ◽  
pp. 313-318 ◽  
Author(s):  
Coral A. Lamartiniere ◽  
Cindy S. Dieringer ◽  
Etsuko Kita ◽  
George W. Lucier
Keyword(s):  
Enzyme Activity ◽  
Adult Male ◽  
Sexual Differentiation ◽  
Reproductive Tract ◽  
Testosterone Propionate ◽  
Hormone Treatment ◽  
Male Rats ◽  
Ventral Prostate ◽  
Uridine Diphosphate ◽  
Neonatal Administration

The hepatic microsomal enzyme UDP-glucuronyltransferase undergoes a complex developmental pattern in which enzyme activity is first detectable on the 18th day of gestation in rats. Prepubertal activities are similar for males and females. However, postpubertal sexual differentiation of enzyme activity occurs in which male activities are twice those of females. Neonatal administration of testosterone propionate or diethylstilboestrol to intact animals resulted in lowered UDP-glucuronyltransferase activity in liver microsomal fractions of adult male rats, whereas no changes were observed in the adult females and prepubertal male and female animals. Neonatal administration of testosterone propionate and diethylstilboestrol adversely affected male reproductive-tract development as evidenced by decreased weights of testes, seminal vesicles and ventral prostate. Diethylstilboestrol also markedly decreased spermatogenesis. Hypophysectomy of adult male rats resulted in negative modulation of microsomal UDP-glucuronyltransferase and prevented the sexual differentiation of enzyme activity. In contrast hypophysectomy had no effect on female UDP-glucuronyltransferase activity. A pituitary transplant under the kidney capsule was not capable of reversing the enzyme effects of hypophysectomy, therefore suggesting that the male pituitary factor(s) responsible for positive modulation of UDP-glucuronyltransferase might be under hypothalamic control in the form of a releasing factor. Neonatal testosterone propionate and diethylstilboestrol administration apparently interfered with the normal sequence of postpubertal UDP-glucuronyltransferase sexual differentiation.

MODIFICATION OF MALE RAT REPRODUCTIVE TRACT DEVELOPMENT BY A SINGLE INJECTION OF TESTOSTERONE PROPIONATE SHORTLY AFTER BIRTH

1965 ◽  
Vol 50 (2) ◽  
pp. 310-316 ◽  
Author(s):  
H. E. Swanson ◽  
J. J. van der Werff ten Bosch
Keyword(s):  
Reproductive Tract ◽  
Testosterone Propionate ◽  
Pubertal Development ◽  
Testicular Tissue ◽  
Male Rats ◽  
Ventral Prostate ◽  
Male Rat ◽  
Androgenic Activity ◽  
Reduced Rate ◽  
And Control

ABSTRACT Administration of 500 μg testosterone propionate (TP) to male rats within a few days of birth was followed by a reduced rate of growth of the testes and, after initial stimulation, of the seminal vesicles and ventral prostate. While the testes and accessory organs remained permanently smaller in TP-treated rats than in controls, their growth rates showed pubertal accelerations which coincided with those in the controls. On the basis of these criteria, as well as the criterion of spermatogenesis, the time of puberty was not altered by early TP-administration. The weights of the accessory organs considered in relation to the weights of the testes were identical in TP-treated and control rats; testis tubule diameters in TP-treated rats were normal for the age. It is concluded that early TP-administration caused a reduction in the volume of testicular tissue, which involved both tubules and Leydig cells. There were no indications that TP had caused any qualitative changes in the testes: the timing and the pattern of pubertal development (spermatogenesis and androgenic activity) appeared to be normal.

Synergism of testosterone propionate with growth hormone in promoting growth of hypophysectomized rats: effect of sexual differentiation

1990 ◽  
Vol 127 (2) ◽  
pp. 249-256 ◽  
Author(s):  
J. Klindt ◽  
J. J. Ford ◽  
G. J. Macdonald
Keyword(s):  
Sexual Differentiation ◽  
Testosterone Propionate ◽  
Initial Study ◽  
Male Rats ◽  
Growth Enhancement ◽  
Growth Responses ◽  
Liver And Kidney ◽  
Hypophysectomized Rats ◽  
Synergistic Response ◽  
Rates Of Growth

