Alpha fetoprotein is a novel protein-binding partner for caspase-3 and blocks the apoptotic signaling pathway in human hepatoma cells

2009 ◽  
Vol 124 (12) ◽  
pp. 2845-2854 ◽  
Author(s):  
Mengsen Li ◽  
Hui Li ◽  
Chaoying Li ◽  
Sheng Zhou ◽  
Liyuan Guo ◽  
...  
Keyword(s):  
Protein Binding ◽  
Signaling Pathway ◽  
Caspase 3 ◽  
Hepatoma Cells ◽  
Alpha Fetoprotein ◽  
Human Hepatoma ◽  
Human Hepatoma Cells ◽  
Apoptotic Signaling ◽  
Binding Partner ◽  
Novel Protein

The role of alpha-fetoprotein in maintaining the growth of human hepatoma cells

2006 ◽  
Vol 24 (18_suppl) ◽  
pp. 14081-14081
Author(s):  
M. Li ◽  
S. Zhou ◽  
X. Liu ◽  
G. Li
Keyword(s):  
Confocal Microscopy ◽  
Caspase 3 ◽  
Immune Surveillance ◽  
Hepatoma Cells ◽  
Alpha Fetoprotein ◽  
Jurkat Cells ◽  
Human Hepatoma ◽  
Human Hepatoma Cells ◽  
Apoptosis Related Gene

14081 Background: This study was to explored the functional mechanism of alpha-fetoprotein (AFP) in maintaining the proliferation of human hepatoma cells line Bel 7402 and the immunsuppression of lymphocyte Jurkat cells. Methods: Western blot was used to detecting the expression of some apoptosis-related gene, fluorescence labeled AFP and confocal microscopy scanning for receptor binding assay in the membrane in Jurkat cells. Results: It showed that AFP could enhance the expression of survivin and c-ras, but restrain caspase-3 express in Bel 7402 cells by Western blotting analysis. It also showed that AFP could bind to the membrane of Jurkat cells by confocal microscopy scanning, and when treated Jurkat with AFP, it indicated that AFP could repress the expression of survivin and Livin and elevated the activity of caspase-3 in the cells; Co-cultured Bel 7402 cells with Jurkat cells, the expression of tumor necrosis related-apoptosis induced ligand (TRAIL) in Jurkt cells was inhibited, when pretreatment with monoclonal antibody of AFP (Anti-AFP), the restrained effect of TRAIL express and the activity of caspase-3 was elevated in Jurkat cells was removed. It also indicated that Anti-AFP had an ability to block these functions of AFP. Conclusions: AFP has a capability to promote the growth and escape from immune surveillance of human hepatoma cells through enhancing the expression of ras and survivin gene in Bel 7402 cells, suppressing TRAIL, survivin and Livin expressed and upregulated activity of caspase-3 in Jurkat cells. No significant financial relationships to disclose.

The ratio of the caspase 3 cleavage products p20: P17 predicts functional caspase activity in human hepatoma cells following stimulation of death receptors

2000 ◽  
Vol 118 (4) ◽  
pp. A1487
Author(s):  
Stephan F. Schlosser ◽  
Markus Schuler ◽  
Christoph Berg ◽  
Michael Gregor ◽  
Sebastian Wesselborg
Keyword(s):  
Caspase 3 ◽  
Death Receptors ◽  
Hepatoma Cells ◽  
Caspase Activity ◽  
Human Hepatoma ◽  
Human Hepatoma Cells ◽  
Stimulation Of

scholarly journals Sirtuin 3 enhanced drug sensitivity of human hepatoma cells through glutathione S-transferase pi 1/JNK signaling pathway

Oncotarget ◽  
2016 ◽  
Vol 7 (31) ◽  
pp. 50117-50130 ◽  
Author(s):  
Na-Na Tao ◽  
Hong-Zhong Zhou ◽  
Hua Tang ◽  
Xue-Fei Cai ◽  
Wen-Lu Zhang ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Drug Sensitivity ◽  
Hepatoma Cells ◽  
Human Hepatoma ◽  
Glutathione S Transferase ◽  
Sirtuin 3 ◽  
Human Hepatoma Cells ◽  
Jnk Signaling ◽  
Jnk Signaling Pathway

Adenosine-induced caspase-3 activation by tuning Bcl-XL/DIABLO/IAP expression in HuH-7 human hepatoma cells

2010 ◽  
Vol 26 (4) ◽  
pp. 319-330 ◽  
Author(s):  
Dongqin Yang ◽  
Takahiro Yaguchi ◽  
Takashi Nakano ◽  
Tomoyuki Nishizaki
Keyword(s):  
Caspase 3 ◽  
Hepatoma Cells ◽  
Human Hepatoma ◽  
Human Hepatoma Cells
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