scholarly journals Cigarette smoke induces promoter methylation of single-stranded DNA-binding protein 2 in human esophageal squamous cell carcinoma

2010 ◽  
Vol 128 (10) ◽  
pp. 2261-2273 ◽  
Author(s):  
Yiping Huang ◽  
Xiaofei Chang ◽  
Juna Lee ◽  
Yong Gu Cho ◽  
Xiaoli Zhong ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Dna Binding ◽  
Esophageal Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cigarette Smoke ◽  
Squamous Cell ◽  
Promoter Methylation ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Single Stranded Dna

scholarly journals A missense mutation in damage-specific DNA binding protein 2 is a genetic risk factor for limbal squamous cell carcinoma in horses

2017 ◽  
Vol 141 (2) ◽  
pp. 342-353 ◽  
Author(s):  
Rebecca R. Bellone ◽  
Jiayin Liu ◽  
Jessica L. Petersen ◽  
Maura Mack ◽  
Moriel Singer-Berk ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Risk Factor ◽  
Dna Binding ◽  
Cell Carcinoma ◽  
Missense Mutation ◽  
Squamous Cell ◽  
Genetic Risk ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Genetic Risk Factor

scholarly journals Knockdown of Ubiquitin-Specific Protease 53 Enhances the Radiosensitivity of Human Cervical Squamous Cell Carcinoma by Regulating DNA Damage-Binding Protein 2

2020 ◽  
Vol 19 ◽  
pp. 153303382092979
Author(s):  
Qifen Zhou ◽  
Xiongbo Yao ◽  
Chunlin Wu ◽  
Shaohua Chen ◽  
Dage Fan
Keyword(s):  
Cell Cycle ◽  
Squamous Cell Carcinoma ◽  
Dna Binding ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Binding Protein ◽  
Cervical Squamous Cell Carcinoma ◽  
Dna Binding Protein ◽  
Specific Protein ◽  
Cycle Arrest

Background: Cervical cancer ranks fourth in incidence and mortality among women. Ubiquitin-specific protein 53 binds to damage-specific DNA binding protein 2 and affects the biological properties of colon cancer. Damage-specific DNA binding protein is involved in nucleotide excision repair, which can repair DNA damage. However, the mechanism by which ubiquitin-specific protein 53 regulates the radiosensitivity of cervical cancer through damage-specific DNA binding protein remains unclear. Methods: Tissue samples from 40 patients with cervical squamous cell carcinoma who received radiotherapy were examined by immunohistochemistry to detect the expression of ubiquitin-specific protein 53, and clinical data were collected for statistical analysis. The cell cycle was detected by flow cytometry in Siha cells transfected with Si-USP53 and exposed to 8 Gy irradiation. Cell viability was determined by the CCK8 method in cells transfected with Si-USP53 and exposed to 0, 2, 4, 6, 8, or 10 Gy. The expression of damage-specific DNA binding protein, cyclin-dependent kinase 1, and cell cycle checkpoint kinase 2 was detected in cells transfected with Si-USP53. Results: The expression of ubiquitin-specific protein 53 in the tissues of patients with cervical squamous cell carcinoma was correlated with the sensitivity to radiotherapy. Knockdown of ubiquitin-specific protein 53 in Siha cells downregulated damage-specific DNA binding protein and caused G2/M cell cycle arrest and decreased the survival rate of cells in response to radiation. Conclusion: Ubiquitin-specific protein 53–induced cell cycle arrest and affected the radiotherapy sensitivity of tumors through damage-specific DNA binding protein.

scholarly journals Analysis of SEPT9 Gene Promoter Methylation Status in Esophageal Squamous Cell Carcinoma

2020 ◽  
Author(s):  
Aida Mirza Aghasi ◽  
Saied Ghorbian
Keyword(s):  
Squamous Cell Carcinoma ◽  
Esophageal Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Promoter Methylation ◽  
Methylation Status ◽  
Gene Promoter ◽  
Sodium Metabisulfite ◽  
Significant Difference ◽  
Cancer Tissues

Introduction: The changes in the level of SEPT9 gene promoter methylation can contribute to the formation of esophageal squamous cell carcinoma. The aim of this study was to evaluate the level of changes in the level of SEPT9 gene promoter methylation in the esophageal squamous cell carcinoma. Methods: In the present case-control study, we collected 75 paraffin blocks of esophageal cancer tissues and 75 paraffin blocks healthy tissues, which were referred to the Noor-E-Nejat and Tabriz International Hospitals during 2013-2017. After DNA extraction and treatment with sodium metabisulfite, the changes of SEPT9 gene promoter methylation assessed using high resolution melting (HRM) technique. The data were analyzed by SPSS 22 and Chi-square test. Results: Our findings did not show a statistically significant difference between the changes of SEPT9 gene promoter methylation in cancer tissues compared to the healthy tissues (P=0.106). Conclusion: This study shows that SEPT9 gene promoter methylation cannot contribute to the esophageal squamous cell carcinoma cancerogenesis.  

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