scholarly journals Sodium-dependent transport of ascorbic acid in U937 cell mitochondria

IUBMB Life ◽  
2013 ◽  
Vol 65 (2) ◽  
pp. 149-153 ◽  
Author(s):  
Catia Azzolini ◽  
Mara Fiorani ◽  
Liana Cerioni ◽  
Andrea Guidarelli ◽  
Orazio Cantoni
Keyword(s):  
Ascorbic Acid ◽  
U937 Cell ◽  
Sodium Dependent ◽  
Dependent Transport

scholarly journals Genetic variation in sodium-dependent ascorbic acid transporters and risk of gastric cancer in Poland

2009 ◽  
Vol 45 (10) ◽  
pp. 1824-1830 ◽  
Author(s):  
Margaret E. Wright ◽  
Gabriella Andreotti ◽  
Jolanta Lissowska ◽  
Meredith Yeager ◽  
Witold Zatonski ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Ascorbic Acid ◽  
Genetic Variation ◽  
Sodium Dependent

scholarly journals Ascorbic Acid Transported by Sodium-Dependent Vitamin C Transporter 2 Stimulates Steroidogenesis in Human Choriocarcinoma Cells

Endocrinology ◽  
2008 ◽  
Vol 149 (1) ◽  
pp. 73-83 ◽  
Author(s):  
Ximei Wu ◽  
Takuma Iguchi ◽  
Norio Itoh ◽  
Kousuke Okamoto ◽  
Tatsuya Takagi ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Sodium Dependent ◽  
Choriocarcinoma Cells ◽  
Human Choriocarcinoma Cells

The sodium-dependent ascorbic acid transporter family SLC23

2013 ◽  
Vol 34 (2-3) ◽  
pp. 436-454 ◽  
Author(s):  
Marc Bürzle ◽  
Yoshiro Suzuki ◽  
Daniel Ackermann ◽  
Hiroki Miyazaki ◽  
Nobuyo Maeda ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Transporter Family ◽  
Sodium Dependent ◽  
Acid Transporter

scholarly journals Perfluoroalkyl Carboxylic Acids Interact with the Human Bile Acid Transporter NTCP

Livers ◽  
2021 ◽  
Vol 1 (4) ◽  
pp. 221-229
Author(s):  
Melissa J. Ruggiero ◽  
Haley Miller ◽  
Jessica Y. Idowu ◽  
Jeremiah D. Zitzow ◽  
Shu-Ching Chang ◽  
...  
Keyword(s):  
Competitive Inhibition ◽  
Enterohepatic Circulation ◽  
Human Bile ◽  
Human Embryonic Kidney Cells ◽  
Elimination Half ◽  
Sodium Dependent ◽  
Km Value ◽  
Bile Acid Transporter ◽  
Dependent Transport ◽  
Chain Lengths

Na+/taurocholate cotransporting polypeptide (NTCP) is important for the enterohepatic circulation of bile acids, which has been suggested to contribute to the long serum elimination half-lives of perfluoroalkyl substances in humans. We demonstrated that some perfluoroalkyl sulfonates are transported by NTCP; however, little was known about carboxylates. The purpose of this study was to determine if perfluoroalkyl carboxylates would interact with NTCP and potentially act as substrates. Sodium-dependent transport of [3H]-taurocholate was measured in human embryonic kidney cells (HEK293) stably expressing NTCP in the absence or presence of perfluoroalkyl carboxylates with varying chain lengths. PFCAs with 8 (PFOA), 9 (PFNA), and 10 (PFDA) carbons were the strongest inhibitors. Inhibition kinetics demonstrated competitive inhibition and indicated that PFNA was the strongest inhibitor followed by PFDA and PFOA. All three compounds are transported by NTCP, and kinetics experiments revealed that PFOA had the highest affinity for NTCP with a Km value of 1.8 ± 0.4 mM. The Km value PFNA was estimated to be 5.3 ± 3.5 mM and the value for PFDA could not be determined due to limited solubility. In conclusion, our results suggest that, in addition to sulfonates, perfluorinated carboxylates are substrates of NTCP and have the potential to interact with NTCP-mediated transport.

scholarly journals Renal and small intestinal sodium-dependent symporters of phosphate and sulphate.

1994 ◽  
Vol 196 (1) ◽  
pp. 167-181
Author(s):  
H Murer ◽  
D Markovich ◽  
J Biber
Keyword(s):  
Apical Membrane ◽  
Transport Systems ◽  
Expression Cloning ◽  
Control Mechanisms ◽  
Intestinal Epithelial ◽  
Physiological Conditions ◽  
Sodium Dependent ◽  
Proximal Tubular ◽  
Small Intestinal ◽  
Dependent Transport

Homeostasis of inorganic phosphate (P(i)) and sulphate (Si) is largely achieved by absorption in the mammalian small intestine and by reabsorption in the proximal tubule of the kidney. Under normal physiological conditions, the kidney appears to play the major role in maintaining the extracellular concentration of these anions. In both epithelia, reabsorption of P(i) and to some extent also of Si underlie a variety of regulatory acute and chronic control mechanisms. Acute regulatory mechanisms are predominantly found in renal P(i) reabsorption, whereas chronic regulation of transepithelial P(i) transport is observed in both tissues. Also, in both epithelia, apically located sodium-dependent transport systems (Na+/P(i) and Na+/Si symport) represent major targets for known regulatory factors. By expression cloning using oocytes of Xenopus laevis, renal and small intestinal Na(+)-dependent phosphate and sulphate transport systems have been identified. Evidence has been obtained that cloned Na+/P(i) and Na+/Si symporters are localized in the apical membrane of proximal tubular or small intestinal epithelial cells respectively. Furthermore, recent results indicate that one of the cloned Na+/P(i) symporters is involved in the physiological and pathophysiological regulation of proximal tubular P(i) reabsorption.

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