scholarly journals In vivo tracking of the tropism of mesenchymal stem cells to malignant gliomas using reporter gene‐based MR imaging

2017 ◽  
Vol 142 (5) ◽  
pp. 1033-1046 ◽  
Author(s):  
Minghui Cao ◽  
Jiaji Mao ◽  
Xiaohui Duan ◽  
Liejing Lu ◽  
Fang Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Mr Imaging ◽  
Reporter Gene ◽  
Malignant Gliomas

scholarly journals In Vivo MR Imaging of Intraarterially Delivered Magnetically Labeled Mesenchymal Stem Cells in a Canine Stroke Model

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e54963 ◽  
Author(s):  
Shan-shan Lu ◽  
Sheng Liu ◽  
Qing-quan Zu ◽  
Xiao-quan Xu ◽  
Jing Yu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Mr Imaging ◽  
Stroke Model

scholarly journals In vivo MR imaging tracking of transplanted mesenchymal stem cells in a rabbit model of acute peripheral nerve traction injury

2010 ◽  
Vol 32 (5) ◽  
pp. 1076-1085 ◽  
Author(s):  
Jun Shen ◽  
Xiao-Hui Duan ◽  
Li-Na Cheng ◽  
Xiao-Mei Zhong ◽  
Ruo-Mi Guo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Mr Imaging ◽  
Peripheral Nerve ◽  
Rabbit Model ◽  
Traction Injury

scholarly journals In vivo MR imaging of magnetically labeled mesenchymal stem cells transplanted into rat liver through hepatic arterial injection

2008 ◽  
Vol 3 (2) ◽  
pp. 72-77 ◽  
Author(s):  
Jinhua Cai ◽  
Xuemei Zhang ◽  
Xin Wang ◽  
Chuan Li ◽  
Guanxin Liu
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Mr Imaging ◽  
Rat Liver

In vivo MR Imaging of Tissue-engineered Human Mesenchymal Stem Cells Transplanted to Mouse: a Preliminary Study

2006 ◽  
Vol 35 (1) ◽  
pp. 101-108 ◽  
Author(s):  
In Kap Ko ◽  
Ho-Taek Song ◽  
Eun-Jin Cho ◽  
Eun Sook Lee ◽  
Yong-Min Huh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Mr Imaging ◽  
Human Mesenchymal Stem Cells ◽  
Preliminary Study

In Vivo MR Imaging of Intravascularly Injected Magnetically Labeled Mesenchymal Stem Cells in Rat Kidney and Liver

Radiology ◽  
2004 ◽  
Vol 233 (3) ◽  
pp. 781-789 ◽  
Author(s):  
Clemens Bos ◽  
Yahsou Delmas ◽  
Alexis Desmoulière ◽  
Anne Solanilla ◽  
Olivier Hauger ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Mr Imaging ◽  
Rat Kidney

scholarly journals In Vivo MR Imaging of Magnetically Labeled Mesenchymal Stem Cells in a Rat Model of Renal Ischemia

2009 ◽  
Vol 10 (3) ◽  
pp. 277 ◽  
Author(s):  
Sung Il Jung ◽  
Seung Hyup Kim ◽  
Hyo-Cheol Kim ◽  
Kyu Ri Son ◽  
Se Young Chung ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Mr Imaging ◽  
Rat Model ◽  
Renal Ischemia

Abstract 17689: Imaging Mitochondrial Function of Mesenchymal Stem Cells Transplanted to the Myocardium

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Federico Franchi ◽  
Karen M Peterson ◽  
Ramasamy Paulmurugan ◽  
Amir Lerman ◽  
Martin G Rodriguez-Porcel
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Mitochondrial Dysfunction ◽  
Reporter Gene ◽  
Mitochondrial Function ◽  
Luciferase Activity ◽  
Firefly Luciferase ◽  
Ischemic Myocardium ◽  
Firefly Luciferase Activity

Introduction: Stem cells have poor survival after transplantation, what can limit the use of these therapies. Mitochondrial dysfunction has been recognized as a limiting event that can lead to cell death. Here, we hypothesized that the mitochondrial function of progenitor cells can be monitored in vivo using reporter gene-based molecular imaging. A better understanding of the biology of transplanted stem cells in the ischemic myocardium can lead to optimization of cell therapies. Methods: Using RT-PCR we identified the antioxidant NAD(P)H:Quinone oxidoreductase (NQO1) enzyme as a marker of mitochondrial dysfunction in mouse Mesenchymal Stem Cells (MSCs) that were treated with a mitochondrial stressor, diethyl maleate (DEM). We then constructed a luciferase reporter gene driven by the NQO1 promoter. After in vitro validation, MSCs were transplanted into a mouse model of ischemia/reperfusion (IR, n=4) and mitochondrial function was monitored by bioluminescence imaging. Results: MSCs treated with DEM had an increase in NQO1 reporter gene signal that inversely correlated with the amount of the mitochondrial by-product adenosine tri-phosphate (ATP, r = -0.9643, p = 0.0028; Fig 1A). Mitochondrial dysfunction was preserved by the use of a mitochondria-targeted antioxidant Mito-Tempo (MT), correcting ATP and NQO1 luciferase signal. In vivo studies showed that MSCs yielded higher Firefly luciferase activity in IR mice compared to sham and this signal was normalized when cells were pre-treated with MT (Fig 1B). Data was confirmed by ex vivo measurement of Firefly luciferase activity, which was 4.9-fold higher in the IR group than in shams, after correction for cell number (Fig 1C). Conclusions: We have developed a novel imaging strategy that allows noninvasive assessment of the mitochondrial status of transplanted stem cells in the ischemic myocardium. Knowing when transplanted cells “suffer’ from mitochondrial dysfunction may allow optimization of these therapies.

Autologous transplantation of mesenchymal stem cells into the portal vein of the miniature pig; a preliminary experiment for NOTES approach

2019 ◽  
Vol 98 (9) ◽  
pp. 350-355
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Therapy ◽  
Portal Vein ◽  
Liver Parenchyma ◽  
Miniature Pig ◽  
Hepatic Diseases ◽  
Study Results

Introduction: There is evidence that mesenchymal stem cells (MSCs) could trans-differentiate into the liver cells in vitro and in vivo and thus may be used as an unfailing source for stem cell therapy of liver disease. Combination of MSCs (with or without their differentiation in vitro) and minimally invasive procedures as laparoscopy or Natural Orifice Transluminal Endoscopic Surgery (NOTES) represents a chance for many patients waiting for liver transplantation in vain. Methods: Over 30 millions of autologous MSCs at passage 3 were transplanted via the portal vein in an eight months old miniature pig. The deposition of transplanted cells in liver parenchyma was evaluated histologically and the trans-differential potential of CM-DiI labeled cells was assessed by expression of pig albumin using immunofluorescence. Results: Three weeks after transplantation we detected the labeled cells (solitary, small clusters) in all 10 samples (2 samples from each lobe) but no diffuse distribution in the samples. The localization of CM-DiI+ cells was predominantly observed around the portal triads. We also detected the localization of albumin signal in CM-DiI labeled cells. Conclusion: The study results showed that the autologous MSCs (without additional hepatic differentiation in vitro) transplantation through the portal vein led to successful infiltration of intact miniature pig liver parenchyma with detectable in vivo trans-differentiation. NOTES as well as other newly developed surgical approaches in combination with cell therapy seem to be very promising for the treatment of hepatic diseases in near future.

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