ABSTRACT The effect of testosterone propionate (TP), alone and in combination with porcine GH, on the growth of hypophysectomized rats was investigated. An initial study determined doses of TP and GH which would result in a synergistic response. Hypophysectomized male rats, approximately 40 days of age, received GH at doses of 5, 25 and 62·5 μg/day administered in two injections/day at 08.00 and 16.00 h. At all doses of GH, administration of TP at 100 μg/day significantly enhanced the GH-stimulated rate of growth. This growth enhancement by TP was greatest in combination with GH at 25 μg/day. In a subsequent study, growth responses to 25 μg GH/day and 100 μg TP/day were examined in animals with differing degrees of sexual differentiation. Sex groups were: intact males, males castrated at 11 days of age and females administered 100 μg TP at 3 days of age (masculinized rats), and males castrated at 2 days of age and normal females (non-masculinized rats). In all sex groups, growth of hypophysectomized rats was stimulated by GH. Genetic sex and masculinization did not influence the response to GH. Masculinized hypophysectomized rats exhibited significantly greater rates of growth and final live, empty body, liver and kidney weights than non-masculinized hypophysectomized rats. All sex groups other than normal females responded synergistically to the combination treatment of GH plus TP. Rats that experienced neonatal exposure to testosterone became programmed to respond to testosterone and demonstrated greater rates of growth and body and organ weights when administered the combination of GH plus TP. These data indicate that TP synergizes with GH to promote growth of hypophysectomized rats appropriately programmed to respond. The ability to manifest a synergistic response is a differentiated trait dependent upon exposure to testosterone during the appropriate period of development. The time of differentiation of this ability to respond to testosterone occurs earlier than that for differentiation of body growth. Journal of Endocrinology (1990) 127, 249–256

EFFECTS OF TESTOSTERONE AND NANDROLONE AND SOME OF THEIR ESTERS ON THE PSEUDOCHOLINESTERASE ACTIVITY IN THE LIVER AND SERUM AND ON THE SEMINAL VESICLE AND LEVATOR ANI MUSCLE OF THE RAT

1970 ◽  
Vol 64 (3) ◽  
pp. 531-540 ◽  
Author(s):  
R. S. Leeuwin ◽  
E. Th. Groenewoud
Keyword(s):  
Enzyme Activity ◽  
Seminal Vesicle ◽  
Testosterone Propionate ◽  
Levator Ani ◽  
Daily Administration ◽  
Male Rats ◽  
Levator Ani Muscle ◽  
Prolonged Administration ◽  
Potent Effect ◽  
T Testosterone

ABSTRACT Testosterone (T), testosterone-propionate (TP), testosterone-phenyl-propionate (TPP), nandrolone (N) (19-nor-testosterone) and nandrolone-phenyl-propionate (NPP) were compared for their effects on the pseudocholinesterase activities in the liver and serum of castrated male rats. In addition changes in the weight of the seminal vesicle and the levator ani muscle were studied. After daily administration of 1 mg of the hormones for ten days, T and TPP showed a more marked depression of the pseudocholinesterase activity and seminal vesicle than the corresponding nor-derivatives. TP and TPP have approximately similar effects, exceeding those of T. On the levator ani N and NPP were more effective than T and TPP. At identical total doses, administration of all hormones with intervals of more than one day, produced less depression of the pseudocholinesterase activity and less seminal vesicle growth than daily administration. The effects on the levator ani were less influenced by varying intervals. At an interval of four days TPP still had a potent effect on the enzyme activity and the seminal vesicle, whereas T was almost without effect. Prolonged administration showed that the effects on the enzyme activity and the seminal vesicle of N and NPP could not reach the maximum effects of T and TPP respectively.

EFFECTS OF 17β-HYDROXY-4-ANDROSTEN-19-OL-3-ONE (19-HYDROXYTESTOSTERONE) AND 5α-ANDROSTAN-17β-OL-3-ONE (DIHYDROTESTOSTERONE) ON ASPECTS OF SEXUAL BEHAVIOUR IN CASTRATED MALE RATS

1974 ◽  
Vol 61 (1) ◽  
pp. 105-115 ◽  
Author(s):  
R. F. PARROTT
Keyword(s):  
Adult Male ◽  
Sexual Behaviour ◽  
Testosterone Propionate ◽  
Male Rats ◽  
Organ Weights ◽  
Refractory Periods ◽  
Peripheral Effect ◽  
Rate Of Decline ◽  
The Brain ◽  
Experienced Adult

SUMMARY The ability of 19-hydroxytestosterone propionate (150 μg/day) to maintain sexual behaviour, accessory organ weights and the number of penile spines in experienced adult male rats in the 5 weeks after castration was compared with intact males and castrated animals receiving testosterone propionate (75 μg/day) or oil treatment. In a second experiment a group of male rats receiving dihydrotestosterone propionate (150 μg/day) was also included. 19-Hydroxytestosterone did not maintain ejaculatory performance but animals that ejaculated had refractory periods similar to those in intact and testosterone-treated groups. Dihydrotestosterone, however, slowed the rate of decline of ejaculatory performance but the refractory periods were comparable to those in castrated controls. The former action of dihydrotestosterone was attributed to its stimulatory effect on peripheral structures, especially the penile spines. 19-Hydroxytestosterone was shown to have no peripheral effect at doses up to 1800 μg every other day. The results are discussed in terms of a theory of testosterone action involving aromatization in the brain and 5α-reduction peripherally.

INHIBITORY ACTION OF CORTISONE ON TESTOSTERONE-INDUCED GROWTH OF THE VENTRAL PROSTATE, THE DORSOLATERAL PROSTATE, THE COAGULATING GLANDS AND THE SEMINAL VESICLES IN CASTRATED ADRENALECTOMIZED RATS

1972 ◽  
Vol 69 (2) ◽  
pp. 359-368 ◽  
Author(s):  
Lars-Eric Tisell
Keyword(s):  
Inhibitory Action ◽  
Testosterone Propionate ◽  
Reproductive Organs ◽  
Inhibitory Effect ◽  
Seminal Vesicles ◽  
Male Rats ◽  
Ventral Prostate ◽  
Adrenal Steroids ◽  
Androgenic Effect ◽  
Adrenalectomized Rats

ABSTRACT The weight and histology of the ventral and dorsolateral prostate, the coagulating glands and the seminal vesicles were studied in castrated non-adrenalectomized male rats after sixteen days of daily injections of testosterone propionate and in castrated adrenalectomized rats after daily injections of testosterone propionate alone or in combination with cortisone. Testosterone propionate was given in daily doses of 0.020 mg and cortisone in daily doses of 1 mg, 3 mg or 9 mg. Testosterone alone induced a less pronounced growth of the dorsolateral prostate, the coagulating glands and the seminal vesicles in castrated non-adrenalectomized than in castrated adrenalectomized rats, suggesting an inhibitory effect of adrenal steroids on the action of testosterone. Cortisone which has a weak androgenic effect when given alone, partially counteracted the testosterone induced growth of the accessory reproductive organs in castrated adrenalectomized rats.

scholarly journals The effect of phenobarbital on the submicrosomal distribution of uridine diphosphate glucuronyltransferase from rat liver

1970 ◽  
Vol 117 (2) ◽  
pp. 319-324 ◽  
Author(s):  
G. J. Mulder
Keyword(s):  
Enzyme Activity ◽  
Density Gradient ◽  
Rat Liver ◽  
Microsomal Fraction ◽  
Specific Activity ◽  
Sucrose Density Gradient ◽  
Male Rats ◽  
Uridine Diphosphate ◽  
Triton X

1. The detergent Triton X-100 activates UDP glucuronyltransferase from rat liver in vitro six- to seven-fold with p-nitrophenol as substrate. The enzyme activity when measured in the presence of Triton X-100 is increased significantly by pretreatment of male rats with phenobarbital for 4 days (90mg/kg each day intraperitoneally). If no Triton X-100 is applied in vitro such an increase could not be shown. In all further experiments the enzyme activity was measured after activation by Triton X-100. 2. The Km of the enzyme for the substrate p-nitrophenol does not change on phenobarbital pretreatment. 3. When the microsomal fraction from the liver of untreated rats is subfractionated on a sucrose density gradient, 47% of the enzyme activity is recovered in the rough-surfaced microsomal fraction, which also has a higher specific activity than the smooth-surfaced fraction. 4. Of the increase in activity after the phenobarbital pretreatment 50% occurs in the smooth-surfaced fraction, 19% in the rough-surfaced fraction and 31% in the fraction located between the smooth- and rough-surfaced microsomal fractions on the sucrose density gradient. 5. The latency of the enzyme in vitro, as shown by the effect of the detergent Triton X-100, is discussed in relation to the proposed heterogeneity of UDP glucuronyltransferase.

Effect of cyproterone acetate in comparison to flutamide and megestrol acetate on the ventral prostate, seminal vesicle, and adrenal glands of adult male rats

The Prostate ◽  
1987 ◽  
Vol 11 (4) ◽  
pp. 361-375 ◽  
Author(s):  
M. Fathy El Etreby ◽  
Ursula-F. Habenicht ◽  
Thomas Louton ◽  
Yukishige Nishino ◽  
Helmut G. Schröder
Keyword(s):  
Seminal Vesicle ◽  
Adult Male ◽  
Cyproterone Acetate ◽  
Adrenal Glands ◽  
Megestrol Acetate ◽  
Male Rats ◽  
Ventral Prostate

Inductive influence of testosterone upon central sexual maturation in the rat

Development ◽  
1967 ◽  
Vol 17 (1) ◽  
pp. 171-175
Author(s):  
W. N. Adams Smith ◽  
M. T. Peng
Keyword(s):  
Corpus Luteum ◽  
Sex Hormones ◽  
Adult Male ◽  
Sexual Maturation ◽  
Male Genitalia ◽  
Testosterone Propionate ◽  
Single Injection ◽  
Male Rats ◽  
Corpora Lutea

The influence of the testis and of testosterone upon the development of the male genitalia has been extensively investigated and a number of reviews of this work have been published (Jost, 1960; Burns, 1961). However, Witschi (1957) has stressed the need to distinguish between adult sex hormones, such as testosterone, and the secretions of the immature gonad. The formation of corpora lutea in the ovaries transplanted to adult male rats which had been castrated at birth, and the absence of corpus luteum formation in ovaries transplanted to male hosts bearing transplanted testes in the neck from birth, was reported by Pfeiffer in 1936. Similar observations have been reported by Yazaki (1960) and Harris (1964). A single injection of testosterone propionate has been found to lead to permanent sterility and a loss of corpus luteum formation in the ovaries of mice (Barraclough & Leathern, 1954) and rats (Barraclough, 1961).

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